Molecular Devices ImageXpress Pico Calibration Kit Manual
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Molecular Devices ImageXpress Pico Calibration Kit Manual

Automated cell imaging system with cellreporterxpress software version 2.5
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ImageXpress® Pico
Automated Cell Imaging System
with CellReporterXpress® Software Version 2.5
Calibration Kit Guide
5063669 E
October 2019

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Summary of Contents for Molecular Devices ImageXpress Pico

  • Page 1 ImageXpress® Pico Automated Cell Imaging System with CellReporterXpress® Software Version 2.5 Calibration Kit Guide 5063669 E October 2019...
  • Page 2 For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.

  • Page 3: Table Of Contents

    Contents Chapter 1: ImageXpress Pico Automated Cell Imaging System Obtaining Support Product Documentation About This Guide Chapter 2: Package Contents Chapter 3: Objective Maintenance Installing an Objective Calibrating an Objective Adjusting an Objective Correction Collar Cleaning an Objective Chapter 4: Filter Cube Maintenance Installing a Filter Cube Calibrating a Filter Cube Appendix A: Optional Parts and Accessories...
  • Page 4 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide 5063669 E...

  • Page 5: Chapter 1: Imagexpress Pico Automated Cell Imaging System

    Chapter 1: ImageXpress Pico Automated Cell Imaging System The Molecular Devices® ImageXpress® Pico Automated Cell Imaging System is an all-in-one platform for automatically acquiring and analyzing images from fluorescently labeled biological samples in plates and slides. It enables you to increase the throughput of your image acquisition and analysis, allowing you to gain insights in minutes.

  • Page 6: Obtaining Support

    Knowledge Base, which contains documentation, technical notes, software upgrades, safety data sheets, and other resources. If you still need assistance after consulting the Knowledge Base, you can submit a request to Molecular Devices Technical Support.

  • Page 7: Product Documentation

    ImageXpress Pico system. It describes how to install and calibrate the objective or filter cube. The information in this guide is subject to change without notice. Molecular Devices recommends that you review the guide on the Knowledge Base for the most up-to-date information.
  • Page 8 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide 5063669 E...

  • Page 9: Chapter 2: Package Contents

    Chapter 2: Package Contents The Calibration Kit includes the following: Item Part Description number 5063669 Calibration Kit Guide 9100-0099 Fluorescent Bead Slide 1-GP-7 Red Plastic Slide 1-GP-8 Green Plastic Slide 1-GP-11 Yellow Plastic Slide For a complete list of the contents of the package, see the enclosed packing list. 5063669 E...
  • Page 10 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide 5063669 E...

  • Page 11: Chapter 3: Objective Maintenance

    The standard objectives in the ImageXpress Pico system are configured and calibrated by Molecular Devices when the instrument is delivered. CAUTION! Replace objectives in their original positions. Tip: Molecular Devices recommends removing and maintaining only one objective at a time. 5063669 E...

  • Page 12: Installing An Objective

    With the instrument power on, do not manually rotate the objective turret. Manually rotating the objective turret can damage the instrument. Molecular Devices precalibrates the objectives to specific slots in the turret. You must install the objectives as follows: Slot...
  • Page 13 Chapter 3: Objective Maintenance Installing an Objective in an Empty Slot You must install objectives in specific slots in the turret. See Installing an Objective on page for details. To install an objective in an empty slot: 1. In the CellReporterXpress software, on the Home page, click Devices.
  • Page 14 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide Replacing an Objective (Different Magnification) You must install objectives in specific slots in the turret. See Installing an Objective on page for details. Because of this, the only reason to replace an objective with another objective of a different magnification is when you are a swapping the 40x objective and the 63x objective in slot 5.
  • Page 15 Chapter 3: Objective Maintenance Replacing an Objective (Same Magnification) You must install objectives in specific slots in the turret. See Installing an Objective on page for details. The typical reason to replace an objective with another objective of the same magnification is when you are replacing a damaged objective.

  • Page 16: Calibrating An Objective

    ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide Calibrating an Objective After you install a new objective from an after-sales purchase, you must calibrate it. A calibration kit, which is included with any after-sales objective purchase, includes the following:...
  • Page 17 Chapter 3: Objective Maintenance 4. Note the status of the objective, which is indicated in the tile. If the tile indicates Not Calibrated, continue to the next step to perform the calibration procedure. If the tile indicates Calibrated, you typically do not need to perform the calibration procedure and you can skip this procedure.

  • Page 18: Adjusting An Objective Correction Collar

    Ensure that all components and access doors are closed before starting the instrument. CAUTION! To prevent skin oils from damaging the optical coatings, Molecular Devices recommends that you wear powder-free disposable gloves when handling objectives and filter cubes.
  • Page 19 Chapter 3: Objective Maintenance Adjusting an Objective Correction Collar for a Plate To adjust an objective correction collar for a slide: 1. In the CellReporterXpress software, on the Home page, click Acquisition. 2. Click Add Protocol. 3. Click New Slide Acquisition. 4.
  • Page 20 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide 10. On the left side of the screen under Steps, click Acquisition Device. 11. On the right side of the screen, click Set Up for Adjustment of Objective Collar. 12. Click OK.
  • Page 21 Chapter 3: Objective Maintenance Adjusting an Objective Correction Collar for a Slide To adjust an objective correction collar for a slide: 1. In the CellReporterXpress software, on the Home page, click Acquisition. 2. Click Add Protocol. 3. Click New Slide Acquisition. 4.
  • Page 22 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide 10. On the left side of the screen under Steps, click Acquisition Device. 11. On the right side of the screen, click Set Up for Adjustment of Objective Collar. 12. Click OK.

  • Page 23: Cleaning An Objective

    Ensure that all components and access doors are closed before starting the instrument. CAUTION! To prevent skin oils from damaging the optical coatings, Molecular Devices recommends that you wear powder-free disposable gloves when handling objectives and filter cubes.
  • Page 24 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide To clean an objective: 1. In the CellReporterXpress software, on the Home page, click Devices. 2. In the Available Acquisition Devices list, select the instrument. 3. Click the Objectives tab. 4. In the tile for the objective you want to clean, click Component Exchange.

  • Page 25: Chapter 4: Filter Cube Maintenance

    Ensure that all components and access doors are closed before starting the instrument. CAUTION! To prevent skin oils from damaging the optical coatings, Molecular Devices recommends that you wear powder-free disposable gloves when handling objectives and filter cubes.
  • Page 26 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide Installing a Filter Cube in an Empty Slot To install a filter cube in an empty slot: 1. In the CellReporterXpress software, on the Home page, click Devices. 2. Click Show Device Options to expand the details for the device where you want to install a filter cube.
  • Page 27 Chapter 4: Filter Cube Maintenance Replacing a Filter Cube (Different Wavelength) To replace a filter cube with a filter cube of a different wavelength: 1. In the CellReporterXpress software, on the Home page, click Devices. 2. Click Show Device Options to expand the details for the device where you want to replace a filter cube.
  • Page 28 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide Replacing a Filter Cube (Same Wavelength) The typical reason to replace a filter cube with another filter cube of the same wavelength is when you are replacing a damaged filter cube.

  • Page 29: Calibrating A Filter Cube

    Chapter 4: Filter Cube Maintenance Calibrating a Filter Cube After you install a new filter cube from an after-sales purchase, you must calibrate it. A calibration kit, which is included with any after-sales filter cube purchase, includes the following: Fluorescent Bead Slide (9100-0099) Red Plastic Slide (1-GP-7) Green Plastic Slide (1-GP-8) Orange Plastic Slide (1-GP-11)
  • Page 30 ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide 4. Note the status of the filter cube, which is indicated in the tile. If the tile indicates Not Calibrated, continue to the next step to perform the calibration procedure. If the tile indicates Calibrated, you typically do not need to perform the calibration procedure and you can skip this procedure.

  • Page 31: Appendix A: Optional Parts And Accessories

    CAUTION! To prevent damaging both the instrument and your sample, do not use any other objectives with the ImageXpress Pico system. Filter Cubes The following filter cubes, which are available from Molecular Devices, are compatible with the ImageXpress Pico system: Filter Part Number...

  • Page 32: Accessories

    ImageXpress Pico Automated Cell Imaging System Calibration Kit Guide Accessories The following accessories, which are available from Molecular Devices, are compatible with the ImageXpress Pico system. Base System Accessories Item Part No. 4-Slide Holder 5068503 Plate Holder 5068504 6-Dish Holder for 35 mm Dishes...
  • Page 33 The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ©2019 Molecular Devices, LLC. Specifications subject to change without notice. Patents: www.moleculardevices.com/patents All rights reserved. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES...

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