Molecular Devices ClonePix 2 Manual
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Molecular Devices ClonePix 2 Manual

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ClonePix
2
ROBOT MANUAL
07MAN1186.B
ECO #: 4100

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Summary of Contents for Molecular Devices ClonePix 2

  • Page 1 ClonePix ROBOT MANUAL 07MAN1186.B ECO #: 4100...

  • Page 2: Table Of Contents

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Contents Table of Figures .................. 4 Introduction to ClonePix 2 ..............5 Technical Specifications ..............6 External Dimensions and Weight ................6 Compressed Air Supply ..................6 Operating Environment ..................6 Electrical Supply ....................7 Electrical Connections ...................
  • Page 3 MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL System Features ....................19 Pre-Power-Up Checklist ..................19 Power-Up Procedures ..................19 Shutdown procedure ................... 20 General Maintenance ................ 21 Regular Maintenance ..................21 General Precautions .................... 22 Cleaning Procedures ................... 22 Removable Parts ................... 22 Cleaning of Instrument Interior ..............

  • Page 4: Table Of Figures

    Table of Figures Figure 1: ClonePix 2 electrical connections ............... 8 Figure 2: ClonePix 2 PC connections ................. 9 Figure 3: ClonePix 2 layout and dimensions ............12 Figure 4: Stacker orientation .................. 13 Figure 5: Wash bottle connections ................14 Figure 6: Front Panel ....................

  • Page 5: Introduction To Clonepix 2

    For detailed information about the software please see the ClonePix 2 Software Application Manual. Note: ClonePix 2 is strictly for research use only and is not intended or recommended for the diagnosis of disease in humans or animals.

  • Page 6: Technical Specifications

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Technical Specifications External Dimensions and Weight Size (excluding ancillary equipment): 1010mm (width) x 900mm (depth) x 1495mm (height) Weight: Robot - 350 kg (maximum) Compressor - 60 kg Compressed Air Supply Minimum Pressure:...

  • Page 7: Electrical Supply

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Electrical Supply European: Voltage: 210 - 250V AC 45-66 Hz single phase Power: Robot - 1250W Connections: IEC Input - Robot Mains / Halogen Heater IEC Output - PC IEC Output - Monitor Fuses: Input - F3 –...

  • Page 8: Electrical Connections

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Electrical Connections Figure 1: ClonePix 2 electrical connections Symbols on Equipment Symbol Meaning Beware moving parts. Refer to Robot Manual for operating instructions. The year of manufacture is given on the serial number label which is located on the right side of the unit.

  • Page 9: Pc Connections

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL PC Connections Mains Input Monitor Camera Ethernet Mouse (USB) Keyboard Printer Figure 2: ClonePix 2 PC connections Molecular Devices > 9 of 39...

  • Page 10: Filter Options

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Filter options Filter set Excitation Emission Cat. No. Suitable for: Wavelength Filter Wavelength Filter (nm) Position (nm) Position X4990 AF430 (Royal Blue) (Cyan) X4991 (standard) CloneDetect FITC* (Blue) (Green) Fluorescein AF488 X4992 (Cyan)

  • Page 11: Robot Enclosure

    ROBOT MANUAL Robot Enclosure ClonePix 2 is designed to provide a controlled environment for sample protection conducive to accurate and reliable mammalian cell picking. Protection within the robot top cover is provided by utilizing a rear mounted HEPA filtered air supply and a front mounted exhaust fan.

  • Page 12: Installation

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Installation Note: Installation is to be undertaken only by Molecular Devices approved personnel. Layout of ClonePix 2 Front view: 1690mm 1010mm Top view: Figure 3: ClonePix 2 layout and dimensions Molecular Devices > 12 of 39...

  • Page 13: Source And Destination Plate Stackers

    ROBOT MANUAL Source and Destination Plate Stackers ClonePix 2 uses a stacker system to hold and process the source and destination plates. The source stacker is located at the rear of the machine and the destination stacker towards the front. The stacker cassettes can accommodate up to 10 microplates. Plates must be loaded into the feed cassette with lids on and well A1 facing towards the imaging station.

  • Page 14: Connecting The Wash Bottles

    Molecular Devices approved engineer on commissioning of ClonePix 2. If it is necessary to disconnect these make sure that they are marked so that the tubes can be re-connected correctly. The outlets on the machine are in the same order as the bottles on the wash station.

  • Page 15: Camera Alignment

    The aim of camera alignment is to achieve pin-to-spot accuracy, relating the image pixel co- ordinates with the robot x, y co-ordinates. Calibration and alignment are performed before ClonePix 2 is dispatched and will be checked by a Molecular Devices approved engineer when the robot is installed.

  • Page 16: Health And Safety

    External Covers Warning – If any of the external covers on ClonePix 2 are removed the power supply is not automatically interrupted. If it is necessary to remove any of the external covers it is essential that the power is switched off first.

  • Page 17: Safety Features

    The door is made from acrylic, and so prevents UV light from passing through during operation. UV Light: ClonePix 2 is fitted with a UV germicidal lamp with timer. This is a 30W linear discharge lamp with a sharply defined output at 253.7nm making the lamp an efficient source of germicidal radiation.

  • Page 18: Noise Levels

    1 meter. Service and Maintenance It is strongly recommend that maintenance be carried out regularly by a Molecular Devices approved service engineer. Maintenance contracts can be obtained from Molecular Devices (New Milton) Ltd. Please see contact details below.

  • Page 19: Initial Operation

    Check that the air supply indicator (on front panel) is on. Once the computer has booted up, click on the ClonePix 2 icon. The first time ClonePix 2 is used, the three axes will sequentially go through their ‘Initialize drives’ routine. This enables the drives to find their respective datum positions.

  • Page 20: Shutdown Procedure

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Shutdown procedure Exit from the ClonePix 2 application by selecting Exit from the File menu on the main setup screen. Close down Windows – Click the Start menu at the bottom of the screen then click Shut Down.

  • Page 21: General Maintenance

    Weekly In any week that ClonePix 2 is not used, it is important to purge the system fluid supply line with sterile water, and run 70% ethanol through the replenishing ethanol wash bath (if fitted). Failure to keep the pumps regularly primed may lead to system malfunction.

  • Page 22: General Precautions

    Cleaning Procedures Removable Parts The design of ClonePix 2 permits all components coming into close proximity with biological material to be removed and cleaned: Picking Head The picking head can be regularly sprayed with 70% ethanol in situ and allowed to dry in the HEPA laminar air flow.
  • Page 23 Leave to air dry. Replace picking head. Important note: Autoclaving the head is not recommended as this can cause deterioration of function. Molecular Devices recommends that each project/group should have at least one picking head. Picking Pins, O-rings and Pin Removal Key...

  • Page 24: Cleaning Of Instrument Interior

    70% Ethanol. Sterilizing Agent (K8080) cannot be used in North America. Abrasive cleaners should not be used, as they will damage the surface of the bed. ClonePix 2 can be left in a laboratory during formaldehyde vapor fumigation at an appropriate concentration.

  • Page 25: Incoming Compressed Air Supply

    The required frequency of sanitization is dependent on usage, but should ideally be conducted weekly (but no more than once a week), and after any period of non-usage. Note: Never clean the ClonePix 2 system fluid lines with bleach. Incoming Compressed Air Supply Compressed air is required for the gentle picking action of the picking pins, and for controlling the movement of the illumination cover.

  • Page 26: Sterility Validation Tests

    Described below are two typical sterility tests conducted on ClonePix 2. 1. Chamber sterility test A ClonePix 2 was prepared using the cleaning procedures described above. A Trypticase Soy Agar* plate (BD Biosciences #221185) was placed open on the robot bed during a prolonged mammalian cell picking run.

  • Page 27: Changing The Picking Head

    Important note: When handling the picking head take care that the pins are not knocked. Damage to pins may produce unreliable results and can cause serious damage to ClonePix 2. If any pins are suspected to have incurred damage please replace them immediately.

  • Page 28: Replacement Parts And Optional Extras

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Replacement Parts and Optional Extras Please refer to the Molecular Devices website for the latest replacement parts and optional extras www.moleculardevices/genetix.com Replacement Parts Code Description X4941 Additional picking head populated with eight X4961 (F1 Pins)

  • Page 29: Reagents And Supplies

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Reagents and Supplies Please refer to the website for the latest reagents and supplies www.moleculardevices/genetix.com CloneDetect Detection Reagents Cat No. Description Pack Size K8295 10000U / 1ml Recombination CloneDetect anti-human IgG detection agent. FITC labeled.

  • Page 30: Clonematrix

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL K8232 CloneDetect anti-mouse detection agent. 649 label. Sterile, azide 10000U / 1ml free. K8233 CloneDetect anti-mouse detection agent. 649 label. Sterile, azide 10000U / 5ml free. In Atomizer applicator K8225 CloneDetect mouse IgG (Fc-Chain) specific. FITC label. Sterile, azide 10000U / 1ml free.

  • Page 31: Petriwell Cell Culture Plates

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL K8775 CloneMedia-PER.C6 (semi-solid media for PER.C6 cells) 1 x 90ml K8650 XP Media (liquid media for cell line expansion of hybridoma/myeloma 500ml cells) K8750 XP Media-CHO (liquid media for cell line expansion of CHO-S cells)

  • Page 32: Other

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL W1557 PetriWell-96 Plate. Black with clear base. EquiGlass. Collagen coated 1/40 W1559 PetriWell-96 Plate. Black with clear base. EquiGlass. Poly-D-Lysine coated 1/40 Other bar-coded plates are available. Please enquire. Other Cat No. Description...

  • Page 33: Troubleshooting

    Manually move the actuator to the middle of the bed Disconnect and reconnect control cable between PC and right side of ClonePix 2 (a small screwdriver may be necessary). Re-boot ClonePix 2 Interior light or UV germicidal lamp will not turn on...

  • Page 34: Glossary Of Terms

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Glossary of Terms BARCODE A unique label for source and destination microplates. Recommended barcoded plate types for colonies suspended in semi-solid medium are PetriWell-1 plates (Catalogue number W1055BC) and PetriWell-6 plates (Catalogue number W1155BC)
  • Page 35 (trans). The Prime Probe is set up by creating a Prime Configuration. PROCESS A standard program for ClonePix 2 to carry out a task such as a series of similar experiments, or a maintenance task. A Process is saved as a .gfp file. PROXIMITY INDICATORS Red lines between colonies on the image that show the nearest neighbour for each colony.
  • Page 36 MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL STERILIZING AGENT Recommended for sterilizing ClonePix 2 and its components and accessories. Catalogue number K8080. (Sterilization Agent (K8080) cannot be used in North America.) TEMPLATE A program from which Processes can be generated. A Template is saved as a .gft file.

  • Page 37: Index

    MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Index Accuracy ..........15 Actuator ..........15, 19 Maintenance ..........18 Air supply ..........19 Maximum ..........6 Airborne noise ......... 18 Mechanical interlock ......... 19 Microplates ..........19 Ball-screw ..........19 Bath ............16 Operating environment .......
  • Page 38 MOLECULAR DEVICES > CLONEPIX 2 ROBOT MANUAL Wash station ........14, 16 Waste bottles .......... 14 Molecular Devices > 38 of 39...

  • Page 39: Contact Details

    Information furnished by Molecular Devices (New Milton) Ltd is believed to be accurate and reliable; however, no responsibility is assumed by Molecular Devices (New Milton) Ltd, for its use; nor for any infringements of patents or other rights of third parties which may result from its use.

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