Molecular Devices ImageXpress Pico Installation Manual page 18

ImageXpress Pico Automated Cell Imaging System Installation Guide
Objective Lenses
The ImageXpress Pico system can be configured with high-quality Leica Fluotar objectives.
Five objectives are currently available.
You can identify the magnification of an objective by the color band:
Objective Magnification
4x
10x
20x
40x
63x
The 40x objective and 63x objective have application-optimized correction collars to
compensate for well bottom thickness or coverslip thickness. The collars have a range of
0 mm to 2 mm correction. Changing this setting adjusts the distances between components
inside the objective barrel. Image quality and resolution are very dependent on properly
setting these collars.
The settings to be used depend on the well bottom thickness of the plate or the coverslip
thickness on the slide on which the specimen is mounted. In general, set the correction collar
for the physical thickness of the plate or slide that you are imaging. The physical thickness can
be determined by the plate specifications from the plate manufacturer.
See Objectives on page 71
18
Color Band
Red
Yellow
Green
Light Blue
Dark Blue
for details.
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