GE ImageQuant LAS 4000 User Manual page 79

Step Operation
Insert the calibration plate appropriate for the light source into the ImageQuant LAS 4000.
Light source
Red Epi light
Green Epi light
Blue Epi light
RGB module
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NIR Epi light
UV Epi light
White Epi light
Close the door of the instrument.
Note:
To perform a Flat Frame calibration, select FlatFrame Calibration in the Option menu.
The FlatFrame Calibration dialog opens.
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Note:
Select the method to calibrate from the drop-down list. Choose which tray positions to calibrate,
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then click Start to begin the calibration.
Once the calibration is complete, click the Close button to close the dialog. The calibration plate
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can now be removed.
ImageQuant LAS 4000 User Manual 28-9607-42 AB
Calibration plate
Cal plate GR (pink)
Cal plate GR (pink)
Cal plate FL (green)
Use the Cal plate that corresponds to the chosen wavelength (red, green or
blue)
Cal plate GR (pink)
Cal plate FL (green)
Cal plate DI (white)
Ensure the the appropriate lights and filter are in place.
Administrator priveleges are required in order to perform a Flat Frame calibration.
4 Operating the ImageQuant LAS 4000
4.16 Creating a new method and performing flat frame calibration
79