Main Specifications; A.4 Main Specifications - GE ImageQuant LAS 4000 User Manual

Classification
Fluorescence dye (Chemi-
fluorescence)
Digitization
1
Attophos cannot be used for detecting nucleic acid on a nylon membrane.
Note:

A.4 Main specifications

CCD
Cooling temperature
Number of gradations
Exposure time
ImageQuant LAS 4000 User Manual 28-9607-42 AB
Reagent name
Alexa 680 Fluorescence:Cy5
Q-dot Fluorescence:DAPI
Dy781 Fluorescence:IR
Alexa 680 Fluorescence:IR
Alexa 700 Fluorescence:IR
1
Attophos Fluorescence:SYBR Green
ECL Plus Fluorescence:SYBR Green
Silver stain Digitization:Trans
CBB stain Digitization:Trans
X-ray film Digitization:Trans
NBT/BCIP Digitization:Epi
With regard to patents owned by third parties related to, among other things, sample
preparation, we recommend that you consult with a lawyer or patent attorney about
obtaining a license from the third parties.
3 200 000 pixels (Fujifilm super CCD)
-25°C
Recorded image: 16 bit
Focusing: 8 bit
1/100 seconds to 2 hours
(Images can be continuously exposed for up to 30 hours using ImageQuant LAS 4000
Control Software.)
A.3 Detection Reagents and corresponding settings
ImageQuant LAS 4000 setting
Method
Red (630nm
Epi)
UV (365nm Epi)
Infrared
(710nm Epi)
Infrared
(710nm Epi)
Infrared
(710nm Epi)
Blue (460nm
Epi)
Blue (460nm
Epi)
White (Trans)
White (Trans)
White (Trans)
White (Epi)
A Appendix
Light Filter
R670
L41
IR785
IR785
IR785
Y515-Di
Y515-Di
Through
Through
Through
Through
Iris
0.85
2.8
0.85
0.85
0.85
0.85
0.85
2.8
2.8
2.8
2.8
127