Exposing Fluorescent Samples (Etbr) - GE ImageQuant LAS 4000 User Manual

4 Operating the ImageQuant LAS 4000
4.5 Exposing chemiluminescent samples
Step Action
Adjust the gradations of the exposed image, then save and print the image.
7
Click the Complete button.
Result: The display returns to the main screen.

4.6 Exposing fluorescent samples (EtBr)

This section describes how to expose ethidium bromide (EtBr) stained samples using the UV
transilluminator (312 nm).
Place a gel sheet, which has been cut out to be slightly larger than the sample, onto the UV trans tray.
Place the sample on the gel sheet. Put the tray into the ImageQuant LAS 4000 and close the door.
Note:
Note:
42
The 605DF40 filter is normally used. If this filter is currently not in the filter turret, it should
be installed. Refer to Section 3.1 Changing or installing a filter, on page
Ensure that the UV transilluminator is installed. For instructions on changing the trans
light source, see Section 3.3.1 Changing the UV transilluminator or white light table, on
page 28.
17.
ImageQuant LAS 4000 User Manual 28-9607-42 AB