GE Amersham Imager 600 Operating Instructions Manual
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GE Amersham Imager 600 Operating Instructions Manual

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Amersham
™
Imager 600
Including:
600, 600 UV, 600 QC, 600 RGB
Operating Instructions
Original instructions

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Summary of Contents for GE Amersham Imager 600

  • Page 1 Amersham ™ Imager 600 Including: 600, 600 UV, 600 QC, 600 RGB Operating Instructions Original instructions...

  • Page 2: Table Of Contents

    Operation ......................Operation flow chart ........................... Turn on the instrument ........................Select tray ..............................Place the tray in the instrument ....................Capture an image ..........................5.5.1 Available methods ......................... 5.5.2 Chemiluminescence capture ..................... Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 3 5.8.6 Summary ............................Turn off the instrument ........................Analysis software ....................Software installation and activation .................... 6.1.1 Amersham Imager 600 Analysis Software installation ..........6.1.2 Amersham Imager 600 Analysis Software registration ..........Software operation ..........................6.2.1 Lane creation ........................... 6.2.2 Background subtraction ......................

  • Page 4: Introduction

    The Operating Instructions provide you with the instructions needed to operate, and maintain Amersham Imager 600 in a safe way. Prerequisites In order to operate Amersham Imager 600 in the intended way, the following prerequisites must be fulfilled: • You have read and understood the safety instructions in this Operating Instruction •...

  • Page 5: Important User Information

    Amersham Imager 600 is a CCD camera system that produces digitial images of samples in gels or membranes in three different modes: chemiluminescence, colorimetric, and fluorescence. Amersham Imager 600 is intended for research use only, and shall not be used in clinical procedures, or for diagnostic purposes. Safety notices This user documentation contains safety notices (WARNING, CAUTION, and NOTICE) concerning the safe use of the product.
  • Page 6 Software items are identified in the text by bold italic text. A colon separates menu levels, thus File:Open refers to the Open command in the File menu. Hardware items are identified in the text by bold text (for example, Power). Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 7: Regulatory Information

    1 Introduction 1.2 Regulatory information Regulatory information This section describes the directives and standards fulfilled by Amersham Imager 600. Manufacturing information The table below summarizes the required manufacturing information. For further infor- mation, see the EU Declaration of Conformity (DoC) document.
  • Page 8 The user is cautioned that any changes or modifications not expressly approved by GE could void the user’s authority to operate the equipment. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.
  • Page 9 • connected to other products recommended or described in the user documentation, • used in the same state as it was delivered from GE, except for alterations described in the user documentation. Environmental Conformity Directive...
  • Page 10 The safety of the LED light sources in this instrument has been assessed by risk analysis in accordance with EN 61010-1. Amersham Imager 600 , depending on configuration, is equipped with some or all light sources in the table below:...

  • Page 11: Safety Instructions

    2 Safety instructions Safety instructions About this chapter This chapter describes safety precautions, safety labels, and emergency procedures for the Amersham Imager 600 instrument, in addition to information on the safe disposal of the instrument. In this chapter Section See page 2.1 Safety precautions...

  • Page 12: Safety Precautions

    Safety precautions Introduction The Amersham Imager 600 instrument is powered by mains voltage, may be equipped with a UV light source, and is used to capture images of samples that may be hazardous. Before installing, operating or maintaining the instrument, you must be aware of the hazards described in the user documentation.
  • Page 13 Do not damage the power supply cord by bending, twisting, heating or allowing them to become pinned under the equipment. Using damaged power cords could result in fire or electric shock. If the power supply cords are damaged, contact your local GE representative for replacements. WARNING Access to power switch and power cord with plug.
  • Page 14 Installation WARNING As the equipment is heavy, contact a GE service person when moving or relocating the main body of the equipment. If the equipment drops, it may be broken, or you may get seriously injured if it drops on your foot.
  • Page 15 2 Safety instructions 2.1 Safety precautions WARNING Use a proper power cord that complies with the local laws and regulations, and is delivered by GE. Do not change power cord to inappropriate one. WARNING In USA and Europe (Belgium, Netherlands, Luxemburg, Germany,...
  • Page 16 In case of thunder, do not touch the power supply plug, as this can result in electric shock. CAUTION Always use appropriate Personal Protective Equipment (PPE) during operation and maintenance of Amersham Imager 600. • Protective eyewear • Protective gloves •...
  • Page 17 Do not disable the interlock of the equipment as UV light can be emitted if the door is opened. NOTICE Both the Amersham Imager 600 Control Software and Amersham Imager 600 Analysis Software do not analyze the image files other than those captured and generated by Amersham Imager 600.
  • Page 18 Do not add and/or remove any files in the folder generated by Amersham Imager 600 with Amersham Imager 600 Control Software and Amersham Imager 600 Analysis Software. Do not modify any folders in which files have been added or removed. This may prevent a correct analysis afterwards.
  • Page 19 Maintenance WARNING Electrical shock hazard. All instrument repairs or modifications should be performed by service personnel authorized by GE. Do not open any covers or replace parts unless specifically stated in the user documentation. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 20 Do not use excessive amounts of liquids for cleaning the Amersham Imager 600 instrument. This may result in instrument malfunction or electric shock. CAUTION Always use appropriate Personal Protective Equipment (PPE) during operation and maintenance of Amersham Imager 600. • Protective eyewear • Protective gloves •...
  • Page 21 2 Safety instructions 2.1 Safety precautions NOTICE When performing maintenance of the equipment, remove the tablet computer. There is risk of injury if it falls on your foot. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 22: Labels

    Labels Introduction A label on the exterior of the Amersham Imager 600 instrument describes the specifica- tions and precautions necessary to safely use the instrument. This section lists and ex- plains the symbols on the label and the additional symbols used on the instrument.
  • Page 23 Occupational Safety and Health Administration (OSHA) has recognized as meeting the legal requirements in USA title 29 of the Code of Federal Regulations (29 CFR) Part 1910.7. Position of label and additional symbols Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 24 For details on symbols see Section 3.4 Instrument hardware description, on page Symbol Description On/Off switch Power switch I: Power ON O: Power OFF DVI-D Connector for DVI-D monitor USB port Ethernet port Caution, hot surface Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 25: Emergency Procedure

    Access to power switch and power cord with plug. Do not block access to the power switch and power cord. The power switch must always be easy to access. The power cord with plug must always be easy to disconnect. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 26: Recycling Information

    Samples are to be disposed of according to local regulations. Disposal, general instructions When taking the Amersham Imager 600 out of service, the different materials must be separated and recycled according to national and local environmental regulations. Recycling of hazardous...

  • Page 27: Declaration Of Hazardous Substances (Dohs)

    EFUP shall be done in accordance with the Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decommissioning. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 28 Indicates that this toxic or hazardous substance contained in at least one of the homoge- neous materials used for this part is above the limit requirement in SJ/T11363-2006. Data listed in the table represents best information available at the time of publication. • Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 29: Instrument Description

    3 Instrument description Instrument description About this chapter This chapter presents an overview of Amersham Imager 600 features and different in- strument configurations. It also contains descriptions of hardware components, software displays, and functions. Note: Due to cosmetic changes to the software, the screen captures in this manual may differ sligtly compared to the acutal software.

  • Page 30: Introduction

    3.1 Introduction Introduction Amersham Imager 600 is an imaging instrument designed to identify and quantify pro- teins or DNA in gels or membranes. A cooled 3.2 megapixel CCD camera is used to capture high resolution digital images of protein and DNA bands in gels and on mem- branes obtained by electrophoresis or western blotting separation methods.

  • Page 31: Feature Overview

    3 Instrument description 3.2 Feature overview Feature overview The following features are offered by Amersham Imager 600: • A highly sensitive CCD camera with 3.2 megapixels • Fast detector cooling capacity • Ready-to-use within 5 minutes after start-up • Tablet computer interface •...

  • Page 32: Illustration Of The Instrument

    3.3 Illustration of the instrument Illustration of the instrument The Amersham Imager 600 instrument is operated from a wireless connected tablet computer. It may also be operated from a wired touch screen or a monitor with mouse. The illustration below displays Amersham Imager 600 setup with tablet computer.

  • Page 33: Instrument Hardware Description

    Instrument hardware description Main components in each configuration Amersham Imager 600 is available in four different configurations. Different parts and functions of Amersham Imager 600 are available depending on configuration. The table below describes the functions and components of the different instrument configurations.
  • Page 34 Run: The LED is lit (blue) when the instrument is operating, or if the Instrument indicator is selected. Error: The LED is lit (red) when the instrument is malfunctioning. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 35 Port for connecting a digital only DVI monitor. USB port Port for additional external devices, for exam- ple a printer. Note: The tablet computer cannot be recharged with the instrument. Ethernet port Connector for a network cable. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 36 For configurations 600, 600 UV, and 600 QC: White light source For configuration 600 RGB: White light plus Blue, Green, and Red light sources Epi light source White light Filter changer Filter changer unit Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 37 ON = 0.5 s / OFF = 1.5 s Mount iPad Air attachment The description below gives the necessary information for the mounting and dismounting of the iPad Air attachment to Amersham Imager 600. Mounting the attachment Note: Place the attachment as illustrated below.
  • Page 38 Push the attachment upward firmly into the guide until you hear a clicking sound. Connect an iPad Air to the attachment. Dismounting the attachment Step Action Push the center of the attachment to release the lock points. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 39 3 Instrument description 3.4 Instrument hardware description Step Action Pull out the attachment downward. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 40: Instrument Software Description

    Instrument software description Introduction The Amersham Imager 600 software is designed to guide you through the workflow of capturing images and analyzing the image data. The software is normally operated on a wireless connected tablet computer placed in the dock on the instrument front. This section describes the main components and functions of the software.

  • Page 41: Description Of The Capture Tab

    Description of the Capture tab Capture view The Capture tab is displayed as the default start screen when Amersham Imager 600 and the tablet computer are turned on and the start up procedure has finished. It allows you to select image capturing method and set details for the exposure. The display changes depending on the choice of method.
  • Page 42 Depending on system configuration you may or may not have access to all methods. The following section describes all available method displays in the Amersham Imager 600 product family. For details on system configurations see Section 3.4 Instrument hardware description,...
  • Page 43 For more information on manual and incremental settings see Set exposure time, on page Start button: use the button to start capturing. High dynamic range checkbox: use the check box for image capture with high dynamic range. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 44 The illustration below shows the specific components of the Capture tab with the Fluo- rescence image capturing method. Depending on selected options, the display changes and unavailable options are hidden (greyed out). For more details of the general components, see Capture view, on page Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 45 • Manual • Advanced • Note: For more information on manual settings see Set exposure time, on page High dynamic range checkbox: Use the check box for image capture with high dynamic range. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 46: Description Of The Library Tab

    Search field: use the field to search for specific files Clear button: use the button to clear the search field Scroll buttons and bar: use the bar or buttons to navigate among files in the displayed list of stored files Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 47 Delete button: use the button to delete the selected (marked) file or files. Rename button: use the button to rename a saved file. Copy/Move button: use the button to copy or move selected files. Open button: use the button to open the selected file. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 48: Description Of The Image View

    Display of the captured image. Note: Incremental and Advanced exposures may result in several images being displayed two at a time as they are exposed. Navigate among the images using forward and backward arrows. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 49 Blue, Green, and Red. Information icon: use to access additional image and exposure information such as image file name and exposure time. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 50 A dialog appears asking to confirm the cancellation. This prevents deleting images by accident. Save button: use the button to save the image. See Section 5.7 Save and manage image files, on page 148 for further details. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 51 Print button: use the button to print the image. Note: The print function requires that the instrument is connected to a USB con- nected printer. For installation of a printer contact a representative from Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 52: Description Of The Analysis Workflow

    Lane profile button: Use the button to display separate graphical lane profiles. Zoom out button: Use the button to zoom out. Zoom in button: Use the button to zoom in. Analyze workflow buttons: Use the buttons to navigate in the analysis workflow. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 53 Open the background subtraction step with the button Background is used to select method for background subtraction. Open the band detection step with the button Band Detection is used to detect the bands in each defined lane. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 54 Open the summary with the button Summary is used to present an overview of the result data from the analysis. Use the Save button to save the analysis data together with the image file. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 55: Description Of Settings Views

    Date & Time button: Use to set date and time on the instrument Focus button: Use to adjust focus Sleep button: Use to set the time when the automatic CCD cooling shall stop after periods of non-use. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 56 Delete button: Use the button to delete one letter at a time. Enter button: Use the button to create a new row. Shift button: Use the button to change to upper case letters keypad. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 57 The time displayed in the Set exposure time window, is by default the exposure time used during the last capture. The time can be set from 0.1 seconds up to 60 minutes. Part Function Cancel button: Use to return to the Capture tab without saving changes. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 58 Use the numeric keys to set the time in the selected field. Arrow buttons: Use the buttons to navigate between minutes, seconds, and 1/10 s in the time units area. Set button: Use the button to save the changes made. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 59: Accessories

    3 Instrument description 3.6 Accessories Accessories Accessories delivered with the instrument The following table shows the accessories of Amersham Imager 600. Depending on configuration the included accessories may vary. Part Description Code no. Relevant for con- figuration: Black tray, marked...
  • Page 60 Amersham Imager 600 Operating Instructions Amersham Imager 600 29-1540-84 All configurations Analysis Software AC power cord (for 19-2448-01 All configurations North America) AC power cord (for Eu- 80-6480-33 All configurations rope) Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 61 AI600 Upgrade 600 to 600 UV 29-0834-22 AI600 Upgrade 600 UV to 600 QC 29-0834-24 600 UV AI600 Upgrade 600 QC to 600 RGB 29-0834-25 600 QC AI600 Upgrade 600 UV to 600 RGB 29-0834-26 600 UV Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 62: Installation

    4 Installation Installation About this chapter This chapter contains information on how to prepare the site for Amersham Imager 600, how to set up network connections, and set system settings. Note: Installation or transport of the Amersham Imager 600 instrument must be per- formed by GE authorized personnel.

  • Page 63: Site Requirements

    4 Do not install the equipment near a window. Avoid direct sunlight. Ensure blinds are attached to nearby windows. 5 Do not place objects near the power outlet to ensure easy access to the power cord for disconnection in case of emergency. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 64 70 dB(A) or lower Fast Lmax ≤60dB(A), and average Leq ≤ 54dB(A) (1 m from the instru- ment) Protection provided by enclosure IP21 Overvoltage category Transient overvoltage category II Rated pollution applied Pollution degree 2 Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 65: Instrument Setup

    Introduction Amersham Imager 600 is initially installed by GE service representatives. Amersham Imager 600 must be secured before long distance transports. Contact GE for instructions before transport or relocation of the instrument. For connecting additional monitors or peripheral equipment contact GE for instructions.

  • Page 66: System Settings

    Capture and Library tabs. For the general components of the Settings view see Section 3.5.5 Description of settings views, on page 55 Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 67: Date And Time

    Change date To change the date setting on the instrument, select the plus or minus buttons next to the Year, Month, and/or Day field, to change the value of year, month or day. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 68 Hour or Minute field, to change the value of hour or minute. Save the changes to date and time Select the Ok button to save the changes to the Date & time view. Select the Cancel button to leave the view without saving changes. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 69: Focus

    Plus and minus buttons: Use the buttons to adjust the brightness value. OK button: Use the button to save the new settings. Cancel button: Use the button to leave the view without saving changes. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 70 Prior to the capture confirm the use of the new focus with Yes. Selecting the No button cancels the adjustments to focus and the normal fixed focus setting (default) is once again in use. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 71: Instrument Indicator

    4.3.3 Instrument indicator 4.3.3 Instrument indicator The Instrument indicator is a useful tool when several Amersham Imager 600 instruments are used in the same location. It identifies which instrument is connected to a certain tablet computer. Select the Instrument indicator button. This activates the Run LED light on the instrument it is connected to.

  • Page 72: Service

    FPGA version The view also displays buttons for software update and export log functions. Update software Follow the instructions below to update the software for Amersham Imager 600. Step Action Connect a USB flash drive containing the update file provided by GE stored at the root level of the USB flash drive.
  • Page 73 To return to the Settings window select the OK button. Confirm the software, Firmware and FPGA version numbers are correctly updated. Export log Follow the instructions below to export a log from Amersham Imager 600. Step Action Select the Export log button.

  • Page 74: Network

    This section contains information on network setup options, virus protection, network settings, and file access. In this section Section See page 4.4.1 Network setup 4.4.2 Network access from instrument 4.4.3 Instrument access from network Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 75: Network Setup

    4.4.1 Network setup 4.4.1 Network setup Virus protection The technical solution for Amersham Imager 600 protects it from virus transfer from in- fected USB flash drives. Each restart of the instrument resets the software to its original installation. Note: If you, for any reason, suspect that the instrument has been infected by a computer virus, restart the instrument as soon as possible.
  • Page 76 Result: The Network view will appear. Select the type of network connection and follow the separate instructions below. Connect to a DHCP network Follow the instructions below, to connect to a DHCP network: Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 77 Result: An automatic connection to the DHCP network is established within a couple of seconds. Tip: Reserve the IP address to Amersham Imager 600 for easier access of its net- work resources. Connect to a Static IP network Follow the instructions below, to connect to a Static IP network:...
  • Page 78 Gateway field and use the keypad to enter the router. • Workgroup field and use the keypad to enter the workgroup. • Select OK to save the changes. Result: The connection to the network will be established within a couple of seconds. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 79: Network Access From Instrument

    4.4.2 Network access from instrument 4.4.2 Network access from instrument To be able to store and access files on a network location, Amersham Imager 600 must have access permission to the specific computer or server where the files are to be stored.
  • Page 80 If password-protected sharing is turned on, you must have a user account and password on your computer for full access to shared items. Password-protected sharing is located in the Control panel under Advanced sharing settings. Pass- word protection is turned on by default. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 81: Instrument Access From Network

    Amersham Imager 600 is equipped with a web tool which allows instrument access from a network. The web tool can be used to copy or delete files on the instrument from a remote location. To be able to access Amersham Imager 600 web tool the following conditions must be fulfilled: •...
  • Page 82 Type the instrument's IP address in the browser address field and select enter. Example: http://192.168.0.25:8080 Result: A page asking for password is displayed. Note: The password can be changed. Press Option in the Login window and follow the instruction in the Options window. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 83 Step Action Type the default password: and select Login. AI600user Result: This opens the Amersham Imager 600 web tool page. Use the search function to limit the list. Search by date: Use the date format YYYY-MM-DD • Example: "2013-11-30" Search by keyword •...

  • Page 84: Operation

    About this chapter This chapter describes how to operate Amersham Imager 600. Prior to operating the Amersham Imager 600 system it is important that you have read and understood the safety information. Make sure to read the relevant safety instructions prior to operation.

  • Page 85: Operation Flow Chart

    The general flow of operation is as follows: Turn on the instrument Select a tray and place sample Place the tray in the instrument Capture the image Save the image file Analyze the image Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 86: Turn On The Instrument

    If relevant, connect a USB flash drive to the instrument for file storage. The USB flash driver is automatically installed when the USB flash drive is connected to Amersham Imager 600. Note: Password protected USB flash drives are not supported. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 87 5 Operation 5.2 Turn on the instrument Step Action Select the icon for Amersham Imager 600 on the desktop screen of the connected tablet computer. Result: 1. A welcome screen appears. 2. The following screen will be displayed. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 88 5 Operation 5.2 Turn on the instrument Step Action Select the Continue button. Result: The following screen will be displayed. Wait for the CCD status to turn green. Result: Image capturing can begin. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 89: Select Tray

    600 QC Gel sheet 600 RGB NOTICE With regards to third party patents related to sample preparation etcetera, we recommend that you consult a lawyer or patent attor- ney on obtaining appropriate licenses. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 90: Place The Tray In The Instrument

    Place the tray in the instrument Place the tray This section describes how to place the sample on the tray and how to place the tray in the correct tray position in Amersham Imager 600. Step Action Select a tray and accessories suitable for the capturing method.
  • Page 91 2 The other side indicates the maximum field of view of 16 x 22 cm for the lower tray position. Use the lower tray position for a larger field of view for one to four samples. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 92 Open the instrument door. Insert the tray in the correct tray position. Note: For Chemiluminescence capturing you may also select the upper tray position (1 in the illustration below). Close the door. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 93: Capture An Image

    Capture an image This section describes how to capture an image with any of the available methods. In this section Section See page 5.5.1 Available methods 5.5.2 Chemiluminescence capture 5.5.3 Colorimetric capture 5.5.4 Fluorescence capture Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 94: Available Methods

    5.5 Capture an image 5.5.1 Available methods 5.5.1 Available methods This section describes how to capture an image. The table below describes the available methods for each Amersham Imager 600 con- figuration: Method Chemiluminescence Chemiluminescence - with colorimetric marker Colorimetric - Epi-illumination...

  • Page 95: Chemiluminescence Capture

    Introduction The chemiluminescence image capture method is available in all configurations of Amersham Imager 600. The instrument captures the light which is emitted during the chemical reaction on the membrane. When a colorimetric marker is present in the sample, an additional color image can be captured using a white light source.
  • Page 96 The software automatically calculates the optimal exposure time for the specified area. To use the full dynamic range of the image file, check High dynamic range. To minimize exposure time, leave High dynamic range unchecked. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 97 In the Capture tab, select Chemiluminescence. Result: The options available for Chemiluminescence capturing are displayed to the right on the screen. Automatic exposure is selected by default and the Exposure options field displays Auto. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 98 Note: Selecting Cancel will cancel the exposure and the Capture view will appear. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 99 5.5.2 Chemiluminescence capture Step Action When the exposure is finished the image view appears displaying the im- age(s). Amersham Imager 600 creates a folder containing the created image files which can be saved. For details see File formats, on page 154.
  • Page 100 To use the full dynamic range of the image file, check High dynamic range. To minimize exposure time, leave High dynamic range unchecked. Select Semi-Auto exposure in the exposure drop down menu. Select Next and wait for the pre-exposure to finish. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 101 Choose (red arrows) to adjust the upper range of the grayscale in the displayed image indicated by the red line in the image histogram. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 102 Result: The selected area is highlighted with a square. Use the right hand side arrow buttons to move the highlighted point or area. Select Start exposure and wait for the exposure to finish. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 103 5.5.2 Chemiluminescence capture Step Action When the exposure is finished the image view appears displaying the im- age(s). Amersham Imager 600 creates a folder containing the created image files which can be saved. For details see File formats, on page 154.
  • Page 104 To change the exposure time, select the exposure time field. Result: The Set exposure time view opens. Enter the new time and select Set to return to the previous view. For detailed instructions Section 3.5.5 Description of settings views, on page Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 105 Select both tabs simultaneously to see a composite color image • of the Sample and Marker images. Select the Save button to save the images. For other options after capturing see Options after capturing, on page 117. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 106 Check the check box Stop when saturated if you wish the exposure to stop when the pixel intensity of the accumulated exposed images reaches satu- ration. Note: The Stop when saturated function overrides the set repetitive exposures. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 107 Auto. This adjustment can be reset with the Reset button. Result: The number of images matching the selected repetitions are displayed as they are exposed. A progress bar indicates the time remaining of the imaging process. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 108 You can navigate among the images by selecting the left or right arrows. Use the intensity tool to check pixel intensity and the contrast tool to adjust contrast and brightness. Amersham Imager 600 creates a folder containing the created image files which can be saved. For details see File formats, on page 154.
  • Page 109 In the Capture tab, select Chemiluminescence. Result: The options available for chemiluminescence capturing are displayed to the right on the screen. If your sample contains a colorimetric marker, then check the Colorimetric marker check box. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 110 The table below shows the available combinations. Colorimetric marker No colorimetric marker Tray position Lower Lower Sensitivity 3 levels 3 levels Image area 1 area 4 areas No. of captures 1 - 12 Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 111 Tray position Sensitivity level Upper position Standard High binning (x4) Lower position Standard High binning (x4) Highest binning (x16) Note: This function increases sensitivity by binning pixels and results in lower image resolution. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 112 Select an area in Image area. This is only available for image capture in the lower tray position with standard sensitivity and without colorimetric maker. Set the number of repetitive exposures in Number of captures by using the plus and minus buttons. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 113 Set the exposure time by pressing value. Result: the Set exposure time view opens Enter the value and select Set to save the value and to return to the previous view. The Reset button resets the value to zero. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 114 5 Operation 5.5 Capture an image 5.5.2 Chemiluminescence capture Step Action Select Start exposure. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 115 The resulting image is displayed in gray scale. was used and one cap- ture was chosen When the exposure is finished the image view appears dis- playing the image. Amersham Imager 600 creates a folder for the image files. For details see File formats, on page 154.
  • Page 116 Use the intensity tool to check pixel intensity and the contrast tool to adjust contrast and brightness. Amersham Imager 600 creates a folder for the image files. For details see File formats, on page 154.
  • Page 117 To analyze the image, select Analyze. Section 5.8 Analyze the image, on page 160 for further instructions. • To print the image, select Print. • To discard the image without saving select Done and confirm the following dialog. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 118: Colorimetric Capture

    Both options use white light imaging sources. For Epi-illumination the light source is lo- cated at the top of the Amersham Imager 600 interior. For Trans-illumination the sample is illuminated from the light source at the bottom of the instrument interior. When using Trans-illumination, the intensity data is converted to optical density (OD values), which provides a more quantitative result compared to Epi-illumination.
  • Page 119 Follow the instructions in the table to perform colorimetric capture. Step Action In the Capture tab, select Colorimetric. Result: The options available for colorimetric capturing are displayed to the right on the screen. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 120 For Trans-illumination place a diffuser board at the bottom of the instrument with the matte side up, before capturing. Select Start. Result: A progress bar appears on the screen indicating the time remaining of the imaging process. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 121 5.5.3 Colorimetric capture Step Action When the exposure is finished the image view appears displaying the im- ages. Amersham Imager 600 creates a folder containing the created image files which can be saved. For details see File formats, on page 154.

  • Page 122: Fluorescence Capture

    Fluorescence capturing is performed using a light source and emission filter. Note: Fluorescence capture is only available with Lower tray position. The methods available in Amersham Imager 600 configurations are described in the following table: 600 UV 600 QC...
  • Page 123 Semi-automatic exposure is not available for multichannel fluorescence capture. The intensity of the im- Select Manual exposure and set the exposure time man- age is inadequate after ually. automatic exposure or a known exposure time is required Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 124 To use the full dynamic range of the image file, check High dynamic range. To minimize exposure time, leave High dynamic range unchecked. Note: This function is only effective with the Auto and Semi-Auto exposure settings. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 125 If more than one detection channel was selected, the Exposure time button displays Set. If only one detection channel was selected, the button displays the latest set exposure time. Result: This opens the Set exposure time view. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 126 Result: This will close the view. For details see, Set exposure time, on page Select Start. A progress bar appears on the screen indicating the time re- maining of the imaging process and the selected exposure option. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 127 Action When the exposure is finished the image view displays the images. Amersham Imager 600 creates a folder containing image files which can be saved. The display and number of images created depends on the number of detection channels selected in step 2.
  • Page 128 Result: The options available for fluorescence capturing are displayed to the right on the screen. Check the Epi-RGB radio button, and then check the relevant check box of Blue, Green, or Red. Check the Colorimetric marker check box. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 129 Proceed to the final step of this instruction. If Semi-Auto exposure was chosen, select Next and wait for the pre-exposure to finish. Result: A pre-exposed image appears. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 130 (red arrows) to adjust the upper range of the grayscale in the displayed image indicated by the red line in the image histogram. Note: The colorimetric marker is not displayed in the preview. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 131 Select Start exposure and wait for the exposure to finish. When the exposure is finished the image view appears displaying the images. Amersham Imager 600 creates a folder containing the created image files which can be saved. For details see File formats, on page 154.
  • Page 132 Advanced exposure mode is available with only one channel of Epi RGB or a custom channel under Fluorescence RGB capture. If the sample contains a colorimetric marker, then check the Colorimetric marker box. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 133 The table below shows the available combinations. Colorimetric marker No Colorimetric marker Tray position Lower Lower Sensitivity 3 levels 3 levels Image area 1 area 4 areas Noumber of captures 1 - 12 Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 134 This function increases sensitivity by binning pixels and results in lower image resolution. Select an area in Image area. This is only available for image capture in the lower tray position with standard sensitivity and without colorimetric maker. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 135 Result: this opens the Set waiting time view. Enter the value and select Set to save the value and to return to the previous view. The Reset button resets the value to zero. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 136 Set the exposure time by pressing value. Result: the Set exposure time view opens Enter the value and select Set to save the value and to return to the previous view. The Reset button resets the value to zero. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 137 5 Operation 5.5 Capture an image 5.5.4 Fluorescence capture Step Action Select Start exposure. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 138 When the exposure is finished the image view appears dis- playing the image. Amersham Imager 600 creates a folder for the image files. For details see File formats, on page 154.
  • Page 139 Navigate among the images by selecting the left or right arrows and check pixel intensity with the in- tensity tool. Amersham Imager 600 creates a folder for the image files. For details see File formats, on page 154.
  • Page 140 Fluorescence Custom The Custom option is designed to allow for various customized combinations of wave- lengths of the illuminating light and filters. Please contact your GE representative for more details as the customized methods require service. Fluorescence Trans-UV Capturing with the Fluorescence image capturing method option Trans-UV is performed using the UV Trans Tray (transparent tray) and a gel sheet.
  • Page 141 Result: A progress bar appears on the screen indicating the time remaining of the imaging process and the exposure time. Proceed to the final step of this instruction. If Semi-Auto exposure was selected, select Next. Result: A low resolution preview appears. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 142 Choose (red arrows) to adjust the upper range of the grayscale in the displayed image indicated by the red line in the image histogram. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 143 Imager 600 creates a folder containing the image files which can be saved. For details see File formats, on page 154. Select the Save button to save the images. For other options after capturing see Options after capturing, on page 117. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 144: View The Image

    A non displayed channel is indicated with a black text and gray frame. Image tools menu: Use the buttons to choose tools to modify the image display and to examine the image. The selected image tool options. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 145 If a different tool is selected when the image is zoomed in, the selected zoom will be kept until saved. Use the Reset button to return to the original zoom view. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 146 Use the blue and red arrows to modify the contrast. Note: For samples with colorimetric marker, use the contrast tool for setting the contrast of the chemiluminescent sample. The selected contrast is saved with the combined color im- age. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 147 Image files may be printed directly from the result image screen. The printing option requires a printer to be connected to the instrument. Print using the Print button. Contact your GE representative for further details on printer installations. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 148: Save And Manage Image Files

    After capturing the image(s) can be saved. You can also copy, move, rename, delete, search, sort, and open files. If the Amersham Imager 600 instrument is connected to a network, you can access and manage files stored on the network location.

  • Page 149: Save Locations

    On the instrument Note: Amersham Imager 600 is equipped with a flash drive that allows for storage of a finite number of images on the instrument. It is necessary to regularly re- move images from the instrument to ensure available storage space. If the instrument flash drive runs full, the oldest images will automatically be removed when a new image is captured.
  • Page 150 Use this location button. Ensure that the path to this location appears in the Location field. Select the OK button to save the added location. The Cancel button will leave the view without saving changes. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 151 The network settings must be properly configured before a network folder can be added as a location for saving files. For information on how to configure a network, see Section 4.4 Network, on page To save the changes, select the OK button. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 152: Save Images

    Result: The Save view opens. The automatically generated image name(s) are displayed in the lower part of the view. Note: If an edited (split) image is saved then the screen will display the edited (split) suffix. Example: Note: Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 153 To optimize the instrument's life time and to minimize unnecessary power consumption, shut down the instrument when image captures and analyses have been completed for the day. If the Amersham Imager 600 instrument is used around the clock, make sure to restart the system at least once a day to remove temporary files that may take up unnecessary memory space.
  • Page 154 Marker image in col- • .jpg or, and with set contrast, for presentation purposes Note: The .jpg image is included in the folder but it is not displayed on the screen. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 155 Marker image in color, and • .jpg with set contrast, for presentation purpos- Note: The .jpg image is included in the folder but it is not displayed on the screen. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 156 Blue + Green + Red: A composite • .jpg image of fluorescence data, in color, and with set contrast Note: The composite image is displayed if two or three tabs are selected simultaneously. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 157: File Manage Options

    The Select all and Deselect all buttons are used to select or deselect all of the files in the library. Delete files Follow the instruction to delete files from the device. Step Action Select the file(s) to delete. Select the Delete button. Result: The following window will appear: Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 158 Select the Copy button, to copy the file(s) to the desired location. Select the Move button, to move the file(s) to the desired location. Note: When a file is copied, the file will remain in the original location, thus be stored in two places. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 159 If desired, use the Image tools to adjust the view of the image. For detailed information on the Image tools, see Image tools, on page 145. When finished, select the Done button to go back to the Library tab. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 160: Analyze The Image

    This section describes how to analyze the image using the analysis tool. The analysis workflow in Amersham Imager 600 allows you to perform a step-by-step analysis of the image data and to save the analysis results. The workflow is performed in five steps and the final result is presented in a summary.
  • Page 161 5 Operation 5.8 Analyze the image Part Function Illustration The current step in the analysis workflow (highlighted). Back button: Use the button to close the analysis workflow. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 162 Options and steps associated to the current step in the analysis. Help icon: Use to reveal additional information on the selected step in the analysis. Next button: Use to open the next step in the analysis workflow. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 163: Lane Creation

    Select the minus and plus buttons adjacent to Number of lanes to increase or decrease the number of grid lanes to correspond with the number of lanes in the image. Select a grid corner circle. Result: The selected corner circle is highlighted. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 164 When you are done select the Next button or select the Background button in the workflow. The Back button will bring you back to the captured image. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 165: Background Subtraction

    Rubber band Use this option to subtract the background below a base- line between the lowest points on the lane profile, as though a rubber band was stretched under the profile. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 166 If an image of the sample is displayed, select the lane profile button. Result: A screen will appear displaying the lane profile graphically, with the current lane number displayed in the drop down list and the lane profile button in blue. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 167 Rolling ball, and then select the Radius plus or minus buttons to set the • radius for the virtual rolling ball eraser to set the new baseline of the graph. Tip: The smaller the radius the more background will be removed. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 168 To view the result in any lane, select the lane number to view in the drop down menu. When you are done select the Next button or select the Bands button in the workflow. The Previous button will bring you back to the previous step. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 169: Band Detection

    If too many bands are detected, then use the Sensitivity minus button to decrease the sensitivity. If too few bands are detected, then use the Sensitivity plus button to increase the sensitivity. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 170 Repeat actions in step 3 for other detection channels, if relevant. When you are done detecting bands press the Next button or select the MW button in the workflow. The Previous button will bring you back to the previ- ous step. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 171: Molecular Weight Assignment

    Add a missing marker by selecting Add new... button. See instructions • below. Edit a marker by selecting the Edit button. See instruction below. • Deselect any missing bands in the sample marker lane by unchecking rele- vant check boxes. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 172 Result: The value will appear in the bottom of the list to the left on the screen. Select the Save button to save the added values and to return to the MW Calibration view. Select Cancel to leave without saving changes. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 173 Select the Save button to save the edited values and to return to the MW Calibration view. Select Cancel to leave without saving changes. Delete will delete the entire marker group. Note: To delete a value, select the X next to the value. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 174: Normalization

    Control bands can only be selected in single channel view. When you are done select the Next button or select the Summary button in the workflow. The Previous button will bring you back to the previous step. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 175: Summary

    Lane% - the relative band volume within a lane (in percentage) Normalized value - the band volume relative to the control band volume Mw - the calculated Mw Rf - the relative mobility of the band Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 176 In addition to the image file(s), analyzed data is saved with a CSV file and the summary view is saved as a pdf file. Section 5.7 Save and manage image files, on page 148 for details. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 177: Turn Off The Instrument

    If the Amersham Imager 600 instrument is used around the clock, make sure to restart the system at least once a day to remove temporary files that may take up unnecessary memory space.

  • Page 178: Analysis Software

    Analysis software About this chapter This chapter describes the installation and the operation of Amersham Imager 600 Analysis Software, which is available as an independent software package. Amersham Imager 600 Analysis Software can be installed on computers using Windows 7 or Win- dows 8.1 operating system.

  • Page 179: Software Installation And Activation

    1 Install the Amersham Imager 600 Analysis Software. 2 A registration number is presented by the Amersham Imager 600 Analysis Software. 3 Go the main instrument to create a license key with the Amersham Imager 600 Control Software. 4 Activate the Amersham Imager 600 Analysis Software by registering with the license key.

  • Page 180: Amersham Imager 600 Analysis Software Installation

    6.1.1 Amersham Imager 600 Analysis Software installation Step Action Insert the installation DVD In the Installer, locate and double-click the file, Amersham Imager 600 Analysis Software.msi Result: Amersham Imager 600 Analysis Software InstallShieldWizard dialog box appears. Click Next. Read the license agreement. If the license agreement is not acceptable, please contact your GE representative.
  • Page 181 6 Analysis software 6.1 Software installation and activation 6.1.1 Amersham Imager 600 Analysis Software installation Step Action Define the software destination folder in the Destination Folder dialog as described below. If the default folder C:\Program Files (x86)\ is acceptable, click Next.
  • Page 182 6 Analysis software 6.1 Software installation and activation 6.1.1 Amersham Imager 600 Analysis Software installation Step Action Click Finish in InstallShield Wizard. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 183: Amersham Imager 600 Analysis Software Registration

    6.1 Software installation and activation 6.1.2 Amersham Imager 600 Analysis Software registration 6.1.2 Amersham Imager 600 Analysis Software registration Follow the instructions below to register the Amersham Imager 600 Analysis Software. Step Action After the installation, the software starts automatically and shows the Reg- istration dialogue box.
  • Page 184 6.1 Software installation and activation 6.1.2 Amersham Imager 600 Analysis Software registration Step Action Select to open the Setting Screen on the Amersham Imager 600 Control Software. Select Analysis software license Result: Analysis software license appears. Enter the Serial number and the Registration number.
  • Page 185 6 Analysis software 6.1 Software installation and activation 6.1.2 Amersham Imager 600 Analysis Software registration Step Action Press Register. Press Yes when confirmation of the registration appears. Result: The 10-digit License key number is generated. Make a note of the 10-digit License key number.
  • Page 186 6 Analysis software 6.1 Software installation and activation 6.1.2 Amersham Imager 600 Analysis Software registration Step Action Enter the Serial number and the License key to the registration screen. Press Register. The registration of the analysis software is now complete.

  • Page 187: Software Operation

    Amersham Imager 600 Analysis Software. The analysis software runs independently on a Windows PC. The workflow in the Amersham Imager 600 Analysis Software allows you to perform a step-by-step analysis of the image data and to save the results in the same way as with the Image analysis function in the Amersham Imager 600 Control Software.
  • Page 188 6 Analysis software 6.2 Software operation Part Function The current step in the analysis workflow is highlighted in blue Use the Close button to close the analysis workflow Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 189 Part Function Image tools button: Use to access the image tools which modify display settings and to view image details. Display Zoom Contrast Intensity For more information see Image tools, on page 145. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 190 Options and steps associated with the current step in the analysis. Help icon: Use to show additional information about the selected step in the analysis. Next button: Use to proceed to the next step in the analysis workflow. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 191: Lane Creation

    Select the minus and plus buttons adjacent to Number of lanes to increase or decrease the number of grid lanes to correspond with the number of lanes in the image. Select a grid corner circle. Result: The selected corner circle is highlighted. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 192 If the value is 100% then the grid contains only the lanes. Using a lesser per- centage creates an empty space between lanes. When you are done select the Next button or select the Background button in the workflow. The Previous button returns you to the captured image. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 193: Background Subtraction

    Use this option when no background should be subtracted from the image. Rubber Use this option to subtract the background below a baseline between the band lowest points on the lane profile, as though a rubber band was stretched under the profile. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 194 Rolling Use this option to subtract the background below the graph as if erasing it ball using the radius of a ball. A small radius will subtract more background than a large. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 195 To confirm that each background subtraction options works, check Show background check box This function shows the background level calculated by each option. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 196 If Rolling ball is selected, use the Radius plus or minus buttons to set the radius of the virtual rolling ball eraser. Tip: The smaller the radius the more background will be removed. Result: The lane profile view is displayed with the background subtracted. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 197 To view the result in any lane, select the lane number in the drop down menu. When ready, press Next or select the Bands in the workflow. Pressing Previ- ous takes you back one step. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 198: Band Detection

    If too many bands are detected, then use the Sensitivity minus button to decrease the sensitivity. If too few bands are detected, then use the Sensitivity plus button to increase the sensitivity. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 199 Repeat actions in step 3 for other detection channels, if relevant. When you are done detecting bands press the Next button or select the MW button in the workflow. The Previous button will bring you back to the previ- ous step. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 200: Molecular Weight Assignment

    Add a missing marker by selecting Add new... button. See instructions • below. Edit a marker by selecting the Edit button. See the instructions below. • Deselect any missing bands in the sample marker lane by unchecking rele- vant check boxes. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 201 Result: The value will appear in the bottom of the list to the left on the screen. Select the Save button to save the added values and to return to the MW Calibration view. Select Cancel to leave without saving changes. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 202 Select the Save button to save the edited values and to return to the MW Calibration view. Select Cancel to leave without saving changes. Delete will delete the entire marker group. Note: To delete a value, select the X next to the value. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 203: Normalization

    Control bands can only be selected in single channel view. When you are done select the Next button or select the Summary button in the workflow. The Previous button will bring you back to the previous step. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 204: Summary

    Lane% - the relative band volume within a lane (in percentage) Normalized value - the band volume relative to the control band volume Mw - the calculated Mw Rf - the relative mobility of the band Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 205 To overwrite the analysis of the file opened, press Save. Result: The image file is updated with the analysis information. To create a new file set, press Save as. Result: The Save image as view appears. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 206 Result: In addition to the image file(s), analyzed data is saved with a CSV file and the summary view is saved as a pdf file. Section 5.7 Save and manage image files, on page 148 for details. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 207: Maintenance

    7 Maintenance Maintenance About this chapter To ensure optimal performance, Amersham Imager 600 and accessories should be maintained regularly. This chapter describes the maintenance that should be performed on a regular basis. For all safety cautions and notices relevant to maintenance please see...

  • Page 208: Instrument

    7.1 Instrument Instrument Use a soft dry cloth to clean the exterior of Amersham Imager 600. For marks that are hard to remove, wipe with a soft cloth slightly dampened with water and a neutral de- tergent, or 70% ethanol, then wipe with a new clean, dry cloth.

  • Page 209: Accessories

    7 Maintenance 7.2 Accessories Accessories Introduction Amersham Imager 600 accessories must be cleaned after use. This section describes recommended cleaning agents and how to clean the accessories. Recommended cleaning agents NOTICE Cleaning agents should be for biochemical use to avoid that residues fluoresce when images are captured.
  • Page 210 Cleaning gel sheets After use, wash the gel sheet with mild detergent, rinse with water then dry well. Note: A gel sheet can be reused about 20 times. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 211: Regular Inspections

    7 Maintenance 7.3 Regular inspections Regular inspections Regularly inspect Amersham Imager 600 to ensure the following: • The power supply plug is firmly secured in the power outlet. • The power cord and supply plug do not become overheated. •...

  • Page 212: Troubleshooting

    Power switch on the right hand side, and turn it back on again after about ten seconds. 3 If the error persists refer to this troubleshooting guide for possible solutions. 4 If the error cannot be resolved with this troubleshooting guide, contact your GE representative. Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 213: General Problems

    GE representative The LED blinks If... Then... The ERROR LED blinks a hardware error If an error code is displayed, make a note occurred of it and contact your GE representative. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 214 The instrument does not appear to re- The instrument is not connected to the spond to software control tablet computer Use the flash indicator tool to identify which instrument is connected to a cer- tain tablet computer Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 215: Problems With Image Quality

    Adjust the focus temporarily. See Section 4.3.2 Focus, on ly adjusted page If the problem persists contact your GE representative. The tray, white insert or Clean the tray, white insert or the window covering the window covering the light source.
  • Page 216 Unevenness appears on the image If... Then... The LED is deteriorated Turn off the instrument and contact your GE representa- from age tive A lamp in the UV transil- Turn off the instrument and contact your GE representa- luminator is broken...

  • Page 217: Problems With The Software

    The buttons do not appear on an external monitor If... Then... The monitor resolution is too low Use monitors with SVGA resolution or higher Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 218 No or few button presses are registered. Use gentle and distinct touches on the tablet computer to control buttons in the software. A light and short tap is best registered by the software. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 219 8 Troubleshooting 8.3 Problems with the software If... Then... The Amersham Imager 600 icon has been Connect to the same network as accidently removed from the tablet Amersham Imager 600. Open the Safari™ computer. web browser and type in the IP address of the instrument in the address field.

  • Page 220: Specifications

    9 Specifications Specifications About this chapter This chapter contains the technical specifications for the Amersham Imager 600 system. Imaging specifications Parameter Data Image sensor Peltier cooled, 3.2 megapixel CCD Image sensor temperature -25°C Cooling down time <5 minutes Lens F0.85/43 mm...
  • Page 221 The following table describes the environmental requirements for Amersham Imager 600. CAUTION Do not use the instrument in a room with a temperature above +28°C. Higher temperatures does not allow the CCD to cool down properly (to -25°C). Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 222 When the above conditions cannot be satisfied, take appropriate actions. Transportation/storage Temperature: -25°C to 70°C conditions Humidity: 5% to 95% RH (no dew condensation) Note: When the above conditions cannot be satisfied, take appropriate actions. Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 223 70 dB(A) or lower Fast Lmax ≤60dB(A), and average Leq ≤ 54dB(A) (1 m from the instru- ment) Protection provided by enclosure IP21 Overvoltage category Transient overvoltage category II Rated pollution applied Pollution degree 2 Amersham Imager 600 Operating Instructions 29-0645-17 AD...

  • Page 224: Index

    Mainenance precautions, 19 Operation precautions, 16 Personal protection, 13 Keypad, 56 Safety precautionsGeneral precautions General precautions, 12 Labels, 22 Sample and tray combina- LED Indicators, 37 tions, 89 Save location, 149 Save the image, 148 Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 225 User information, important, 5 File formats, 154 Keypad, 56 Specifications, 220 Virus protection, 75 Start screen, 41 Web tool, 81 Tool Windows 7, 79 Web, 81 Tools Contrast tool, 146 Zoom tool, 145 Amersham Imager 600 Operating Instructions 29-0645-17 AD...
  • Page 226 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare repre- sentative for the most current information.

This manual is also suitable for:

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