GE AxiChrom 50 Operating Instructions Manual
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GE AxiChrom 50 Operating Instructions Manual

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GE Healthcare
Life Sciences
AxiChrom™ 50, 70 and 100
columns
Operating Instructions
Original instructions

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Summary of Contents for GE AxiChrom 50

  • Page 1 GE Healthcare Life Sciences AxiChrom™ 50, 70 and 100 columns Operating Instructions Original instructions...

  • Page 3: Table Of Contents

    Preparing the slurry, priming and packing the column using ÄKTAavant ....Manual packing of the column ...................... Performance evaluation of the column ..................Unpacking a packed column ......................Cleaning-in-place (CIP) ........................Sanitization .............................. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 4 Parts list and diagrams AxiChrom 50 column ..........Parts list and diagrams AxiChrom 70 column ..........Parts lists and diagrams AxiChrom 100 column ..........Tubing kits and connections ................Index ........................AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 5: Introduction

    The 70 and 100 columns are mounted on a pivot stand, which allows easy handling and unpacking. AxiChrom 50, 70 and 100 come in two column tube lengths to allow bed heights between 10-30 cm and 30-50 cm. The columns are validatable and use materials classified according to USP Class VI and FDA CFR 177.

  • Page 6: Important User Information

    Always keep the Operating Instructions at hand when using AxiChrom 50, 70 and 100 columns. Do not operate AxiChrom 50, 70 and 100 columns in any other way than described in the user documentation. If you do, you may be exposed to hazards that can lead to personal injury, and you may cause damage to the equipment.
  • Page 7 1 Introduction 1.3 Important user information Intended use AxiChrom 50, 70 and 100 are designed for chromatographic separations in process de- velopment, scale-up trials, and small scale production. The columns shall be used in accordance with the safety instructions and technical specifications given in this Operating Instructions so as to not cause injury or to not damage the column or other equipment.

  • Page 8: Regulatory Information

    File:Open refers to the Open command in the File menu. Hardware items are identified in the text by bold text (e.g., Power switch). Regulatory information This section describes the directives and standards that are fulfilled by AxiChrom 50, 70 and 100. Manufacturing information...
  • Page 9 CE-marked products recommended or described in the user documentation, and • used in the same state as it was delivered from GE Healthcare, except for alterations described in the user documentation. The Declaration of conformity is valid only for systems that are marked with the CE-marking.

  • Page 10: Safety Instructions

    This Operating Instructions does not guarantee user safety. It is the responsibility of the user’s employer to ensure safe operation and training. Training courses are available and can be arranged by contacting your local GE Healthcare representative. Information given in this Operating Instructions is suggested best working practice and shall in no way take precedence over individual responsibilities or local regulations.
  • Page 11 Ensure that there is no pressure in the column before disconnecting any tubing. CAUTION Ensure that all tubing and hoses are placed so that the risk for tripping accidents is minimized. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 12 NOTICE Ensure that the pressure not exceed the specified operating pres- sure of the medium packed in the column. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 13 Inspect the column regularly for signs of corrosion, which may cause column damage if untreated. NOTICE Keep a visual check on the adapter when it is moving, to monitor smooth movement. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 14: Column Pressure And Over-Pressure

    Section 3.2 Primary specifications, on page 22 presents the maximum pressure that may be used for packing and operation procedures using AxiChrom 50, 70 and 100. For safe use of the columns, these pressure limits shall be observed. Particular attention shall be taken when connecting manual pumps to the column, or deviation in normal operating procedures using ÄKTAexplorer or ÄKTApilot that can lead to an over-pressure.

  • Page 15: Labels

    The label for serial number, code number, and manufacturing year of the specific column is located on the outer edge of the top bayonet. Figure 2.1: The AxiChrom 50, 70 and 100 label for serial number, code number, and manufacturing year.
  • Page 16 Warning! Read the user documentation before using the system. Do not open any covers or replace parts unless specifically stated in the user documentation. The system complies with applicable European directives. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 17: Specifications And Characteristics

    3 Specifications and characteristics Specifications and characteristics Principles of column function Axial compression Packing of AxiChrom 50, 70 and 100 is achieved by internal hydraulic axial compression (Figure 3.1). Check valve prevents backflow Packing liquid Packing liquid Packing liquid forced out...
  • Page 18 (usually a few hours up to 48 hours depending on the type of medium), where the packed bed height is read when the bed has been mechanically compressed to its final condition for use. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 19 (shown in picture), and the 70 and 100 columns with the AxiChrom pivot stand (not shown here). Part Function Top mobile phase inlet/outlet Vent valve, Hydraulic chamber (hidden behind adaptor rod) Unpacking (Hydraulic chamber outlet) Hydraulic chamber inlet Bottom mobile phase inlet/outlet AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 20 3.0 mm 3.5 mm 4.0 mm Hydraulic chamber vent valve, M8 0.9 mm 0.9 mm 0.9 mm Dependant upon media e.g.: 1.7 mm for Sepharose HP, 2.9mm for Capto media. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 21 Compression occurs according to a pre- determined Packing Factor. Compression of the bed causes an increase in the pressure (X). Following compression the target Packing Factor is attained (C). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 22: Primary Specifications

    350 x 360 (pivot stand) 350 x 360 (pivot stand) Refers to the maximum allowed pressure for the column, and specifically the maximum pressure in the hydraulic chamber during the packing procedure. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 23: Column Lengths And Bed Support Materials

    AxiChrom column foot for the 50 column and pivot stand for the 70 and 100 columns. Column lengths and bed support materials AxiChrom 50, 70 and 100 are each available in two column tube lengths. Maximum bed height for the short column tube is 300 mm, and for the long column tube 500 mm. The columns are listed below: •...
  • Page 24 EPDM, FKM/FPM and UHMWPE Adapter (50 and 70 columns) Stainless steel EN1.4404 (316L), EN1.4462 (Duplex) Bottom plate (50 and 70 columns) Stainless steel EN1.4404 (316L), EN1.4462 (Duplex) Support net (50 and 70 columns) AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 25 (316L) and 1.4435 (316L) may also have been used. All these are to standards EN 10028-7 and EN 10272. For components made of Stainless steel EN1.4401, stainless steel EN 1.4436 (316L) may also have been used. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 26: Chemical Resistance

    3.5 Chemical resistance Chemical resistance Table 3.3 lists the chemical resistance of AxiChrom 50, 70 and 100 to various chemicals. The concentrations listed should not be exceeded during an operating cycle. In general, avoid using the following chemicals in AxiChrom columns: •...
  • Page 27 Chemical Safety. Refer to Material safety data Sheet (MSDS) for more information. pH in these solutions depends on the pH of the liquid, which can vary between pH 3-13. Glass and stainless steel columns. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 28 It is also recommended to empty the hydraulic chamber before storage of a packed column. Use a mechanical locking equipment to keep the axial compression (see Me- chanical locking, on page 39). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 29: Packing Flow Rates

    This is due to the adapter rod volume in the hydraulic chamber. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 30: Getting Started

    Column tube assembly [B] with bottom plate assembly [C] mounted • Adapter and top plate assembly [A] Additionally the following components are delivered: • AxiChrom foot with AxiChrom 50 column (pivot stand available as an accessory), Pivot stand with AxiChrom 70 or 100 • Bayonet tool (x2) •...

  • Page 31: Identifying The Main Components Of The Column

    4 Getting started 4.1 Checking the packing list Check the equipment for obvious signs of damage that may have occurred during transportation. If there is any damage please contact your local GE Healthcare represen- tative. Identifying the main components of the column Figure 4.1: Main components of the column;...

  • Page 32: Bed Supports

    µm and 20 µm. Top and bottom bed supports, adapters and bottom plates for 10 and 20 µm porosity are not physically interchangeable for AxiChrom 50 and 70 columns with stainless steel bed supports. Instead, all four column parts must be changed in order to change porosity.
  • Page 33 4 Getting started 4.3 Bed supports Bed support material GE Healthcare provides two different bed support materials, stainless steel and polyethylene, for AxiChrom columns. Stainless steel bed support dependent column parts: Applies to all column sizes unless otherwise stated. Pos no...

  • Page 34: Wetting Instructions For Plastic Bed Supports

    3 Place the wetted bottom bed support in a new snap ring. Fit the bed support and snap ring assembly onto the bottom distributor and mount on to the column as de- scribed in this Operating Instruction. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 35: Other Equipment

    Syringe for air purging of the hydraulic chamber. Equipment needed for Intelligent Packing The following equipment is needed to perform Intelligent Packing: • Computer with UNICORN 5.01 or higher including Intelligent Packing strategy • Compatible ÄKTA system AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 36: Routine Procedures

    4 Getting started 4.5 Other equipment Table 4.1: System and column dependence for AxiChrom 50, 70 and 100. ÄKTA system Column diameter (mm) ÄKTApilot 50, 70 and 100 ÄKTAexplorer ÄKTA avant 150 50 and 70 Equipment needed for manual packing If AxiChrom column is to be manually packed it is important to select a pump that can adequately supply the necessary flow rate.
  • Page 37 Then guide the two holes at the top bayonet onto the pins at the upper end. 4 Secure the column top at the stand arm with the screws [701-2]. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 38 3 Secure the arm again by first pushing and then screwing the pin into the stand arm. NOTICE The adapter and top plate assembly must be removed prior to releasing the pin at the stand for tilting the column. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 39 Figure 4.2: Releasing the pin at the stand (1) to be able to tilt the column (2). Fitting a 50 column onto the AxiChrom foot AxiChrom 50 column is delivered with an AxiChrom foot [123-1]. Place it in the foot on an even and level foundation and tighten the screws [123-2].
  • Page 40 If the hydraulic chamber vent valve is opened while there is pressure in the hydraulic chamber, liquid will spray out. Take necessary precautions to avoid personal injury or damage to equipment. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 41 1 Prime the adapter and bottom bed supports (see Priming the column, on page 73). 2 Fill the column with an appropriate liquid or slurry up to the start of the glass incline of the column tube. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 42 It is important that the adapter rests on the chamfering to be certain that the adapter starts to move downwards when pumping liquid into the hydraulic chamber. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 43 To avoid use of excessive force and risk of trapping fingers, lu- bricate the adapter rod with 20% (v/v) ethanol to make sure that the top plate can move smoothly. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 44 [804]. 7 Insert one of the bayonet tools into the hole on the top plate, and the other tool into the hole on the top bayonet (see Figure 4.5). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 45 Figure 4.5: Opening and closing of the bayonet. WARNING Ensure that the top plate is completely closed by confirming that the tool in the top plate is aligned with the machined mark on the top bayonet. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 46 1 Ensure that the top mobile phase inlet/outlet and the hydraulic vent valve are closed. 2 Attach one end of a tubing to the hydraulic chamber outlet and the other end of the tubing to waste. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 47 Beware when handling the sharp edges of the bayonet threads. NOTICE Never force the adapter out from the column manually. Only use hydraulic pressure as described in this Operating Instruc- tions to move the adapter in the column tube. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 48 CAUTION Risk of leakage. Take proper precautions for personal protection and surrounding equipment. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 49 8 Carefully place the top plate and adapter on a flat surface. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 50: Over-Pressure Situation

    The mechanical locking equipment is attached to the • column while trying to move the adapter upwards Mechanical error System runs towards a closed valve Reliability error A system error occurs in the monitoring system AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 51 If the hydraulic chamber vent valve is opened while having pressure in the hydraulic chamber liquid will spray out. Take necessary pre- cautions to avoid personal injury or damage on equipment. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 52 Do not touch the syringe plunger during this step. 3 If the syringe fills up before the pressure has normalized, close the valve, discard the contents of the syringe and repeat from step 1. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 53: Preparing The Column For A Run

    Take care not to subject the adapter rod to mechanical forces as this could lead to malfunctioning of the column. CAUTION Use protective footwear whenever the column is moved to avoid injury. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 54 For correct positioning of the centering tool, see Figure 6.26. After moving the column to its resting position, level the column (see below). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 55 4.8 Preparing the column for a run Moving the 50 column with an AxiChrom foot An AxiChrom 50 column fitted into the foot can be manually lifted and moved. If neces- sary, the column can be placed onto a trolley or other stable transport equipment. The column shall be lifted vertically using the top bayonet and a hand supporting the column foot.
  • Page 56 With exception to plastic parts, all parts of AxiChrom 50, 70 and 100 may be autoclaved if required. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 57: Installation In Basic Configuration

    For details about tubing, see section Tubing used between column and system, on page 60. Column Valve V2 Column Valve V3 Figure 4.8: Basic configuration for connecting AxiChrom columns to ÄKTAexplorer system. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 58 Packing phase and according to those column valves chosen in the method, see step 6 in section Creating an Intelligent Packing method using GE approved media, on page 84. For details about tubing, see section Tubing used between column and system, on page 60.
  • Page 59 1.7 / L=800 (x1) id. 1.7 / L=1200 (x1) id. 2.9 / L=1600 (x1) id. 2.9 / L=1600 (x1) id. 1.7 / L=200 (x2) id. 1.7 / L=200 (x2) AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 60: Installing The Unicorn Intelligent Packing Strategy On Äktaexplorer And Äktapilot

    CD supplied with the column. The latest Wizard can be downloaded from the AxiChrom web page, see Sec- tion 8.2 Contact information, on page 155. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 61: Selecting The Intelligent Packing Strategy And Component

    5 Ensure that the check box for the component IntelligentPacking is checked. Figure 4.10: Component dialog in UNICORN. 6 Click on OK in Component and Edit System dialogs. For more details on how to install components, see UNICORN Online Help. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 62: Operation

    The adapter rod attachment to the adapter may loosen after a period of use. The tightness shall be checked at regular intervals and be retightened when appropriate (Servicing the adapter rod, adapter tubing and top plate (stainless steel bed support), on page 119). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 63: Intelligent Packing Of The Column With Äktaexplorer And Äktapilot

    Intelligent Packing allows for convenient and precise control of the column packing using AxiChrom column together with ÄKTA system, UNICORN software, and verified packing methods. Several BioProcess™ media are available from GE Healthcare that have been verified for use with AxiChrom column and Intelligent Packing. These are found in a list of media in the method wizard for Intelligent Packing.
  • Page 64 Note: All S and SP ligand media are shipped and supplied in 0.2 M sodium acetate and 20% ethanol. Any decanted solution will contain salt. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 65 Note: It is recommended to click the Set Default button before a new method is created for Intelligent Packing. This will make sure that the default packing parameters are used. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 66 Show Select the appropriate media type. For custom media see Creating medium type an Intelligent Packing method based on custom media, on page 71. Medium Select the appropriate BioProcess medium. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 67 There are different scales for stainless steel and plastic bed supports on the column. Inlet for hydraulic chamber Select the appropriate inlet. liquid Inlet for mobile phase Select the appropriate inlet. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 68 Over-compression during packing can damage the medium. In the Intelligent Packing tests performed by GE Healthcare on its base media selection, the default Packing Factor for each medium has been used and verified. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 69 Leave checked to use the suggested default liquid during packing test velocity of 30 cm/h. Packing test upflow and Select whether to perform packing tests upflow, Packing test downflow downflow or both. Both are selected by default. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 70 This page provides a summary of the options selected in the previous wizard pages. A “Suggested slurry recipe” and estimated buffer consumption are also presented. The summary page gives a reminder that it is important to change to the recom- mended packing liquid. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 71 1 On the Main page of the wizard click on the Special Method radio button, and then select Intelligent Packing from the list. For ÄKTAexplorer, choose Intelligent Packing in the Main Selection scrollbar. 2 Select the appropriate AxiChrom column. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 72 Over-compression during packing can damage the medium. In the Intelligent Packing tests performed by GE Healthcare on its base media selection, the default Packing Factor for each medium has been used and verified. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 73 6 Place the adapter (bed support downwards) into a container of liquid to prevent air coming into the bed support, adapter and tubing. Note: The adapter of the AxiChrom 50 column may need extra support to stand in the container of liquid, for example by leaning it against the wall or an- other surface.
  • Page 74 7 Fit the adapter and top plate onto the column (see Fitting the top plate and adapter, on page 41). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 75 Ensure that the full flow path is unrestricted when pumping liquid into the column. 4 Start the Intelligent Packing method and follow the instructions: a ÄKTA system performs a wash procedure to fill the system with hydraulic liquid. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 76 (Creating an Intelligent Packing method using BioProcess media, on page 65) and can be viewed in the method notes. Before the consolidated bed height is reached a warning dialog is displayed. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 77 Packing Factor respectively. It is also important to correctly judge the correct level of the bed surface since medium can sometimes accumulate at the edges of the consolidated bed. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 78 6 It is recommended to replace the tubing with a stop plug [101] at the check valve [116]. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 79: Intelligent Packing Of The Column With Äkta Avant 150

    Intelligent Packing allows for convenient and precise control of the column packing using AxiChrom column together with ÄKTA system, UNICORN software, and verified packing methods. Several BioProcess™ media are available from GE Healthcare that have been verified for use with AxiChrom column and Intelligent Packing. These are found in a list of media in the Intelligent packing phase.

  • Page 80: Create A Column Type

    Open the Column Handling dialog To open the Column Handling dialog: • select Tools:Column Handling... in any of the UNICORN modules • click the Column Handling icon in the Toolbar where available AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 81 In the Column Type Parameters tab in the Column Handling dialog, click Result: The New Column Type dialog opens. Note: The Intelligent Packing method is only compatible with AxiChrom columns. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 82 To filter the drop-down list to only show hardware types with certain diameters, enter the diameter range in cm in the Min and Max fields for Show hardware types by diameter (cm) above. Select the GE Healthcare medium type for the new column type in the • drop-down list.
  • Page 83 Result: The column type is saved in the database and displayed in the Column types list. Note: If traceability of result is requested, a column individual can be created under column type for logging of the data, see UNICORN 6.1 Method Manual. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 84: Creating An Intelligent Packing Method Using Äkta Avant 150

    This section describes the creation of an example Intelligent Packing method based on GE approved media. If custom media is selected instead, the method settings allow a wider range of variables to be defined to optimize the method. Follow the instruction below to create an Intelligent Packing method based on GE approved media.
  • Page 85 Result: The phases included in the chosen method are displayed in the Method Outline pane to the left, and the default settings for each of the phases are shown in the Phase Properties pane to the right. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 86 Make sure that all parameter values are correct. Note: If the check box Column Performance Test is checked, automatic logging of the result will be saved in the column logbook if a column individual is chosen. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 87 In the Phase Properties pane of the Intelligent Packing phase, make sure that the GE approved packing settings radio button is clicked. This button should be clicked if an AxiChrom column type with GE approved media was chosen in the Method Settings phase. Default parameter values for packing will automatically be filled in into the Intelligent Packing phase.
  • Page 88 Result: The Suggested Slurry Recipe dialog opens. Note: The Slurry Recipe button is not available in Custom Packing settings. Click OK to close the dialog. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 89 Result: The How to connect the column to the system dialog opens. Note: The text will be updated dependent on the column positions chosen, but the illustration will remain the same. Click OK to close the dialog. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 90 The illustration below shows the Method Outline pane to the left and the Phase Properties pane of the Equilibration phase to the right. Note: All parameters in this phase can be changed. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 91 At both tests the flow rate is set to 30 cm/h. It is important to use a low flow and to use the same flow rate if tests are repeated to get comparable results. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 92 Specify desired adapter velocity during packing within defined range In the Phase Properties pane of the Intelligent Packing phase, make sure that the Custom packing settings radio button is clicked. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 93: Preparing The Slurry, Priming And Packing The Column Using Äktaavant

    5.2.2 Creating an Intelligent Packing method using ÄKTA avant 150 The illustration below shows the Method Outline pane and the Phase Properties pane of the Intelligent Packing phase. Make the appropriate changes and save the method in the same way as for GE approved packing. 5.2.3 Preparing the slurry, priming and packing the column using ÄKTAavant...

  • Page 94: Manual Packing Of The Column

    3 Connect the AxiChrom column to a compatible ÄKTA system (see Section 4.9 Instal- lation in basic configuration, on page 57). 4 Prime the column (see Priming the column, on page 73). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 95 7 Place a syringe (without plunger) onto the hydraulic chamber vent valve, and open the vent valve. 8 To pump liquid into the hydraulic chamber: For ÄKTApilot ÄKTApilot: in the UNICORN System Control module choose Manual in the menu and Flowpath:Columns :Col1_Col2_Packing. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 96 Continue to compress until the target Packing Factor (PF) is reached. consolidated bed height packed bed height Read the bed height. Calculate the achieved Packing Factor (PF). 13 Turn off the pump when the target bed height is reached. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 97 (see Section 5.4 Performance evaluation of the column, on page 100). 16 It is recommended to replace the tubing with a stop plug [101] at the check valve [116]. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 98 5.3 Manual packing of the column Manual packing with a separate packing pump It is possible to pack AxiChrom 50, 70 and 100 with a separate packing pump. CAUTION Ensure that the pivot stand locking pin is secured before starting the process so that the column cannot turn over by accident.
  • Page 99 The column is now packed. Before using it, evaluation of the packing is recommended. 15 It is recommended to replace the tubing with a stop plug [101] at the check valve [116]. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 100: Performance Evaluation Of The Column

    UV curve, or conductivity curve if sodium chloride is used as sample, as follows: HETP = L/N N=5.54 (V Variable Meaning Bed height Plate number Retention volume Peak width at half peak height and w are in the same units AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 101 10% of peak height: = b/a The following test chromatogram shows the parameters used to calculate peak broad- ening and peak symmetry. -296 -296 -296 -253 0.85 0.85 0.85 Eluted Volume AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 102: Unpacking A Packed Column

    46. A sudden pressure decrease indicates that the adapter has passed the beveling at the top of the (glass) tube. 9 Remove the top plate and adapter (Removing the top plate and adapter, on page 49). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 103: Cleaning-In-Place (Cip)

    Low liquid velocity (60 cm/h) is used for the whole process. Note: Before the sanitization method is started, check that the media withstands 1.0 M NaOH. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 104 4 hours at a velocity of 60 cm/h. 4 Wash the column with buffer or water of desired quality to achieve a neutral pH. 5 Re-equilibrate the column with storage or starting buffer. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 105: Emptying The Column

    3 Release the pin at the pivot stand, if used, tilt the column and pour out medium into a container. 4 Pump in packing liquid upflow if needed to help in pouring the medium. Figure 5.8: Emptying the column using the pivot stand. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 106: Storage

    Low pH solutions containing chlorides can cause corrosion in stainless steel. Rinse thoroughly with clean water after each use with such solutions. Inspect the column regularly for signs of cor- rosion, which will cause column damage if untreated. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 107 For plastic bed supports, ensure that the snap ring on the bed support is stored in a way that prevents mechanical damage. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 108: Maintenance And Service

    In some cases bold style characters, either a number or letter may be contained in { } brackets. These correspond to parts on the 100 column only that deviate from the commonly assigned items identified in square brackets (see above). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 109 Heavy object. At least two persons are required to lift objects over 20 kg. NOTICE If leaving the column unattended always rest it horizontally on the planar surfaces of the flanges or hanging on the pivot stand as appropriate. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 110 Note: Two are supplied but only one is needed. Hook wrench key Yes (For maintenance of Stainless steel bed sup- ports) Removal tool Yes (For maintenance of plastic bed supports) Supplied with column AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 111: Performing Maintenance And Service

    Make sure that the O-rings are checked regularly and when appropriate replaced with new ones. GE Healthcare recommends that if during maintenance and service an O-ring is removed from its position on the column, then it should always be replaced. Otherwise, it is recommended that the O-rings are replaced once a year.
  • Page 112 AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 113 Place the adapter bed support onto a protective surface, e.g., plastic film, on a flat surface. Lubricate the O-ring on the bed support with 20% (v/v) ethanol. Note: The 10 and 20 µm bed supports are not interchangeable. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 114 O-ring, which may cause leakage. Continue until the O-ring has come into contact with the top of the wall of the scraper seal [605]. Figure 6.2: Placement of the O-ring onto the bed support. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 115 O-ring onto the adapter bed support. Figure 6.4: Correct fitting of the O-ring (A) versus incorrect fitting as seen by the ballooning out of the O-ring (B). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 116 0.5 Nm for 50 and 70 columns and 1 Nm for the 100 column. Do not overtighten. When tightened, there will be a gap of approximately 1 mm between the bed support and the adapter. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 117 50 column), invert the column in the stand (Using the pivot stand, on page 38). For AxiChrom 50 column in the foot [123-1], unscrew the bolts [123-2] and lift the column out of the AxiChrom foot and place the column lying down on the flanges.
  • Page 118 [403] will cause the O-ring [607] {411b} to be pushed onto the bed support and damage the bed support. ~ 0,5 mm Note: The 10 and 20 µm bed supports are not interchangeable. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 119 [D]. Carefully use the tool to obtain increased leverage when unscrewing the adapter. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 120 Figure 6.6: Using the hook wrench key for increased leverage to unscrew the adapter from the adapter rod. 3 Gently pull on the adapter to withdraw the tubing from inside the adapter rod. Figure 6.7: Withdrawing the tubing from inside the adapter rod. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 121 6 Slide the top plate [803] down the length of the adapter rod [802] and remove. Apply a small amount of ethanol 20% (v/v) on the adapter rod for easier movement of the top plate along the adapter rod. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 122 3 Slide the top plate onto the adapter rod from the threaded end. Apply a small amount of ethanol 20% (v/v) on the adapter rod for easier movement of the top plate along the adapter rod. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 123 This may cause leaks inside the adapter rod resulting from the adapter tubing coming loose. Figure 6.10: Tightening the stop screws. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 124 It is recommended that the stop screws are not fully removed from the adapter rod top to help avoid losing them. 2 Use an Allen key to remove the screws [401], washers [412] and O-rings [413] from the adapter [501]. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 125 8 Rinse the distributor with water and then inspect for signs of damage. If necessary replace the distributor. 9 Check the adapter plate O-ring [127] for visible signs of damage. If damaged replace it with a new one. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 126 O-ring and to avoid trapping air. NOTICE Avoid rolling the O-ring down the distributor as this may lead to twisting of the O-ring, which may cause leakage. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 127 O-ring circumference. Continue until the O-ring is located within the groove. Figure 6.16: Location of the O-ring into the groove of the scraper seal. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 128 1 Nm for the 100 column and to 0.5Nm for the 50 and 70 column. When tightened, there will be a gap of approximately 1 mm between the bed support and the adapter. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 129 Note: Check that the snap ring is correctly and evenly mounted around the whole circumference of the distributor. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 130 Check that the snap ring is correctly and evenly mounted around the whole circumference of the distributor. Figure 6.20: Fitting the bed support in the snap ring and the snap ring onto the distributor (100 column). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 131 50 column), invert the column in the stand (see Using the pivot stand, on page 38). For AxiChrom 50 column in the foot [123-1], unscrew the bolts [123-2] and lift the column out of the AxiChrom foot and place the column lying down on the flanges.
  • Page 132 Ensure that the bed support is in contact with the distributor all around the circumference. Note: Check that the snap ring is correctly and evenly mounted around the whole circumference of the distributor. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 133 NOTICE Do not physically push the bottom plate assembly into the column. Carefully place it so that the holes on the circumference are aligned and tighten the bolts [801] crosswise. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 134 The O-rings in the top plate are difficult to remove and should only be re- placed if part of an annual service, or in case of visible damage, or suspect- ed leakage. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 135 Note: The parts can loosen after using the column several times. Check that the adapter rod is tight before performing each packing procedure. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 136 Fit in a new O-ring in the groove in the top and bottom, making sure to press it in to the groove at all points along its circumference. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 137 Figure 6.25: Tilting the column. Hold the column at the centering tool and one of the flanges (A), and lay the column on its planar side (B). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 138 If they cannot be moved the column tube may not be properly aligned. If so, undo the nuts [806] and gently twist the top and bottom to improve the alignment. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 139 This flattened end should be used to screw into the top bayonet. 3 Repeat for other tie bars as appropriate. 4 Continue reassembly of the column (see Servicing the column tube assembly, on page 136). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 140 4 Fit in a new check valve [118]. The arrow on the check valve represents the flow di- rection and shall be fitted so that it points inwards to the column. Figure 6.27: Positioning of the check valve on AxiChrom 50 or 70 column. CAUTION...
  • Page 141 4 Screw the check valve adapter back into the top bayonet. 5 Screw the check valve housing back into the check valve adapter. Be careful not to over tighten. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 142: Performing A Drainage Test

    (see Servicing the plastic adapter bed support, tubing and rod top , on page 124). Note: Make sure that the exact same tubings are used in the drainage test since this can contribute to the pressure drop and thereby the drainage rate. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 143: Performing A Column Leakage Test

    (Flow = 0). If Pause or Stop are used to stop the flow, this will cause the registration of pressure to cease. 11 Wait about 15 minutes until pressure is stable. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 144 Complete the test, empty the column, and take the necessary steps to rectify the suspected problem. Repeat the leakage test. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 145: Troubleshooting

    For stainless steel bed support: Disas- semble the column and remove the medium using ultrasound or com- pressed air, or replace the bed support. For plastic bed support: Discard the clogged PE bed supports. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 146 0.4 M salt liquid. plates too low). conditions used, e.g. NaCl for ion exchange chromatogra- phy, or chromatography medium in water as running liquid. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 147 For example, if the inner diame- ters of the tubing, valves, connectors, etc. are wider than the column inlet and outlet, change them to the same diame- ter. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 148 No elution peaks Too diluted sample solution Make sure that the correct sample with • observed. or wrong sample solution. the correct concentration is used. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 149: Adapter

    Then repack the column. The scraper seal might be damaged. • Change the scraper seal. (See Servicing the stainless steel adapter bed support, on page 112.) AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 150 Check that the pump used for packing • delivers the correct volume per time. Unpack column and perform column • leakage test (see Section 6.4 Performing a column leakage test, on page 143). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 151 • scraper seal and the O-ring is restart the packing procedure. pushed out due to Remount the scraper under water. • compression of the O-ring when the adapter is lowered. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 152: Top Plate

    This specified. is to avoid siphoning flow. Centering tool fas- Can occur during transporta- Place the column in a cold-room over • tens. tion night. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 153: Ordering Information

    Tubing kit AxiChrom 50 / PVDF 28-9888-89 ÄKTAavant / desk Tubing kit AxiChrom 70 / PVDF 28-9888-92 ÄKTAavant / floor Tubing kit AxiChrom 50-70, PVDF 28-9136-13 i.d. 1.7 mm/ ÄKTApilot / floor AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 154 Stop plug, domed nut M6 18-2450-01 Stop plug 5/16 PEEK 28-9819-57 Bed support kit (SS) AxiChrom 50, See individual 28-9664-49 10 µm (bed support top, bottom, parts adapter, bottom plate etc.) AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 155: Contact Information

    See Product documentation spare parts list in binder. Information is also available on: www.gelifesciences.com/axichrom. Contact information For further information, please contact your local GE Healthcare representative. Infor- mation is also available on: www.gelifesciences.com/axichrom. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 156: A Parts List And Diagrams Axichrom 50 Column

    Check Valve Washer 6.4x12 123-1 AxiChrom foot 50 123-2 Hex. socket round head screw M6x30 O-ring 3x1 131 a,b Stop plug 5/16" USPVI Glass tube 50/300, Short Glass tube 50/500, Long AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 157 Adapter 50 Bottom plate 50 602 a,b O-ring 3x1 O-ring 44.12x2.62 Scraper 50 O-ring 44.2x3 FPM Screw M4x16 SS Adapter rod 50 Top plate 50 Bottom flange 50 Hex. nut M6 AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 158 Centering tool 50 Diagrams Symbol Meaning Number corresponds to Pos. no. in Material conformity and Spare part list. Illustrating parts in contact with process liquids and/or that are pressure retaining. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 159 A Parts list and diagrams AxiChrom 50 column Figure A.1: Column and foot. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 160 A Parts list and diagrams AxiChrom 50 column Figure A.2: Column tube, adapter and bottom plate. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 161 A Parts list and diagrams AxiChrom 50 column Figure A.3: Adapter assembly (stainless steel bed support). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 162 A Parts list and diagrams AxiChrom 50 column Figure A.4: Adapter assembly (plastic bed support). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 163 A Parts list and diagrams AxiChrom 50 column Figure A.5: Adapter plate assembly and bottom plate assembly (stainless steel bed support). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 164 A Parts list and diagrams AxiChrom 50 column Figure A.6: Adapter plate assembly and bottom plate assembly (plastic bed support). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 165 A Parts list and diagrams AxiChrom 50 column Figure A.7: Column tube assembly. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 166: Parts List And Diagrams Axichrom 70 Column

    117 a,b O-ring 3.6x1.6 Check Valve Washer 6.4x12 O-ring 3x1 131 a,b Stop plug 5/16" USPVI Glass tube 70/300 Glass tube 70/500 Bed support 70 top Bed support 70 bottom AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 167 Scraper 70 O-ring 63.09x3.53 701-1 Pivot stand 50/70/100-300 701-1 Pivot stand 50/70/100-500 701-2 Screw M6x12 Screw M4x16 SS Adapter rod 70 Top plate 70 Top bayonet 70 Bottom flange 70 AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 168 Centering tool 70 Diagrams Symbol Meaning Number corresponds to Pos. no. in Material conformity and Spare part list. Illustrating parts in contact with process liquids and/or that are pressure retaining. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 169 B Parts list and diagrams AxiChrom 70 column Figure B.1: Column and pivot stand. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 170 B Parts list and diagrams AxiChrom 70 column Figure B.2: Column tube, adapter and bottom plate. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 171 B Parts list and diagrams AxiChrom 70 column Figure B.3: Adapter assembly (stainless steel bed support). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 172 B Parts list and diagrams AxiChrom 70 column Figure B.4: Adapter assembly (plastic bed support). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 173 B Parts list and diagrams AxiChrom 70 column Figure B.5: Adapter plate assembly and bottom plate assembly (stainless steel bed support). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 174 B Parts list and diagrams AxiChrom 70 column Figure B.6: Adapter plate assembly and bottom plate assembly (plastic bed support). AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 175 B Parts list and diagrams AxiChrom 70 column Figure B.7: Column tube assembly. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 176: C Parts Lists And Diagrams Axichrom 100 Column

    115 a,b Tube support 100 Check valve holder Check valve, single cpl Washer 8.4x16x1.5 131 a,b Stop plug 5/16" USPVI Check valve adapter O-ring 70x2.5 Glass tube 100/300 Glass tube 100/500 AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 177 Bottom plate 100, SS bed support Bottom plate 100, plastic bed support O-ring 3x1 602 a,b O-ring 3x1 O-ring 91.67x3.53 Scraper 100 O-ring 88x4 701-1 Pivot stand 50/70/100-300 701-1 Pivot stand 50/70/100-500 AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 178 Hook wrench key Diagrams Symbol Meaning Number corresponds to Pos. no. in Material conformity and Spare part list. Illustrating parts in contact with process liquids and/or that are pressure retaining. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 179 C Parts lists and diagrams AxiChrom 100 column 131a 701-2 131b 701-1 701-3 701-4 Figure C.1: Column and pivot stand. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 180 C Parts lists and diagrams AxiChrom 100 column Figure C.2: Column tube, adapter and bottom plate. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 181 C Parts lists and diagrams AxiChrom 100 column 602a 405-3 405-1 101a 405-2 602b Figure C.3: Adapter assembly, stainless steel bed support. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 182 C Parts lists and diagrams AxiChrom 100 column 602a 405-3 405-1 101a 405-2 602b Figure C.4: Adapter assembly, plastic bed support. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 183 C Parts lists and diagrams AxiChrom 100 column 411a 411b Figure C.5: Adapter plate assembly and bottom plate assembly, stainless steel bed support. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 184 C Parts lists and diagrams AxiChrom 100 column 421a 421b Figure C.6: Adapter plate assembly and bottom plate assembly, plastic bed support. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 185 C Parts lists and diagrams AxiChrom 100 column 101b 114a 115a 115b 114b Figure C.7: Column tube assembly. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 186: D Tubing Kits And Connections

    M6 female, both valve ends 18-1169-16 Connection M6-5/16 M6 female - 5/16" male 03-0072-91 O-ring 3 × 1 To be mounted at tubing ends Optional, not included in the tubing kits. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 187 D Tubing kits and connections Figure D.1: Kits and connections for ÄKTAexplorer. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 188 For 70 columns, it is recommended to use the 70 - 100 tubing for media with an approximate bead size > 50µm. Optional, not included in the tubing kits. The SRV-1 and 5/16 straight valves are good alternatives to stop plugs when the column is moved frequently. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 189 D Tubing kits and connections Figure D.2: Kits and connections for ÄKTApilot. AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...

  • Page 190: Index

    Column tube assembly, 137 let, 31 Compression of consolidated Bottom plate bed, 76 Bed support cleaning, 117 Consolidated bed Compression, 76 Check valve servicing, 140, 141 CIP, 103 Drainage test, 142 Cleaning, 56 AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 191 Slurry volume factor, SVF, 64 ment, 39 Spare parts Fitting, 39 AxiChrom 70 column, 166 Removing, 41 AxiChrom 100 column, 176 Moving the column, 55 Recommended to keep on- site, 155 AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 192 Top mobile phase inlet/out- let, 31 Wizard Top plate Slurry concentration, 69 Fitting, 41 Wizard for Intelligent Pack- Removing, 49 ing, 65 Top plate servicing, 119, 124 Custom media, 71 AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG...
  • Page 194 All goods and services are sold subject to the terms and conditions of sale of Sweden the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare repre- www.gelifesciences.com/axichrom...

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