Thermo Scientific LCQ Fleet Getting Started Manual page 54

6
Connecting the Inlet Plumbing
Sample Introduction
Table 8. Sample introduction and analytical techniques for ESI/MS and APCI/MS
Sample introduction into
the MS detector
Direct infusion
High-flow infusion
(syringe pump injection into
LC solvent flow)
Loop injection into LC solvent
flow
LC system with autosampler
(without chromatographic
separation)
LC system with autosampler
(with LC column for
chromatographic separations)
40 LTQ Series Getting Connected Guide
• For automated injections using an autosampler, connect the outlet from a liquid
chromatography system that contains an autosampler to port 2 of the divert/inject valve.
Then set up an autosampler to make automated injections into the solvent flow produced
by the LC pump.
Table 8 summarizes the sample introduction and analytical techniques for ESI/MS and
APCI/MS.
ESI analytical
technique
Analysis of a pure
analyte
Automatic calibration
and tuning
Analysis of a pure
analyte
Analysis of a pure
analyte
Automatic
optimization of tuning
using an analyte
Analysis of one or more
pure analytes
Analysis of a mixture
APCI analytical
technique
Analysis of a pure
analyte
Analysis of a pure
analyte
Automatic
optimization of tuning
using an analyte
Analysis of one or more
pure analytes
Analysis of a mixture
Procedure for connecting the
plumbing
"Setting Up the Inlet for Direct
Infusion" on page 43
"Setting Up the Inlet for
High-Flow Infusion" on
page 45
"Setting Up the Inlet for Loop
Injections (Flow Injection
Analyses)" on page 51
"Setting Up the Inlet for an
LC/MS System with an
Autosampler" on page 53
"Setting Up the Inlet for an
LC/MS System with an
Autosampler" on page 53
Thermo Scientific