Description; Introduction; Instrument Function - Beckman Coulter Optima L-XP Instructions For Use Manual

Introduction

This section describes the Optima L-XP series ultracentrifuge components and their functions. It also
describes system safety features and centrifuge controls and displays. Refer to the applicable rotor manual
for rotor descriptions.

Instrument Function

The Optima L-XP ultracentrifuge is used to generate centrifugal forces for the separation of
particles. The Optima L-100 XP has a maximum rotational speed of 100,000 RPM; the L-80 XP runs
up to 80,000 RPM. Classified S, it can be used with all currently manufactured Beckman Coulter
preparative rotors.
The microprocessor-controlled Optima L-XP provides an interactive operator interface, using a
touchscreen and keypad, with the eXPert operating software.
Both manual and programmed operation are available. In manual operation, you enter the
individual run parameters and begin the run. In programmed operation, you can create, save,
recall, modify, and/or print a program, and then automatically run the ultracentrifuge via the
program. Several advanced capabilities simplify laboratory ultracentrifugation, such as:
• Setting up a run by specifying RCF (relative centrifugal force), w
effect), substitute rotor and tube combination, or sedimentation coefficient
• Performing various centrifugation-related calculations, including pelleting time,
concentration measures, refractive index, speed reduction, and sedimentation coefficient
• ESP (Efficient Sedimentation Program), a simulation that provides optimum run times using
precipitating and non-precipitating media
• Rotor and tube catalogs containing pertinent information about Beckman Coulter
ultracentrifuge rotors and tubes
• A rotor library for tracking the rotors in your lab
• Run logs for record-keeping or lab procedures purposes
• Information on compatibility between centrifugation accessories and a variety of chemicals
PN LXP-IM-7AB
CHAPTER 1

Description

2
t (accumulated centrifugal
1-1