Solvents - Agilent Technologies Cary 8454 Installation Manual

Uv-visible spectroscopy system
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Solvents

Your choice of solvents should be based primarily on the solvent's
absorbance characteristics over the wavelengths of interest, its suitability
as a solvent for the analyte, and on experimental conditions.
common solvents and the lower limit of their useful wavelength range.
Table 1
Lower Limit
180–195 nm
200–210 nm
210–220 nm
245–260 nm
265–275 nm
280–290 nm
Above 300 nm
Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Lower limit of UV transmission for some common solvents
Solvent
Sulfuric acid (96%)
Water
Acetonitrile
Cyclopentane
n-Hexane
Glycerol
2,2,4-Trimethylpentane
Methanol
n-Butyl alcohol
Isopropyl alcohol
Cyclohexane
Ethyl ether
Chloroform
Ethyl acetate
Methyl formate
Carbon tetrachloride
Dimethyl sulfoxide
Dimethyl formamide
Acetic acid
Benzene
Toluene
m-Xylene
Pyridine
Acetone
Carbon disulfide
Good Measurement Practices
General Considerations
Table 1
3
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