Use Of Solvents - Agilent Technologies 1260 Infinity User Manual

Use of Solvents

Observe the following recommendations on the use of solvents.
• Brown glass ware can avoid growth of algae.
• Small particles can permanently block capillaries and valves. Therefore
• Avoid the use of the following steel-corrosive solvents:
Agilent 1260 Infinity TCC User Manual
always filter solvents through 0.4 µm filters.
•
Solutions of alkali halides and their respective acids (for example,
lithium iodide, potassium chloride, and so on),
•
High concentrations of inorganic acids like sulfuric acid and nitric acid,
especially at higher temperatures (if your chromatography method
allows, replace by phosphoric acid or phosphate buffer which are less
corrosive against stainless steel),
•
Halogenated solvents or mixtures which form radicals and/or acids, for
example:
→ 2COCl
2CHCl + O
3 2
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the
stabilizing alcohol,
•
Chromatographic grade ethers, which can contain peroxides (for
example, THF, dioxane, di-isopropylether) such ethers should be filtered
through dry aluminium oxide which adsorbs the peroxides,
• Solvents containing strong complexing agents (e.g. EDTA),
• Mixtures of carbon tetrachloride with 2-propanol or THF.
+ 2HCl
2
11
Appendix

Use of Solvents

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