Changing Solvents - Agilent Technologies 1260 Infinity VL User Manual

Changing Solvents

When When the solvent of a channel is to be replaced by another solvent that is not compatible (solvents
are immiscible or one solvent contains a buffer), it is necessary to follow the procedure below to
prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system.
Parts required #
1
1
Preparations Remove the column and replace it by a ZDV fitting.
Prepare bottles with appropriate intermediate solvents (see
1 If the channel is not filled with buffer, proceed to step 4.
2 Place the solvent intake filter into a bottle of water.
3 Flush the channel at a flow rate suitable for the installed tubing (typically
4 Modify the flow path of your system as required for your application. For
Buffer salt of aqueous buffers may precipitate in residual isopropanol.
C A U T I O N
Capillaries and filter may be clogged by precipitating salt.
➔
➔
5 Replace the solvent bottle by a bottle of isopropanol.
6 Flush the channel at a flow rate suitable for the installed tubing (typically
7 Swap the bottle of isopropanol with a bottle of solvent for your application.
8 Repeat steps 1 to 7 for the other channel(s) of the pump.
9 Install the desired column, set the required composition and flow rate for
1260 Infinity QuatPump VL User Manual
p/n Description
Purging solvent(s), see
5022-2184 Union ZDV
3 – 5 mL/min) for 10 min.
delay volume optimization, see the Rapid Resolution System manual.
Flush solvent lines containing high concentration of salts first with water before
introducing organic solvent.
Do not perform steps 5 to 7 for channels running with aqueous buffer as solvent.
3 – 5 mL/min) for 5 min.
your application and equilibrate the system for approx. 10 minutes prior to
starting a run.
Table 4 on page 46
Table 4 on page 46)
Installing the Pump
Priming the System
3
45