Agilent Technologies Cary 8454 Installation Manual
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Agilent Technologies Cary 8454 Installation Manual

Agilent Technologies Cary 8454 Installation Manual

Uv-visible spectroscopy system
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Agilent Cary 8454
UV-Visible Spectroscopy
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Summary of Contents for Agilent Technologies Cary 8454

  • Page 1 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide ...
  • Page 2 Software is subject to Agilent Tech- of Microsoft Corporation. nologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 3 Use this chapter as an overview of the different actions you must perform to install your UV- Visible spectroscopy system. If you are an experienced user of analytical equipment from Agilent Technologies, this may be all you need. If you need more information, this chapter guides you to other relevant areas in this or another handbooks.
  • Page 4 LAN Communication, Installation, Connection and Configuration This chapter describes how to set up LAN communication between your Agilent ChemStation and your UV- Visible spectrophotometer. Safety Information This chapter contains relevant safety information. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 5: Table Of Contents

    Unpacking Your Spectrophotometer Installing your UV-Visible Spectrophotometer Instrument overview Setting up the Spectrophotometer Installing Your Computer Setting up the computer Starting the Spectrophotometer Turning On Instrument Warm-up Starting the Agilent ChemStation Software Operational Checkout Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 6 Long Path Cell Holder Installing the Long Path Cell Holder Installing a Sample Cell Optical Filter Wheel Installing the Filter Wheel Assembly Removing the Filter Wheel Assembly Installing the UV-Visible Software Agilent ChemStation Computer Details Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 7 Problems with Plug and Play option GPIB cards Changing the GPIB Address of the Spectrophotometer GPIB Address Settings LAN Communication, Installation, Connection and Configuration Installing and Configuring TCP/IP on the PC Installation of the TCP/IP protocol Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 8 Spectrophotometer What is the Agilent ChemStation Configuration Editor? Configuring an Agilent Cary 8454 UV-Visible Spectrophotometer Troubleshooting LAN Communication Power failure reported on the Agilent ChemStation Frequent buffer overrun in Agilent Cary 8454 logbook Safety Information Safety Information General Operation Safety Symbols...
  • Page 9 Contents Flow Cells Solvents Lithium Batteries Information Agilent Technologies on the Internet Index Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 10 Contents Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 11: Fast Installation

    Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide Fast Installation Installing Preconfigured Agilent Cary 8454 UV-Visible Systems Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems Installing Software Only Installation is straightforward, but if you have difficulties, or need more information, references to detailed installation information are given.

  • Page 12: Installing Preconfigured Agilent Cary 8454 Uv-Visible Systems

    2 minutes for the lamps to ignite. When you are ready to operate your spectrophotometer, switch on the computer and printer. For more information, see “Starting the Spectrophotometer” on page 31. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 13: Installing Non-Preconfigured Agilent Cary 8454 Uv-Visible Spectrophotometer Systems

    Fast Installation Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems You must install and configure the software. In addition, you may need to install and configure an instrument communication interface such as the GPIB PCI interface card.
  • Page 14 Fast Installation Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems For more information, see “Installing Your Computer” on page 29. Switch on the spectrophotometer (make sure sample area is clear) and wait two minutes for the lamps to ignite. When you are ready to operate your spectrophotometer, switch on the computer and printer.

  • Page 15: Installing Software Only

    Ensure that your software and spectrophotometer operate properly, by taking a blank measurement or by performing the automatic self- test in the diagnostic section of your software. For more information, see “Operational Checkout” on page 34. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 16 Fast Installation Installing Software Only Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 17: Installation And Start Up

    Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide Installation and Start Up Site Requirements for Your Spectrophotometer Unpacking Your Spectrophotometer Installing your UV-Visible Spectrophotometer Installing Your Computer Starting the Spectrophotometer Starting the Agilent ChemStation Software Operational Checkout The following topics are covered in this section: •...
  • Page 18 There is an optional automated self- test which can be used for a quick check of the spectrophotometer. This self- test can only be executed after the instrument has warmed up for at least one hour. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 19: Site Requirements For Your Spectrophotometer

    Connecting your instrument to a line voltage that is higher than specified causes a WA R N I N G shock hazard, and damage may occur to your instrument. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 20: Power Cords

    8 cm (3.1 inches) in the rear for electrical connections. The computer, including the keyboard, is approximately 50 cm (19.7 inches) wide and 60 cm (23.6 inches) deep. Additional space is required for accessories like pumps and autosamplers. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 21: Environment

    Your spectrophotometer can be stored at altitudes up to 4,600 m (14,950 ft.) and operated at altitudes up to 2,000 m (6,500 ft.). Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 22: Unpacking Your Spectrophotometer

    Technologies. Save the shipping material for the carrier’s inspection. Check the contents of the shipping container against the list below. If any items are missing or obviously damaged, call Agilent Technologies. Unpack the spectrophotometer. You should have the following components: •...

  • Page 23: Installing Your Uv-Visible Spectrophotometer

    The spectrophotometer is shipped with the standard single- cell cell holder already in place. Standard and accessory cell holders can be removed and replaced in seconds with few or no tools. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 24 Indicator Figure 1 Front view of the Agilent Cary 8454 UV-Visible spectrophotometer The line power switch is located at the lower left part of the instrument. Pressing it in turns on the instrument. It stays pressed in and shows a green light when the instrument is turned on.
  • Page 25 GPIB connector and switches Remote connector MIO slot for LAN interface Multicell transport connector GP-IO connector Line connector RS 232 connector Figure 2 Rear view of the Agilent Cary 8454 UV-Visible spectrophotometer Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 26 • The remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down, prepare etc. • The RS 232 connector may be used to control the spectrophotometer from a computer through RS 232 connection, using appropriate software (for future use).

  • Page 27: Setting Up The Spectrophotometer

    2 Make sure the power switch of the spectrophotometer is in the off position, that is, the pushbutton stands out and the LED inside the Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 28 3 Put the other end of the waste tubing into the waste container. 4 Make sure that the waste tubing has a constant down slope from the spectrophotometer to the waste container. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 29: Installing Your Computer

    If you have more GPIB instruments connected to your computer, see Chapter 6, “Installing an GPIB Interface” for correct GPIB cabling, because you must observe some basic rules. For correct setup, see Chapter 7, “LAN Communication, Installation, Connection and Configuration”. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 30 Installation and Start Up Installing Your Computer Connect the power sockets to the computer, display and printer, and switch them on. Follow the instructions for setting up the computer in your computer documentation. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 31: Starting The Spectrophotometer

    The pushbutton will stay pressed in and light green if power is present. Indicator Line power switch with green light Figure 5 Turning on the Agilent Cary 8454 UV-Visible spectrophotometer Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 32 Because in this case there is no communication with the computer there will be no error message. The online help system and the Operator’s Manual gives more information about troubleshooting. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 33: Instrument Warm-Up

    In general, the life of your spectrophotometer will be increased by leaving it on all the time but switching off the lamps when the instrument is not in use. lamp intensity of the deuterium lamp and lifetime of lamps decrease with use. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 34: Starting The Agilent Chemstation Software

    Excessive noise or spikes may indicate a problem. 3 For the spectrophotometer an additional (optional) qualification can be automatically performed using your software. After warm- up of the spectrophotometer for one hour, the instrument is ready for the Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 35 The successful completion of these tests complete the operational qualification of your spectrophotometer. For reference purposes, you may want to print a copy of the results. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 36 Installation and Start Up Operational Checkout Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 37: Good Measurement Practices

    Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide Good Measurement Practices General Considerations Inserting a Cell This chapter describes: • making measurements • selecting material, optical specification and type of cell • handling and maintaining cells • checklist for good results •...

  • Page 38: General Considerations

    Spectrophotometer Design The sample compartment of the spectrophotometer is open. Unlike conventional instruments, the Agilent Cary 8454 UV- Visible spectrophotometer does not suffer from ambient false light. The open sample area is easier to access, and it is easier to connect tubing to a flow cell or thermostattable cell holder.

  • Page 39: Sample Cell Or Cuvette Material

    350 to 1100 nm, you can use good quality glass cells. Disposable plastic sample cells, for measurements in the range 400 - 1100 nm, are also available. The quality of these cells varies and they are generally not recommended. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 40: Optical Specifications Of Cells

    Light Beam in Parallel Cell Walls Light Beam out Light Beam in Non-parallel Cell Walls Light Beam out Figure 6 Shift of the instrument light beam due to non parallel cell walls Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 41: Apertured Cells Or Cuvettes

    Recommended cells * Quartz cells with black apertures smaller than 2 mm, when used with a multicell C A U T I O N transport, can lead to measurements of poor reproducibility. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 42: Flow Cells

    Also, the cell can be rinsed thoroughly with the solution to be measured. The design of the flow cell should minimize entrapment of bubbles and flow channeling to provide the most reliable results. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 43: Handling And Maintaining Cells Or Cuvettes

    Figure 9 Floating particles in a cell Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 44 If glass pasteur pipettes are used, make sure that the optical windows of the cell are C A U T I O N not touched or scratched by the pipette. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 45: Solvents

    Cyclohexane Ethyl ether 245–260 nm Chloroform Ethyl acetate Methyl formate 265–275 nm Carbon tetrachloride Dimethyl sulfoxide Dimethyl formamide Acetic acid 280–290 nm Benzene Toluene m-Xylene Above 300 nm Pyridine Acetone Carbon disulfide Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 46: Sample Preparation

    Samples which contain colloidal dispersions, dust or other particulate matter should be filtered, centrifuged or allowed to settle. If not, the overall attenuation- of- transmittance spectrum due to light scattering and/or reflection will hide the spectral information from the analyte. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 47: Photosensitive Samples

    To minimize convection effects keep the temperature of your sample the same as the cell holder or environmental temperature. Problems like these can also be minimized by using a thermostattable cell holder and/or a stirring module. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 48: Checklist For Best Results

    Ideally, the cell is not removed between the measurement, which means the cell is filled/rinsed using a pipette or a flow cell is used Time between blank and sample measurement should be short Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 49 Example of a blank on water with bubbles causing a poor baseline If your blank or spectra show artifacts similar to the one in Figure 12, see “Solvents” N O T E page 45 to optimize the measurement procedure. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 50: Inserting A Cell

    2 mm aperture may require use of the optional adjustable cell holder. The adjustable cell holder helps you ensure the cells are properly centered in the light path. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 51: Installing Accessories

    Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide Installing Accessories Thermostattable Cell Holder Cell Stirring Module Long Path Cell Holder Optical Filter Wheel Many simple, mechanical/optical accessories are available to expand the capabilities of your UV- Visible spectrophotometer. This section briefly describes these accessories and outlines how to install them.

  • Page 52: Thermostattable Cell Holder

    Up to 1 cm open- topped cells and flow cells can be used in a thermostattable cell holder. Tubing connector Tubing restraint tab Figure 13 Thermostattable cell holder Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 53: Installing A Thermostattable Cell Holder

    When the thermostattable cell holder is not in use, it is best to disconnect the tubing and drain the manifold. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 54: Cell Stirring Module

    When you put a magnetic stirring bar (9301- 1161) into the filled sample cell, the stirring bar rotates in tandem with the impeller magnet, thus stirring the sample. Figure 14 Cell stirring module Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 55: Installing The Cell Stirring Module

    4 Tighten the four screws (flathead, M3×6 mm) that hold the cell stirring module in place. 5 Attach the thermostattable cell holder to its base. 6 Mount the thermostattable cell holder back in the sample compartment. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 56: Tubing Connections And Operation

    • The stirring module can be used alone, without the thermostattable functions of the cell holder. • The same water supply can be used to regulate sample cell temperature and to power the cell stirring module. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 57 C (in), D (out) Without cell thermostatting A (in), B to D, C (out) In series with cell thermostatting A and C (in), B and D (out) In parallel with cell thermostatting Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 58: Long Path Cell Holder

    “Optical Filter Wheel” on page 61 for details. However, instead of a screwdriver, a 2.5- mm hex wrench is required to install the optical filter wheel on the long path cell holder. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 59: Installing The Long Path Cell Holder

    This example shows a 1- cm sample cell: Cell stops Cell clamps Position cell here Clamp screws Figure 18 Positioning the cell stops for a 1-cm sample cell Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 60 5 Allow the clamps to close against the sample cell, see Figure Tighten the four screws that hold the sample cell in place. Figure 19 Installed 10-cm cylindrical cell in the long path cell holder Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 61: Optical Filter Wheel

    100% transmittance at all light wavelengths. Positions one, two and three have filters which absorb varying amounts of the UV spectrum. Figure 21 shows the transmission characteristics of each filter. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 62 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 63: Installing The Filter Wheel Assembly

    4 Tilt the outer edge of the clamp up slightly. 5 Bring the filter wheel assembly against the cell holder so that the clamp fits in the slot of the filter wheel assembly. The mounting ridges on the Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 64: Removing The Filter Wheel Assembly

    2 Separate the filter wheel assembly from the cell holder. 3 The filter wheel clamp may be left in place or removed. In either case, retighten the two screws. 4 Store the filter wheel in a clean plastic bag. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 65: Installing The Uv-Visible Software

    Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide Installing the UV-Visible Software Agilent ChemStation Computer Details Installing the Agilent ChemStation Using the DVD This chapter describes the minimum hardware and software requirements and describes the installation process for the UV- Visible software.

  • Page 66: Agilent Chemstation Computer Details

    The recommended graphics resolutions for video graphics adapter are: 1024 x 768 with large fonts or 1280 x 1024 with large fonts Processor 3 GHz Screen resolution Super VGA (1280 × 1024) Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 67 Separator to "." (decimal point) in Control Panel > Region and Settings. Printer Please make sure that a default printer is configured in your Microsoft Windows operating system. This is done via the Control Panel application. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 68 If no default printer is configured, you may encounter the following problems: • the 'Copy To Clipboard' menu may have an error • in the 'Custom Report View' there may be problems with new templates • the 'Print Preview' will not work Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 69: Installing The Agilent Chemstation Using The Dvd

    Installing the Agilent ChemStation Using the DVD Installing the Agilent ChemStation Using the DVD Overview This section explains how to use the Agilent Technologies UV- Visible ChemStation Software Products DVD to: • install the Agilent ChemStation for the first time •...

  • Page 70: Installation Procedure

    1 Insert the Agilent UV- Visible ChemStation Products DVD into the DVD drive. 2 From the Start menu in the taskbar, select Start > Run. 3 At the command line, type diskdrive:\Setup.exe (for example, D:\ Setup.exe), then click OK. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 71 Using Windows 7 you may get the warning dialog below several times during the N O T E installation. Click Run to continue. 4 Read the licence agreement and click Yes to accept. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 72 One or the other can be installed, but not both. 7 Enter the License Registration Number for each selected module in the entry line and click Add License. When all required licenses are added, Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 73 The applicable product and license numbers are printed on your Software Certificate and Registration Packet (see the example license registration label). SOFTWARE REGISTRATION LABEL FOR PRODUCT NUMBER G1115AA REVISION CODE A.08.01 REGISTRATION NUMBER DU08E7777A Figure 23 Example license registration label Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 74: Monitor Configuration

    1024×768 or higher resolution, you may select a larger font. The font size is available with the control panels’s Display settings. In the Display properties dialog box use the Appearance tab for the adjustment. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 75: Configuring Your System

    The available GPIB addresses are displayed in the box. The spectrophotometer and accessories are shipped with the following default GPIB addresses: GPIB Address Instrument/Accessory Name Spectrophotometer, or Temperature control accessory Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 76 Choose Add New Instrument from the Add/Delete menu to add a new instrument window to the Configuration Editor screen. Follow the above procedure to configure the instrument. Choose Delete Instrument from the Add/Delete menu to remove an instrument from the configuration. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 77 Help in the UV- Visible ChemStation software. Autosamplers If you have an Agilent XY autosampler, your Agilent ChemStation software requires a special driver and a cable. See the readme.txt for further information. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 78 Installing the UV-Visible Software Installing the Agilent ChemStation Using the DVD Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 79: Gpib Communication, Connections And Configuration

    Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide GPIB Communication, Connections and Configuration GPIB Communication Installing an GPIB Interface Installation of the Agilent GPIB Communication Library Configuring the Agilent 82350 GPIB Interface Configuring the Agilent 82357 USB - GPIB Interface...

  • Page 80: Gpib Communication

    HP- IB (Hewlett- Packard Instrument Bus) and GPIB (General Purpose Interface Bus). GPIB communication can be used to control Analytical instruments by means of a controller. The Agilent Cary 8454 UV- Visible spectrophotometer and the optional Peltier Temperature Station 89090A have build- in GPIB interfaces.

  • Page 81: Operating System-Instrument Compatibility

    2 Select the appropriate empty slot for your GPIB card. The 82350A/B plugs into any PCI slot. It is best not to place the GPIB board next to a graphics interface. As Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 82: Gpib Cabling

    7 Connect GPIB devices in a chain. A chain occurs when an GPIB device is connected to the next GPIB device, and it is, in turn, connected to the next, and so on. Avoid star configurations (connecting all of the devices to a central point). Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 83 GPIB controller can induce electrical spikes that may potentially corrupt the GPIB protocol. In extreme cases. this may require cycling power on all instruments, including the GPIB controller (that is, typically the Agilent ChemStation). Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 84: Installation Of The Agilent Gpib Communication Library

    Program Group called “Agilent IO Libraries Suite” Windows 7 may request your permission to continue. N O T E Press the Run button to continue. Figure 24 Windows security warning Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 85 Figure 25 Agilent IO Libraries Suite installation Welcome screen 3 Click Next to continue and select I accept the terms of the license agreement. Figure 26 Agilent IO Libraries Suite license agreement Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 86 4 Click Next to continue and select Typical for the Setup Type. Figure 27 Agilent IO Libraries Suite setup type selection 5 Click Next to continue and press the Install button on the summary screen. Figure 28 Agilent IO Libraries Suite installation summary Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 87 Agilent IO Libraries Suite completion dialog As a result of your installation you should see the IO library icon on the Windows task bar. Figure 31 Agilent IO Libraries Suite quick launch icon Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 88: Configuring The Agilent 82350 Gpib Interface

    Local administrator rights are required to configure your interface. N O T E 1 Boot your PC, a tool tip will notify you about new hardware and the Found New Hardware Wizard will start. Select Option 2 and click Next Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 89 GPIB Communication, Connections and Configuration Configuring the Agilent 82350 GPIB Interface (see Figure 32) Figure 32 Found New Hardware Wizard 2 Select Option 2 and click Next (see Figure 33). Figure 33 Install hardware device drivers Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 90 GPIB Communication, Connections and Configuration Configuring the Agilent 82350 GPIB Interface 3 Select 82350 PCI and click Next (see Figure 34). The installation of the device driver will start. Figure 34 Select a device driver Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 91 Agilent Connection Expert. 6 Uncheck Show this window at startup and press Close Welcome Screen. The Agilent Connection Expert Main Window is displayed (see Figure 36). Figure 36 IO Libraries Configuration Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 92 Auto- discover instruments connected to this interface (see Figure 38). Figure 38 Agilent 82350 interface configuration. The appearance of the dialog slightly differs depending on the type of GPIB board installed. N O T E Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 93 Instrument I/O on this PC with a yellow warning symbol with an exclamation mark. Use the File menu’s Exit command and in the upcoming dialog click Reboot Now. After the reboot your GPIB library installation is complete. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 94: Configuring The Agilent 82357 Usb - Gpib Interface

    If a GPIB cable is used to interconnect multiple modules (e.g. 89090A Peltier Temperature N O T E controller) the USB Interface device must also be plugged into the GPIB connector (see Figure 39). Figure 39 82357 USB-GPIB interface on a spectrophotometer Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 95 Found New Hardware Wizard will start (see Figure 40). 3 Select Option 1 and click Next and the installation will start (see Figure 40). Figure 40 Found New Hardware Wizard, first detection Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 96 Figure 41 Found New Hardware Wizard, first detection 5 The system will initialize the USB device. This might take some seconds (see Figure 42). Figure 42 Initializing the 82357A USB-GPIB interface, first detection Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 97 7 The hardware wizard now detects the USB- GPIB device for a second time and the Found New Hardware Wizard starts again (see Figure 44). 8 Select Option 1 and click Next (see Figure 44). Figure 44 Found New Hardware Wizard, second detection Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 98 10 The system initializes the 82357A USB- GPIB Interface device. This might take some seconds (see Figure 46)). Wait until the configuration screen appears, see step 14. Figure 46 Installing the 82357A USB-GPIB interface, second detection Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 99 Configuring the Agilent 82357 USB - GPIB Interface The hardware installation warning may appear. If so, click Continue Anyway. N O T E Hardware Installation warning. 11 Click Finish to complete the hardware setup. Figure 47 HardwareWizard completion. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 100 13 Uncheck Show this window at startup and click Close Welcome Screen. The Agilent Connection Expert Main Window is displayed (see Figure 48). Figure 48 Agilent Connection Expert main window 82357 USB-GPIB interface Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 101 GPIB address: to 30, SICL Interface ID: to hp82341 (note that this is case sensitive), uncheck Auto- discover instruments connected to this interface (see Figure 50) Figure 50 Agilent 82357 interface configuration Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 102 16 Click OK to close the interface configuration dialog and use the File menu’s Exit command to quit the Agilent Connection Expert. Your GPIB library installation and 82357 interface configuration is complete. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 103: Upgrading Existing Io Libraries

    GPIB Communication, Connections and Configuration Upgrading Existing IO Libraries Upgrading Existing IO Libraries Upgrading IO Libraries Follow the installation procedure described “Installing New IO Libraries” on page 84. The existing library will be automatically removed. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 104: Working With Your Agilent Io Libraries

    To access the documentation, click on the IO Icon in the notification area of the Taskbar, choose Documentation and select the type of information you need (see Figure 51). Figure 51 Agilent IO Libraries Suite documentation access. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 105: Troubleshooting

    2 In the Add or Remove Programs dialog select Agilent IO Libraries Suite and click Change/Remove. After a few seconds of preparation the Agilent IO Libraries Suite - InstallShield Wizard is displayed (see Figure 52). Figure 52 Agilent IO Libraries Suite InstallShield Wizard Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 106 3 Select the Repair option and click Next. Wait until the repair process is finished. 4 Click Finish to complete the repair (see Figure 53). Figure 53 Agilent IO Libraries Suite maintenance complete. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 107: Problems With Plug And Play Option Gpib Cards

    PCI card is found. Select the second choice to search from a list of known devices and there select the option for Agilent Technologies, since the IO Libraries are already installed. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 108 7 To configure the GPIB card please follow the instructions in “Configuring the Agilent 82350 GPIB Interface” on page 88 or “Configuring the Agilent 82357 USB - GPIB Interface” on page 94. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 109: Changing The Gpib Address Of The Spectrophotometer

    1 and 2 are in the down position for GPIB address definition and set the switches 3 through 8 for the appropriate address, see Table 2 3 4 Figure 55 Configuration switch module Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 110 C A U T I O N to the same GPIB controller and the GPIB address of the controller itself. In our configuration the GPIB address 30 is used for the controller. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 111: Gpib Address Settings

    WA R N I N G switches. Any changes could result in instrument failure. To recover, set switches 1 and 2 to position 0 and power-cycle the instrument. For more detailed information, see the Service Manual. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 112 GPIB Communication, Connections and Configuration Changing the GPIB Address of the Spectrophotometer Table 6 GPIB address switch settings (addresses 16 through 31) Address Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 113: Lan Communication, Installation, Connection And Configuration

    Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide LAN Communication, Installation, Connection and Configuration Installing and Configuring TCP/IP on the PC Installing the LAN Interface in your UV-Visible Spectrophotometer Connecting the Spectrophotometer to the PC Using the Agilent BootP Service...

  • Page 114: Installing And Configuring Tcp/Ip On The Pc

    1 Select Start > Control Panel> Network and Sharing Center. In the Tasks list click on Manage network connections. In the LAN or High- Speed Internet list right click on your LAN adapter icon and select Properties. Figure 56 Local Area Connection Properties Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 115 ‘Subnet Mask’ if available. Ask your responsible IT consultant for this mask if not available and request a fixed IP address for your instrument, if you want to use the existing LAN for the instrument connection. For further details please see“Connecting the Spectrophotometer to the PC”. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 116 Gateway, DNS and WINS IP addresses do not need to be configured if you do not connect N O T E to any other part of a network. 6 Turn off the Microsoft Windows Firewall and reboot the computer. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 117: Installing The Lan Interface In Your Uv-Visible Spectrophotometer

    192.168.254.11 for the spectrophotometer. (note: to use these defaults, DIP switches 5 and 6 on the LAN card must be "ON"). 4 Plug the LAN interface card into the slot and screw down the two mounting screws. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 118: Connecting The Spectrophotometer To The Pc

    LAN interface cards to connect the switch to the spectrophotometer. Do not use the black 10 ft. long crossover twisted pair cable (part number 5183-4649) in an N O T E installation with multiple instruments. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 119: Setting The Ip Address Of The Spectrophotometer

    Agilent BootP Service” on page 120. If the system is on an isolated LAN and the default IP address 192.168.254.11 for the N O T E spectrophotometer is used, BootP is not necessary. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 120: Using The Agilent Bootp Service

    “Configuring the G1369C LAN Card Using BootP” on page 127. If you do not have a BootP Service, we suggest to use the Agilent BootP Service program supplied on the Agilent UV- Visible ChemStation DVD. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 121: Addresses

    Before installing and configuring BootP Service, you must know the IP addresses of the computer and instruments, the subnet mask, and the gateway. Table 7 Example Device Address 10.10.10.1 Spectrophotometer 10.10.10.101 Subnet mask 255.255.255.0 Gateway 10.10.10.1 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 122: Using The Agilent Bootp Service Program

    MAC address and IP address pair to the configuration. The Launch Manager is displaying a list of the currently configured MAC address and allows modification of the current configured settings. Figure 59 Configured interfaces view of the Agilent BootP Service Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 123: Configure The Agilent Bootp Service Program

    To remove the card from the system, turn the module off before removing the LAN card. Read the MAC address from the label. Reinstall the card and turn on the module again. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 124 Gateway Address 6 Click OK. 7 Exit Launch Manager and power cycle both the PC and the spectrophotometer. 8 Ping the IP address from a command prompt on the PC to verify. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 125: Configure The Agilent Bootp Service

    System and Security and select Administrative Tools > Services. The Services window appears. Figure 61 Services window 2 Right click Agilent BootP Service. 3 Select Stop. 4 Close the Services and Administrative Tools windows. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 126 System and Security and select Administrative Tools > Services. The Service screen appears. 12 Right click Agilent BootP Service. 13 Select Start. 14 Close the Services and Administrative Tools screen. 15 This completes the configuration. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 127: Configuring The G1369C Lan Card Using Bootp

    - ask your system administrator> #8454 lab UV-Vis:\ ht=1:\ ha=0030D30D3277:\ ip=<ip address - ask your system administrator> sm=255.255.255.0:\ gw=<gateway address - ask your system administrator> vm=auto:\ hn:\ bs=auto:\ T145=64:T146=01:T147=01 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 128 The entries are described below • #8454 lab Comment line starting the configuration set for an Agilent Cary 8454 UV- Visible spectrophotometer. A comment is preceded with #; all characters after the # on the same line are ignored by the BootP Service.

  • Page 129: Using The Agilent Chemstation Configuration Editor To Configure The Spectrophotometer

    LAN interface Card must be installed, the spectrophotometer must be connected to the PC, and a bootp server must be installed and configured before the spectrophotometer(s) can be used. See “Installing and Configuring TCP/IP on the PC” on page 114 for details. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 130: Configuring An Agilent Cary 8454 Uv-Visible Spectrophotometer

    Using the Agilent ChemStation Configuration Editor to Configure the Spectrophotometer Configuring an Agilent Cary 8454 UV-Visible Spectrophotometer This section describes how to configure an Agilent Cary 8454 UV- Visible spectrophotometer for LAN communication. 1 Start the Agilent ChemStation Configuration Editor by double- clicking on the Configuration Editor icon in the UV- Visible ChemStation start- up group, by choosing Start >...
  • Page 131 Add to add to the Selected Modules box. 6 Select Identify by IP Address and type in the IP address of the LAN interface card. (If a DNS server is installed you can also define the host name.) Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 132 Agilent ChemStation to check if the software communicates with the spectrophotometer. In case the red error message line Power Fail shows up, refer either to the following troubleshooting section or repeat the installation procedure. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 133: Troubleshooting Lan Communication

    If you identify the UV- Visible system by host name, ensure that the used host name and IP address are correctly setup in the DNS server. Try using the IP address in the Configuration Editor. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 134: Frequent Buffer Overrun In Agilent Cary 8454 Logbook

    Ask you IT consultant to make sure that no IP address conflict may occur. Frequent buffer overrun in Agilent Cary 8454 logbook Please contact your system administrator, to check if the network is not capable of the network traffic induced due to the data acquisition of the instrument(s).
  • Page 135 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide Safety Information Safety Information Warning and Caution Messages Lithium Batteries Information Agilent Technologies on the Internet Agilent Technologies...

  • Page 136: A Safety Information

    Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements. General...

  • Page 137: Safety Symbols

    Indicates a protected ground terminal. Eye damage may result from directly viewing light produced by deuterium lamps used in detectors and spectrophotometers. Always turn off the deuterium lamp before opening the lamp door on the instrument. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 138: Warning And Caution Messages

    WA R N I N G A triangular symbol indicates a warning. The meanings of the symbols that may appear with warnings in the documentation or on the instrument are: Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 139 The following symbol may be used on warning labels attached to the instrument. When you see this symbol, refer to the relevant operation or service manual for the correct procedure referred to by that warning label. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 140: Ce Compliance

    Declaration of Conformity. The Declaration of Conformity is the legal declaration by Agilent that the product complies with the directives listed above, and shows the EN standards to which the product was tested to demonstrate compliance. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 141: Electromagnetic Compatibility

    4 Make sure that all peripheral devices are also certified. 5 Make sure that appropriate cables are used to connect the device to peripheral equipment. 6 Consult your equipment dealer, Agilent Technologies, or an experienced technician for assistance. Changes or modifications not expressly approved by Agilent Technologies could void the user’s authority to operate the equipment.
  • Page 142 Safety Information Warning and Caution Messages ICES/NMB-001 This ISM device complies with Canadian ICES- 001. Cet appareil ISM est conforme à la norme NMB- 001 du Canada. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 143: Solvent Information

    • Analysis- grade ethers, which can contain peroxides (for example, THF, dioxane, di- isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 144 For example, a 1- % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra- acetic acid). • Mixtures of carbon tetrachloride with 2- propanol or THF. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 145: Lithium Batteries Information

    Transportation of discharged lithium batteries through carriers regulated by IATA/ICAO, ADR, RID, IMDG is not allowed. Discharged lithium batteries must be disposed of locally according to national waste disposal regulations for batteries. Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 146: Agilent Technologies On The Internet

    Safety Information Agilent Technologies on the Internet Agilent Technologies on the Internet For the latest information on products and services visit our website at: http://www.agilent.com Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...

  • Page 147: Index

    GPIB switch module, buffer handling, DIP switches, GPIO connector, dirty filters, ground connection, display, dust, cable GPIB, handling cells, carbon disulfide, hardware carbon tetrachloride, electronic self tests, installing, CE compliance, entrapment of bubbles, Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 148 40, photochemical reactions, signal to noise ratio, line frequency, photodegradation, single beam instrument, line power consumption, photographic lens tissues, sipper system, line power input socket, photosensitive substances, sipper/sampler system, Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 149 TalkToLab, temperature control, temperature fluctuations, temperatures, thermal equilibrium, thermostattable cell holder, thermostatted water, thermostatted water bath, toluene, trimethylpentane 2,2,4-trimethylpentane, turn on your spectrophotometer, turn-on self-test, useful wavelength range of solvents, Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 150 Index Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide...
  • Page 152 In This Book This handbook describes the steps needed to install your new Agilent Cary 8454 UV- Visible spectroscopy system. Most of the handbook deals with installing a complete system, that is, spectrophotometer, computer and printer. However, in the...

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