Agilent Technologies Cary 8454 Installation Manual page 44

Uv-visible spectroscopy system
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3
Good Measurement Practices
General Considerations
Figure 10
Lens tissues for glasses or other uses often contain detergents or
lubricants which can affect your measurements. If possible avoid cleaning
the faces of your cell between blank and sample measurements.
Handling Cells
Always install a cell so that it faces the same direction to minimize
problems with cell non- uniformity. For best results with microcuvettes,
leave your sample cell clamped in position throughout the measurement
sequence. Solutions should be removed and replaced by pipette or use
flow cells.
If glass pasteur pipettes are used, make sure that the optical windows of the cell are
C A U T I O N
not touched or scratched by the pipette.
44
0.01
0.00
-0.01
-0.02
-0.03
-0.04
-0.05
200
400
Spectrum taken with floating particles in the light path
Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
600
800
Wavelength [nm]
1000

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