Refractive Index Conversions - Beckman Coulter Optima XL Series Instruction Manual

Preparative ultracentrifuge
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REFRACTIVE INDEX CONVERSIONS

USER INPUTS
CALCULATIONS
To select this calculation (Figure 6-5), use the arrow keys to highlight it, then
✮✷✽✮✻❛
press
.
DENSITY/REFRACTIVE INDEX CONVERSIONS
CALCULATES VALUES OF DENSITY, REFRACTIVE INDEX, OR CONCENTRATIONS
GRADIENT SOLUTE =
ENTER ONE OF THE FOLLOWING:
R EFR AC TIVE INDEX
U S E K E Y P A D T O E N T E R O R M O D I F Y M O L A R I T Y .
A C C E P T A B L E R A N G E F R O M 1 . 3 3 0 T O 1 . 4 1 8 4
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FIELD
FIELD
Figure 6-5. Refractive Index Conversions
Enter the applicable input, depending on the calculation required.
REFRACTIVE INDEX—Use the keypad to enter a value, then press
✮✷✽✮✻❛
. (The default value is 1.3330, the RI of pure water at 20°C.)
DENSITY—Use the keypad to enter a density in g/mL within the range
permitted for the solute, then press
the density of pure water at 20°C.)
MOLARITY—Use the keypad to enter a value within the range permitted for
✮✷✽✮✻❛
the solute, then press
If a value for refractive index, density, or molarity is entered, values for the
other two parameters are calculated.
CsCl
@20 DEG C
=
g/ mL
DE NSITY
=
=
M OLAR IT Y
✮✷✽✮✻❛
. (The default value is 0.998 g/mL,
to calculate the other parameters.
Calculations
CALC
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6-7

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