Beckman Coulter Optima XL Series Instruction Manual page 87

NOTE
If you are performing consecutive zonal runs: at the end
of each zonal run, the centrifuge will automatically reset
to the normal mode. To reconfirm selection of the zonal
mode, you must turn the key left to right (clockwise)
from the zonal position to the normal position and then
back to the zonal position. Then press
✼✽✪✻✽❛
again to begin the run. If you press
✼✽✪✻✽❛
without having reset the key, the DOOR OPEN
diagnostic message will be displayed in the CURRENT
VALUES window, and the instrument will not start.
Press to clear the diagnostic; to begin operation,
ce
turn the key as described above and press
✼✽✪✻✽❛
again.
6. Load the rotor.
7. Disconnect the seal assembly and cap the rotor.
8. Close the chamber door. The vacuum system will activate automatically.
The rotor will hold at 3000 rpm (while displaying the message WAIT FOR
VAC) until the chamber pressure drops below 750 microns, at which time
it will accelerate to set speed and the instrument will begin counting time
remaining or time elapsed.
9. When the run is ended (time remaining at zero, ω
✼✽✸✹❛
pressed), the rotor decelerates to 2000 rpm.
NOTE
To abort a zonal run in progress, press
rotor speed decelerates to 2000 rpm, press
10. When the SPEED setting indicates that the rotor is spinning at 2000 rpm,
a series of beeps will sound; press
open the chamber door.
11. Unload the rotor according to the instructions in the zonal rotor manual.
✼✽✸✹❛
Press after unloading to bring the rotor to rest. Keep the door
closed between runs to help keep the chamber clean and dry.
NOTE
When the rotor is at rest, the instrument will automati-
cally reset to the normal mode and the ZONAL message
will disappear.
Summary of Run Procedures
✮✷✽✮✻❛
and
✮✷✽✮✻❛
✮✷✽✮✻❛
2
t value reached, or
✼✽✸✹❛
. When the
✼✽✸✹❛
✿✪✬✾✾✶❛
to vent the chamber. Then
and
and
again.
8-5