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6.2.2.4
Installation of accessories
Version 08/2012, Rev. 1.24 of 28/04/2022 • sb
Translation of the original operating manual, part no. 0700702
Operation
•
Fill the capillary tubes with blood and seal them at one end with putty or
by fusion.
•
Place the capillary tubes into the recesses of the rotor with the sealed
end against the rubber ring. Ensure that the capillary tubes fit tightly
against the rubber ring. The opposite places must be loaded.
•
Screw on the rotor cover.
•
Close the centrifuge lid.
•
Enter the following parameters: RCF 12,000 x g, runtime approx.
5 minutes.
•
Start the centrifuge.
•
Open the centrifuge lid when the rotor has stopped.
•
Unlock the rotor cover and lift it off.
Evaluation
•
Put the reader onto the microhaematocrit rotor.
•
Turn the reader and perform a fine adjustment with the aid of the central
eccentric mechanism in order to localize the O-point and the maximum
liquid point in the capillary tubes. You can now read the percentage
value.
•
Remove the capillary tubes. Some of the tubes can also be evaluated
with the reader outside of the rotor (see the instructions for use on the
back of the reader).
•
Only use vessels that are suitable for the rotor.
•
In the case of swing-out rotors, all of the compartments must be
equipped with buckets.
•
Always load opposite compartments of the rotors with the same
accessories and fill to avoid imbalance.
Centrifugation with vessels of various sizes
Working with vessel of various sizes is possible. In this case, however, it is
very important that the rotationally symmetrical inserts are identical.
correct
Fig. 6: Permissible and impermissible loading of a swing-out rotor with vessels of various
sizes (example illustration)
Refrigerated Centrifuge Sigma 2-16KL
6 Using the centrifuge
incorrect
33 / 82
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