Sigma 2-16K Operating Manual
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Sigma 2-16K Operating Manual

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Refrigerated Centrifuge
2-16K
Operating Manual

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  • Page 1 ——————————————— Refrigerated Centrifuge 2-16K Operating Manual...
  • Page 3 We are wishing you a successful use of the centrifuge. SIGMA Laborzentrifugen GmbH P.O. Box 1713 - 37507 Osterode/Germany Phone 05522/5007-0 - Fax 05522/500712 Internet: www.sigma-zentrifugen.de eMail: info@sigma-zentrifugen.de SIGMA Service-Tel. 05522/5007-25 Operating Manual SIGMA 2-16K, page 3 of 61 03/06...
  • Page 5 EN 61326 Sigma Laborzentrifugen An der Unteren Söse 50 D-37520 Osterode 01.08.2002 Geschäftsführer Managing Director Directeur Gérant ................ Fabr. Nr. Serial No. Numéro de fabrique Konformitätserklärung dreisprachig 2-16k und 2-16KC 20020801.DOC Operating Manual SIGMA 2-16K, page 5 of 61 03/06...

  • Page 7: General Information

    3.4.3 Installation of a rotor Operating Elements Operating panel 4.1.1 Start-key / Saving a program 4.1.2 Stop-key with preselection of the soft stop and/or soft start function 4.1.3 Lid-key 4.1.4 Knobs Operating Manual SIGMA 2-16K, page 7 of 61 03/06...

  • Page 8: Care And Maintenance

    Centrifuge decelerates during operation 7.2.3 Lid cannot be opened/closed 7.2.4 Emergency lid release 7.2.5 Problems with the centrifuge? Error mode 7.3.1 Error codes Diagram speed/gravitational field Declaration of decontamination/Return declaration Leaflet Operating Manual SIGMA 2-16K, page 8 of 61 03/06...

  • Page 9: Technical Data

    %. The minimum temperatures during run are < +4 ° C a nd depend on type of rotor, speed and ambient temperature. For rotor 12132 approx. +6° C at 14000 rpm. (Allowable ambient temperature +10 ° C - +35 ° C; max . humidity 80 %.) Subject to technical alterations. Operating Manual SIGMA 2-16K, page 9 of 61 03/06...
  • Page 11 12148 Angle rotor 24 x 1.5-2.2 ml for reaction vials e.g. 15008, 15040, incl. hermetic aluminium lid, max. radius 8.2 cm, min. radius 5 cm, angle 45° 15 300 21 460 Operating Manual SIGMA 2-16K, page 11 of 61 03/06...
  • Page 12 9 000 8 603 13060 Adapter for 1 culture tube 15 ml 15115, suitable for 12151, 13150, 19776 13079 Bottom adapter for 1 tube 40-42 ml 15051, 15052, 15054, suitable for 12151 Operating Manual SIGMA 2-16K, page 12 of 61 03/06...
  • Page 13 ∅ ∅ ∅ ∅ 24.5 x 95 - 110 mm, e.g. 15025, 15026, 15033 13047 Bucket, aluminium, incl. rubber cushion 16051, for 1 tube 50 ml, max. ∅ ∅ ∅ ∅ 35.5 x 95 - 110 mm, e.g. 15049, 15050, 15056 Operating Manual SIGMA 2-16K, page 13 of 61 03/06...
  • Page 14 Round carrier for 1 tube 50 ml, max. ∅ ∅ ∅ ∅ 14032 35/38 x 70 - 105 mm, e.g. 15049, 15050, 15056, polypropylene, suitable for 13041 17130 Sealing cap, polysulfone, clear, for 13041 Operating Manual SIGMA 2-16K, page 14 of 61 03/06...
  • Page 15 , no. 03008 www.bibby-sterilin.co.uk Round carrier for 1 tube 50 ml, max. ∅ ∅ ∅ ∅ 17250 35/38 x 70 - 105 mm, e.g. 15049, 15050, 15056, polyethylene, suitable for 13040 Operating Manual SIGMA 2-16K, page 15 of 61 03/06...
  • Page 16 1 pack contains 100 pcs., suitable for 13002 15008 Reaction vials 1.5 ml, 1 pack contains 100 pcs., suitable for 11124, 12132, 12148 15040 Reaction vials 2.2 ml, 1 pack contains 100 pcs., suitable for 11124, 12132, 12148 Operating Manual SIGMA 2-16K, page 16 of 61 03/06...
  • Page 17 0 - 50 ml in steps of 1 ml, ∅ ∅ ∅ ∅ 34 x 100 mm, suitable for 13047 Teflon tube with screw cap 42 ml, ∅ ∅ ∅ ∅ 28.5 x 15051 107 mm, suitable for 13079 Operating Manual SIGMA 2-16K, page 17 of 61 03/06...
  • Page 18 Centrifuge glass tube 100 ml, ∅ ∅ ∅ ∅ 44 x 15100 100 mm, suitable for 11190, 13097 15106 ditto, graduated, 1 - 100 ml in steps of 1 ml Further accessories available on request. Operating Manual SIGMA 2-16K, page 18 of 61 03/06...

  • Page 19: Maximum Speed For Tubes

    We recommend to always fill up the tubes and to follow the recommendations of the manufacturer. Operating Manual SIGMA 2-16K, page 19 of 61 03/06...
  • Page 20 Operating Manual SIGMA 2-16K, page 20 of 61 03/06...

  • Page 21: Scope Of Supply

    Rotor Part No. Rotor No..............................................................Standards and Regulations Please refer to the enclosed EU-Statement of Conformity. Operating Manual SIGMA 2-16K, page 21 of 61 03/06...

  • Page 22: Safety Instructions

    Attention! Defective lid relieving devices could cause the centrifuge lid to fall down (contact Service). Risk of bruising! Operating Manual SIGMA 2-16K, page 22 of 61 03/06...

  • Page 23: Symbol Table

    Tirer la fiche de prise Vorsicht Quetschgefahr Caution! Risk of bruising Attention! Danger de blessure Drehrichtungspfeil Arrow direction of rotation → Flèche sens de rotation Heiße Oberfläche Hot surface Surface chaude Operating Manual SIGMA 2-16K, page 23 of 61 03/06...

  • Page 24: Construction And Constructive Safety Measures

    General Outlay The new generation of SIGMA laboratory centrifuges is equipped with newest state-of-the-art electronics and is driven by brushless, silent and long-life asyn- chronous motors. The problem of carbon brush change is no longer existent and as there is no carbon dust pollution, operation in clean rooms is possible if the appropriate accessories are used.
  • Page 25 “Imbalance“ is flashing and “ERROR“ is displayed. The centrifuge has to be opened with the lid key. In both cases the loading has to be checked and balanced. Operating Manual SIGMA 2-16K, page 25 of 61 03/06...

  • Page 26: Unpacking Of The Centrifuge

    Please keep case for possible transport of centrifuge later. 3.1.1 Transport Safety Device (made of foamed material) The SIGMA 2-16K has a transport safety device between the rotor chamber and the motor. It is accessible after opening of the centrifuge lid (refer to chapter 3.3 up to 3.4.2 or 7.2.4“...

  • Page 27: Installation

    The operating voltage on the name plate must correspond to the local supply voltage! SIGMA laboratory centrifuges are units of safety class I, DIN VDE 0700, and in- clude a three wire power cord 2,5 m long with shockproof right angle plug. The instrument has thermal fuses.

  • Page 28: Installation Of Rotors And Accessories

    Should the centrifuge be operated with very uneven load, the imbalance monitoring will switch off the drive. An imbalance warning would be displayed (refer to 4.1). Operating Manual SIGMA 2-16K, page 28 of 61 03/06...
  • Page 29 To increase life of rotors and seals the rotors must be cleaned with slushing oil and the seals and thread areas with vaseline or grease after cleaning. Attention! Please follow the special comments of chapter 1.5. Operating Manual SIGMA 2-16K, page 29 of 61 03/06...
  • Page 30 Operating Manual SIGMA 2-16K, page 30 of 61 03/06...

  • Page 31: Initial Start-Up

    Put a rotor onto the shaft and fasten it by screwing the rotor tie-down screw clockwise onto the drive shaft. Please use the supplied rotor wrench (refer to chapter 3.3 "Installation of rotors and accessories"). Operating Manual SIGMA 2-16K, page 31 of 61 03/06...

  • Page 32: Operating Panel

    The centrifuge is operated via the operating panel. Keys can be pressed when their LED is on. When power is applied, all LEDs and the display are illuminated for a short time. Operating Manual SIGMA 2-16K, page 32 of 61 03/06...
  • Page 33 Activation or deactivation of “soft stop“ and/or “soft start“ by pressing the stop-key. the centrifuge is running, stop-key illuminated: Press the stop-key. “soft stop“ and/or “soft start“ can be activated or deactivated during deceleration by pressing the stop-key again. Operating Manual SIGMA 2-16K, page 33 of 61 03/06...
  • Page 34 Remark: “set“ is automatically deactivated after 60 seconds, if no further changes are made, the lock symbol appears. Thereafter, changes are impossible. Right knob: Preselection of the desired set value after activation of “set“. Operating Manual SIGMA 2-16K, page 34 of 61 03/06...
  • Page 35 Symbol lock Temperature Speed Rotor, Imbalance, run mode 4.2.1 Speed/RCF (Relative Centrifugal Force) Rotor dimensions and speed determine the RCF value. Entry of one value automati- cally determines the other one. Operating Manual SIGMA 2-16K, page 35 of 61 03/06...

  • Page 36: Relative Centrifugal Force (Rcf)

    Preselection of 1 x g by pressing and holding (2 – 3 seconds) the stop key. Please refer to chapter 1.2 “Suitable accessories“ for the maximum values for the used rotor combination. Operating Manual SIGMA 2-16K, page 36 of 61 03/06...
  • Page 37 The time value as well as different time modes can be set by turning the right knob. If the time is changed during centrifugation, the total newly entered period is run, time elapsed before is not considered. Operating Manual SIGMA 2-16K, page 37 of 61 03/06...
  • Page 38 The set time is indicated by one single bar. The centrifuge run can be early terminated by pressing the stop-key. The set time and the remaining run time remain on the display. Operating Manual SIGMA 2-16K, page 38 of 61 03/06...

  • Page 39: Continuous Operation Mode

    The elapsed time is displayed in minutes. The continuous run mode can be left by selecting a set value as well. 4.2.3.2 Short-Time Operation There are two options: Operating Manual SIGMA 2-16K, page 39 of 61 03/06...
  • Page 40 A sequence of bars is indicating the elapsed run time. One single bar at "short run" indicates that short-time operation had been selected. After termination of the short-run the elapsed run time in seconds remains on the display. Operating Manual SIGMA 2-16K, page 40 of 61 03/06...
  • Page 41 The short-run can be early terminated by pressing the stop-key. The centrifuge decelerates to standstill. The elapsed run time in seconds remains on the display. The "short run“ can be left by selecting a set value as well. Operating Manual SIGMA 2-16K, page 41 of 61 03/06...
  • Page 42 This would reduce life of the compressor. We recommend precooling at 5000 rpm. The obtainable temperatures depend on rotor type, speed and ambient temperature. (A change of the limit values might cause deviations.) Operating Manual SIGMA 2-16K, page 42 of 61 03/06...
  • Page 43 Changed to rotor 12139 !!! Attention, special case: !!! If the rotors 12072, 12104 or 12105 are not preselected, rotor 12073 is automatically displayed for 12072 and rotor 12132 for 12104 and 12105. Operating Manual SIGMA 2-16K, page 43 of 61 03/06...
  • Page 44 The vessels should be placed symmetri- cally so that the rotor is loaded evenly. Loading e.g. only one position is not allowed. 9. Load all positions of swing-out rotors. 10. Load vessels outside the centrifuge. Operating Manual SIGMA 2-16K, page 44 of 61 03/06...
  • Page 45 17. When centrifuging substances with a density > 1,2 g/cm the allowable max. speed must be reduced (refer to chapter 7.1.2 "Density"). 18. Grease joints of buckets and rotor pins in swing-out rotors. Operating Manual SIGMA 2-16K, page 45 of 61 03/06...
  • Page 46 14. Lifting or moving of the centrifuge during operation. Leaning against or resting on the centrifuge is not allowed. 15. Do not place potential dangerous material - e.g. glass vessels containing liquids - near the centrifuge. Operating Manual SIGMA 2-16K, page 46 of 61 03/06...
  • Page 47 19. Substances which could damage the material of the centrifuge, the rotors or the adapters must not be centrifuged. Infectious, toxic, pathogene or radioactive substances must be centrifuged in certified rotors and vessels only and all necessary safety precautions are taken. Operating Manual SIGMA 2-16K, page 47 of 61 03/06...
  • Page 48 In order to avoid corrosion, rotor incl. tie-down screw and cover seal and adapters must be cleaned and greased regularly with the supplied slushing oil (Sigma part no.: 70104 for 20 ml slushing oil). Before using detergents or decontamination agents which had not been recommended by us, the user has to contact us to make sure that such procedure would not damage the centrifuge.

  • Page 49: Glass Breakage

    The condenser should therefore be regularly checked for dirt and cleaned if necessary. Generally blowing of the lamella with compressed air from inside and outside is sufficient (or a simple hoovering with a vacuum cleaner). Operating Manual SIGMA 2-16K, page 49 of 61 03/06...
  • Page 50 Principally we want to point out that for centrifuging of e.g. infectious material certified and hermetically sealed accessories have to be used in order to avoid that the centrifuge is contaminated. Operating Manual SIGMA 2-16K, page 50 of 61 03/06...
  • Page 51 Rubber cushions 115-118 Round carriers for 13104/ 115-118 13117, Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Round carriers for 13350/ 115-118 13550, Polypropylene Rectangular carriers, 115-118 Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Operating Manual SIGMA 2-16K, page 51 of 61 03/06...
  • Page 52 50.000 cycles. Any changes like e.g. corrosion, cracks, material abrasion etc. require special attention. 5. Screw connections have to be tight. Furthermore, the earth wire must be checked regularly. Operating Manual SIGMA 2-16K, page 52 of 61 03/06...
  • Page 53 If the density of the liquid is higher than 1.2 g/cm , the allowed maximum speed of the centrifuge is reduced according to the following formula: n = n , 1 ( Rho = density in g/cm Operating Manual SIGMA 2-16K, page 53 of 61 03/06...

  • Page 54: Error Correction

    (please refer to chapter 7.3.1 "Error codes"). 7.2.3 Lid cannot be Opened/Closed − When first trying to open the lid the locks are not released. The Lid-key LED is flashing. Open and close lid again. Operating Manual SIGMA 2-16K, page 54 of 61 03/06...
  • Page 55 Temperature sensor Excess temperature (>50° C) • Let centrifuge cool down Should it not be possible to repair the failure, please contact Service! Speed-RCF-Diagram An additional help is the enclosed Speed-RCF-Diagram. Operating Manual SIGMA 2-16K, page 55 of 61 03/06...

  • Page 56: Declaration Of Decontamination / Return Declaration

    Fill in the forms and attach them when returning centrifuges, accessories and spare parts. Please understand that we cannot carry out any work before we have the declarations. (We recommend to make several copies of this page.) Operating Manual SIGMA 2-16K, page 56 of 61 03/06...
  • Page 57 Unit / component contaminated : Unit / component unused (new) : !!! Attention – This form must be glued on outside of the box !!! ---------------------------------------------------------------------------------------------------------------------- Please make some copies before removing this page. Operating Manual SIGMA 2-16K, page 57 of 61 03/06...
  • Page 59 I / we hereby declare that the information on this declaration are correct and complete. Company / Institute : Street : Postcode, City : Tel. : FAX : Name : Date : Stamp : Signature : ---------------------------------------------------------------------------------------------------------------------- Please make some copies before removing this page. Operating Manual SIGMA 2-16K, page 59 of 61 03/06...
  • Page 61 Operating Manual SIGMA 2-16K, page 61 of 61 03/06...

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