Sigma 2-16KC Operating Manual
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Sigma 2-16KC Operating Manual

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Refrigerated Centrifuge
2-16KC
Operating Manual

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  • Page 1 — — — — — — — — — — — — — — — Refrigerated Centrifuge 2-16KC Operating Manual...
  • Page 3 We are wishing you a successful use of the centrifuge. SIGMA Laborzentrifugen GmbH P.O. Box 1713 - 37507 Osterode/Germany Phone 05522/5007-0 - Fax 05522/500712 Internet: www.sigma-zentrifugen.de eMail: info@sigma-zentrifugen.de SIGMA Service-Tel. 05522/5007-25 Operating Manual SIGMA 2-16KC, page 3 of 91 10/06...
  • Page 5 Sigma Laborzentrifugen An der Unteren Sö se 50 D-37520 Osterode 01.08.2002 Geschäftsführer Managing Director Directeur Gé rant ................ Fabr. Nr. Serial No. Numé ro de fabrique Konformitätserklärung dreisprachig 2-16k und 2-16KC 20020801.DOC Operating Manual SIGMA 2-16KC, page 5 of 91 10/06...

  • Page 7: General Information

    Fuses / emergency circuit breaker on site Installation of rotors and accessories 3.3.1 Fastening of angle rotors with hermetically sealed lid Initial start-up 3.4.1 Switching on of the centrifuge 3.4.2 Opening lid 3.4.3 Installation of a rotor Operating Manual SIGMA 2-16KC, page 7 of 91 10/06...

  • Page 8: Operating Elements

    Alteration of existing curves 5.1.5 Alteration of the contrast 5.1.6 Imbalance monitoring 5.1.7 Shortrun and faststop Programming Load, save and delete programs 6.1.1 Load a program 6.1.2 Save a program 6.1.3 Delete a program Operating Manual SIGMA 2-16KC, page 8 of 91 10/06...

  • Page 9: Care And Maintenance

    9.5.2 Centrifuge decelerates during operation 9.5.3 Lid cannot be opened/closed 9.5.4 Emergency lid release 9.5.5 Problems with the centrifuge? Error codes Speed-RCF-diagram Declaration of decontamination/Return declaration Form program data 9.10 Leaflet Operating Manual SIGMA 2-16KC, page 9 of 91 10/06...

  • Page 11: Technical Data

    10 °C - 35 °C; max. humidity 80 %.) Optional heating facilities: In order to obtain the final temperature the rotor incl. buckets has to be preheated at approx. 1500 rpm. Subject to technical alterations. Operating Manual SIGMA 2-16KC, page 11 of 91 10/06...
  • Page 13 12148 Angle rotor 24 x 1.5-2.2 ml for reaction vials e.g. 15008, 15040, incl. hermetic aluminium lid, max. radius 8.2 cm, min. radius 5 cm, angle 45° 15 300 21 460 Operating Manual SIGMA 2-16KC, page 13 of 91 10/06...
  • Page 14 9 000 8 603 13060 Adapter for 1 culture tube 15 ml 15115, suitable for 12151, 13150, 19776 13079 Bottom adapter for 1 tube 40-42 ml 15051, 15052, 15054, suitable for 12151 Operating Manual SIGMA 2-16KC, page 14 of 91 10/06...
  • Page 15 ∅ 24.5 x 95 - 110 mm, e.g. 15025, 15026, 15033 13047 Bucket, aluminium, incl. rubber cushion 16051, for 1 tube 50 ml, max. ∅ 35.5 x 95 - 110 mm, e.g. 15049, 15050, 15056 Operating Manual SIGMA 2-16KC, page 15 of 91 10/06...
  • Page 16 Round carrier for 1 tube 50 ml, max. ∅ 14032 35/38 x 70 - 105 mm, e.g. 15049, 15050, 15056, polypropylene, suitable for 13041 17130 Sealing cap, polysulfone, clear, for 13041 Operating Manual SIGMA 2-16KC, page 16 of 91 10/06...
  • Page 17 13040, , no. 03008 www.bibby-sterilin.co.uk Round carrier for 1 tube 50 ml, max. ∅ 17250 35/38 x 70 - 105 mm, e.g. 15049, 15050, 15056, polyethylene, suitable for 13040 Operating Manual SIGMA 2-16KC, page 17 of 91 10/06...
  • Page 18 1 pack contains 100 pcs., suitable for 13002 15008 Reaction vials 1.5 ml, 1 pack contains 100 pcs., suitable for 11124, 12132, 12148 15040 Reaction vials 2.2 ml, 1 pack contains 100 pcs., suitable for 11124, 12132, 12148 Operating Manual SIGMA 2-16KC, page 18 of 91 10/06...
  • Page 19 0 - 50 ml in steps of 1 ml, ∅ 34 x 100 mm, suitable for 13047 Teflon tube with screw cap 42 ml, ∅ 28.5 x 15051 107 mm, suitable for 13079 Operating Manual SIGMA 2-16KC, page 19 of 91 10/06...
  • Page 20 Centrifuge glass tube 100 ml, ∅ 44 x 15100 100 mm, suitable for 11190, 13097 15106 ditto, graduated, 1 - 100 ml in steps of 1 ml Further accessories available on request. Operating Manual SIGMA 2-16KC, page 20 of 91 10/06...

  • Page 21: Maximum Speed For Tubes

    We recommend to always fill up the tubes and to follow the recommendations of the manufacturer. Operating Manual SIGMA 2-16KC, page 21 of 91 10/06...
  • Page 22 Operating Manual SIGMA 2-16KC, page 22 of 91 10/06...

  • Page 23: Scope Of Supply

    Rotor Part No. Rotor No..............................................................Standards and Regulations Please refer to the enclosed EU-Statement of Conformity. Operating Manual SIGMA 2-16KC, page 23 of 91 10/06...

  • Page 24: Safety Instructions

    Attention! Defective lid relieving devices could cause the centrifuge lid to fall down (contact Service). Risk of bruising! Operating Manual SIGMA 2-16KC, page 24 of 91 10/06...

  • Page 25: Symbol Table

    Tirer la fiche de prise Vorsicht Quetschgefahr Caution! Risk of bruising Attention! Danger de blessure Drehrichtungspfeil → Arrow direction of rotation Flèche sens de rotation Heiße Oberfläche Hot surface Surface chaude Operating Manual SIGMA 2-16KC, page 25 of 91 10/06...

  • Page 26: Construction And Constructive Safety Measures

    General Outlay The new generation of SIGMA laboratory centrifuges is equipped with two microprocessors which guarantee independently the control of the rotor recognition and the overspeed signal. A further optimization and increase of the instrument’ s safety could be maintained. The long-life asynchronous motor is silent and brushless.

  • Page 27: Electronic Control

    (refer to chapter 9.5.4 "Emergency lid release"). 2.6.2 Imbalance Monitoring System In the event that uneven loading leads to imbalance, the drive is switched off and an imbalance warning message is displayed. Operating Manual SIGMA 2-16KC, page 27 of 91 10/06...
  • Page 28 Ground Wire Check For ground wire check there is a ground screw at the rear panel of the centrifuge. A ground wire check can be carried out using an appropriate measuring instrument. Operating Manual SIGMA 2-16KC, page 28 of 91 10/06...

  • Page 29: Unpacking Of The Centrifuge

    Please keep case for possible transport of centrifuge later. 3.1.1 Transport Safety Device (made of foamed material) The SIGMA 2-16KC has a transport safety device between the rotor chamber and the motor. It is accessible after opening of the centrifuge lid (refer to chapter 3.3 up to 3.4.2 or 9.5.4“...

  • Page 30: Installation

    The operating voltage on the name plate must correspond to the local supply voltage! SIGMA laboratory centrifuges are units of safety class I, DIN VDE 0700, and in- clude a three wire power cord 2,5 m long with shockproof right angle plug. The instrument has thermal fuses.

  • Page 31: Installation Of Rotors And Accessories

    12. Attention: The centrifuge will absorb smaller differences in weight when loading the rotors. But it is recommended to balance the vessels as accurately as possible in order to ensure a run with minimal vibrations. Operating Manual SIGMA 2-16KC, page 31 of 91 10/06...
  • Page 32 To increase life of rotors and seals the rotors must be cleaned with slushing oil and the seals and thread areas with vaseline or grease after cleaning. Attention! Please follow the special comments of chapter 1.5. Operating Manual SIGMA 2-16KC, page 32 of 91 10/06...
  • Page 33 Operating Manual SIGMA 2-16KC, page 33 of 91 10/06...

  • Page 34: Initial Start-Up

    3.3 "Installation of rotor and accessories"). Please pay attention to the fact that during tightening the disc spring of the rotor tie-down screw is pressed together and the screw is tightened. Operating Manual SIGMA 2-16KC, page 34 of 91 10/06...

  • Page 35: Operating Panel

    Operating Panel Operating panel The centrifuge can be operated via the operating panel. Keys can be pressed when their LED is on. Operating Manual SIGMA 2-16KC, page 35 of 91 10/06...
  • Page 36 This key is used to open the lid. This can only be executed if • the centrifuge has come to a complete stop • the Lid-key is illuminated. 4.1.4 Knob This knob can be used for selecting and alterating parameters and figures/ numbers. Operating Manual SIGMA 2-16KC, page 36 of 91 10/06...

  • Page 37: Relative Centrifugal Force (Rcf)

    The set value of this parameter is in the upper section of this area, underneath is the actual value. The maximal RCF-values are rotor dependent. (Refer to chapter 9.4 "Mathematical relations") Operating Manual SIGMA 2-16KC, page 37 of 91 10/06...
  • Page 38 By entering a specific run time the continuous mode is deactivated. It can be terminated by pressing the Stop-key as well. Continuous run Operating Manual SIGMA 2-16KC, page 38 of 91 10/06...

  • Page 39: Temperature (Temp)

    1 000 rpm. With an ambient temperature of +23 °C the obtainable lowest temperature is < +4 °C at maximum speed. The actual run temperatures depend on rotor type, speed and ambient temperature. Operating Manual SIGMA 2-16KC, page 39 of 91 10/06...

  • Page 40: Program (Prog)

    Storage space for fifty programs - No. 1 - 50- from which you can select and load a program is at your disposal. The actual loaded program is indicated by "--". Operating Manual SIGMA 2-16KC, page 40 of 91 10/06...
  • Page 41 In this area the actually selected rotor or a rotor/bucket combination are displayed. Rotor Selection List Rotor selection list The area marked with arrows for the rotor selection list offers all available rotors which can be selected via the knob. Operating Manual SIGMA 2-16KC, page 41 of 91 10/06...
  • Page 42 0 - 9 linear 10 - 19 quadratic up to 1000 rpm, then linear Their shape is further explained in chapter 9.1 "Slope of the specified curves, linear curves" and 9.2 "Quadratic curves". Operating Manual SIGMA 2-16KC, page 42 of 91 10/06...
  • Page 43 If the density of a sample exceeds 1.2 g/cm , the maximum final speed will reduce (refer to chapter 9.4.2 “ Density” ). A value between 1.2 and 9.9 g/cm possible. Operating Manual SIGMA 2-16KC, page 43 of 91 10/06...

  • Page 44: Start Delay

    With this function a start delay can be set. 4.2.8.7 Automatic Lid Opening after End of Run If this parameter has been set the lid will automatically open after standstill of the rotor. Operating Manual SIGMA 2-16KC, page 44 of 91 10/06...
  • Page 45 Selection of configuration (CONFIG) opens a menu where Code, Language, Screen, Fine, Buzzer, Sensor, Info, Reset and the creation of freely programmable curves can be chosen. Furthermore, the cycles and run times of the individual rotors can be read. Operating Manual SIGMA 2-16KC, page 45 of 91 10/06...
  • Page 46 Then, the centrifuge starts with the selected values. Notice: All entry limits are automatically checked. When reaching a limit, the counting operation is stopped (refer to chapter 9.3 "Entry limitations"). Operating Manual SIGMA 2-16KC, page 46 of 91 10/06...
  • Page 47 • Confirm the selected parameter by pressing the knob (here: acceleration). The parameter is activated. Select the desired acceleration curve by turning the knob. Deceleration, radius and density are changed in the same way. Operating Manual SIGMA 2-16KC, page 47 of 91 10/06...
  • Page 48 Select time delay (here: 10 seconds) by turning and confirm by pressing the knob. • Change the set time by turning the knob and deactivate the start delay by pressing the knob. Operating Manual SIGMA 2-16KC, page 48 of 91 10/06...
  • Page 49 Attention: Please take care that the correct rotor/bucket combination is entered as otherwise the max. allowable speed could be exceeded. This is not allowed. Operating Manual SIGMA 2-16KC, page 49 of 91 10/06...
  • Page 50 5.1 "Selection, Display and Alteration of Program Parameters"). You cannot change: • Rotor • Program • Radius • Density The following functions can be activated/deactivated: • Precooling • Start delay • Automatic lid opening after end of run Operating Manual SIGMA 2-16KC, page 50 of 91 10/06...
  • Page 51 The sensor menu displays different signals. In case of a failure of the instrument this makes the diagnosis of errors and their fast repair through the service easier. Values can neither be entered nor altered. Operating Manual SIGMA 2-16KC, page 51 of 91 10/06...
  • Page 52 - RCF magnified -Zoom- (no speed display) • Select the configuration mode by turning the knob. • After pressing the knob the configuration menu appears. • Select "SCREEN" by turning the knob. Operating Manual SIGMA 2-16KC, page 52 of 91 10/06...
  • Page 53 • Select "EXIT" by turning the knob. Confirm it by pressing the knob. You get into the configuration menu again. • By pressing the knob you return to the main display. The areas "SPEED" and "TIME" are displayed magnified. The area "RCF" disappears. Operating Manual SIGMA 2-16KC, page 53 of 91 10/06...
  • Page 54 The max. preset final speed of one interval corresponds to the set speed of the run. The total time corresponds to the set time of the run. The final speed of the last interval is 0. Operating Manual SIGMA 2-16KC, page 54 of 91 10/06...
  • Page 55 • Select the configuration mode by turning the knob. • After pressing the knob the configuration menu appears. • Select "CURVES" by turning the knob. • After pressing the knob the curve menue appears. Operating Manual SIGMA 2-16KC, page 55 of 91 10/06...
  • Page 56 Activate “ 0:00:00” by pressing the knob. • Set the time by turning the knob. • Save the time value in interval no. 1 by pressing the knob. • Select “ SPEED” by turning the knob. Operating Manual SIGMA 2-16KC, page 56 of 91 10/06...
  • Page 57 • After pressing and turning the knob the time value of interval no. 2 appears. • Activate “ 0:00:00” of the second interval by pressing the knob. • Set the time by turning the knob. The extended curve appears. Operating Manual SIGMA 2-16KC, page 57 of 91 10/06...
  • Page 58 • Activate the speed value “ 0” by pressing the knob. • Change the speed value by turning the knob. • After pressing and turning the knob the time value of interval no. 3 appears. Operating Manual SIGMA 2-16KC, page 58 of 91 10/06...
  • Page 59 • Activate “ 0” by pressing the knob. • Change the speed value by turning the knob. The curve appears and can be programmed as acceleration or deceleration curve under no. 20. Operating Manual SIGMA 2-16KC, page 59 of 91 10/06...
  • Page 60 If you press the knob for longer than a second, a dialogue window appears. Now you can select the contrast by turning the knob. By pressing the knob again, you confirm the entry, the new contrast remains. Operating Manual SIGMA 2-16KC, page 60 of 91 10/06...
  • Page 61 • Faststop Pressing the Stop-key during operation for longer than a second leads to maximum deceleration until standstill. Operating Manual SIGMA 2-16KC, page 61 of 91 10/06...
  • Page 62 Programs can be loaded, operated, altered, and deleted any time. All programs can be protected against unauthorized use by a personal Code. Operating Manual SIGMA 2-16KC, page 62 of 91 10/06...
  • Page 63 • Select the program selection list ( ) by turning the knob and activate this area by pressing the knob. You are able to see all programs in the memory, "--" indicates the actual program. Program selection list Operating Manual SIGMA 2-16KC, page 63 of 91 10/06...
  • Page 64 • Select the program you want to load by turning the knob. After pressing the knob a dialogue window appears. Select the instruction "LOAD" and confirm it by pressing the knob. Dialogue window Operating Manual SIGMA 2-16KC, page 64 of 91 10/06...
  • Page 65 • Select the desired program position in the list by turning the knob. • After pressing the knob a dialogue window appears. Select "SAVE" and confirm it by pressing the knob. Dialogue window Operating Manual SIGMA 2-16KC, page 65 of 91 10/06...

  • Page 66: Delete A Program

    • Select the program you want to delete by turning the knob. • A dialogue window appears after pressing the knob. Select "DELETE" and confirm it by pressing the knob. Dialogue window Operating Manual SIGMA 2-16KC, page 66 of 91 10/06...
  • Page 67 The vessels should be placed symmetri- cally so that the rotor is loaded evenly. Loading e.g. only one position is not allowed. 9. Load all positions of swing-out rotors. 10. Load vessels outside the centrifuge. Operating Manual SIGMA 2-16KC, page 67 of 91 10/06...
  • Page 68 2. The glass tubes tend to get stuck to the rubber cushions at higher tempera- tures. This can be avoided by applying e.g. talcum to the cushions prior to each run. Operating Manual SIGMA 2-16KC, page 68 of 91 10/06...
  • Page 69 14. Lifting or moving of the centrifuge during operation. Leaning against or resting on the centrifuge is not allowed. 15. Do not place potential dangerous material - e.g. glass vessels containing liquids - near the centrifuge. Operating Manual SIGMA 2-16KC, page 69 of 91 10/06...
  • Page 70 19. Substances which could damage the material of the centrifuge, the rotors or the adapters must not be centrifuged. Infectious, toxic, pathogene or radioactive substances must be centrifuged in certified rotors and vessels only and all necessary safety precautions are taken. Operating Manual SIGMA 2-16KC, page 70 of 91 10/06...
  • Page 71 In order to avoid corrosion, rotor incl. tie-down screw and cover seal and adapters must be cleaned and greased regularly with the supplied slushing oil (Sigma part no.: 70104 for 20 ml slushing oil). Before using detergents or decontamination agents which had not been recommended by us, the user has to contact us to make sure that such procedure would not damage the centrifuge.

  • Page 72: Glass Breakage

    Vaseline. Then the rotor should rotate for some minutes at a moderate speed. The glass and metal particles will now collect at the greased part and can easily be removed with a cloth together with the grease. If necessary repeat this procedure. Operating Manual SIGMA 2-16KC, page 72 of 91 10/06...
  • Page 73 Principally we want to point out that for centrifuging of e.g. infectious material certified and hermetically sealed accessories have to be used in order to avoid that the centrifuge is contaminated. Operating Manual SIGMA 2-16KC, page 73 of 91 10/06...
  • Page 74 Rubber cushions 115-118 Round carriers for 13104/ 115-118 13117, Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Round carriers for 13350/ 115-118 13550, Polypropylene Rectangular carriers, 115-118 Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Operating Manual SIGMA 2-16KC, page 74 of 91 10/06...
  • Page 75 50.000 cycles. Any changes like e.g. corrosion, cracks, material abrasion etc. require special attention. 5. Screw connections have to be tight. Furthermore, the earth wire must be checked regularly. Operating Manual SIGMA 2-16KC, page 75 of 91 10/06...
  • Page 76 (exception: curve 0). − Curve 0 decelerates brakeless. Comment: The acceleration time depends on the moment of inertia of the rotor. Linear Curves (0 - 9) Figure 2 Operating Manual SIGMA 2-16KC, page 76 of 91 10/06...
  • Page 77 Slope 240 sec./1000 rpm. 180 sec./1000 rpm. 120 sec./1000 rpm. 60 sec./1000 rpm. 40 sec./1000 rpm. 30 sec./1000 rpm. 20 sec./1000 rpm. 10 sec./1000 rpm. 5 sec./1000 rpm. 0,9 sec./1000 rpm. Operating Manual SIGMA 2-16KC, page 77 of 91 10/06...
  • Page 78 Quadratic curve no. Time up to 1000 rpm Slope from 1000 rpm linear sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. Operating Manual SIGMA 2-16KC, page 78 of 91 10/06...
  • Page 79 If the density of the liquid is higher than 1.2 g/cm , the allowed maximum speed of the centrifuge is calculated according to the following formula: n = n , 1 ( Rho = density in g/cm Operating Manual SIGMA 2-16KC, page 79 of 91 10/06...

  • Page 80: Error Correction

    • These error numbers indicate an error in to 77 after power on. the internal program storage. This error is e.g. generated when a power failure occurs during a storage procedure. Operating Manual SIGMA 2-16KC, page 80 of 91 10/06...

  • Page 81: Emergency Lid Release

    After an emergency release the lid lock needs double the time to close once only. 9.5.5 Problems with the Centrifuge? Please contact your supplier for support or in the event of malfunctions and for supply of spare parts. Operating Manual SIGMA 2-16KC, page 81 of 91 10/06...

  • Page 82: Error Codes

    • check rotor and set rotor Incorrect rotor resp. bucket identification Should it not be possible to repair the failure, please contact Service. Speed-RCF-Diagram An additional help is the enclosed Speed-RCF-Diagram. Operating Manual SIGMA 2-16KC, page 82 of 91 10/06...

  • Page 83: Declaration Of Decontamination / Return Declaration

    Fill in the forms and attach them when returning centrifuges, accessories and spare parts. Please understand that we cannot carry out any work before we have the declarations. (We recommend to make several copies of this page.) Operating Manual SIGMA 2-16KC, page 83 of 91 10/06...
  • Page 84 Operating Manual SIGMA 2-16KC, page 91 of 91 10/06...

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