Page 1
Translation of the original operating manual 08/2012 Rev. 1.24 of 28/04/2022 Sigma 2-16KL; ; part no. 0700702 Sigma 2-16KL from serial no. 146109 Refrigerated Centrifuge Operating Manual Please retain for later use!
Page 2
Refrigerated Centrifuge Sigma 2-16KL Version 08/2012, Rev. 1.24 of 28/04/2022 • sb 2 / 82 Translation of the original operating manual, part no. 0700702...
Page 4
Refrigerated Centrifuge Sigma 2-16KL Version 08/2012, Rev. 1.24 of 28/04/2022 • sb 4 / 82 Translation of the original operating manual, part no. 0700702...
Refrigerated Centrifuge Sigma 2-16KL Table of contents General information ......................9 Importance of the operating manual .................. 9 Intended use ........................9 Warranty and liability......................9 Copyright ........................10 Standards and regulations ....................10 Scope of supply ......................10 Layout and mode of operation ..................11 Layout of the centrifuge ....................
Page 6
Refrigerated Centrifuge Sigma 2-16KL Table of contents Storage and transport ....................... 26 Dimensions and weight ....................26 Storage conditions ......................26 Notes on transport ......................26 Packaging ........................27 Transport safety device ....................27 Set-up and connection ...................... 28 Installation site ........................ 28 Power supply ........................
Page 7
Refrigerated Centrifuge Sigma 2-16KL Table of contents Malfunctions and error correction ..................49 General malfunctions ...................... 49 7.1.1 Emergency lid release ....................50 Table of error codes ......................51 Service contact ....................... 52 Maintenance and service ....................53 Maintenance ........................53 8.1.1...
Page 8
Refrigerated Centrifuge Sigma 2-16KL Table of contents Version 08/2012, Rev. 1.24 of 28/04/2022 • sb 8 / 82 Translation of the original operating manual, part no. 0700702...
Pos : 32 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030 Sic her hei t======================================= @ 26\m od_1405319007254_68.docx @ 191877 @ 1 @ 1 → Safety Pos : 33 /100 Sigma/110 BA Zentrifugen Sigma (Pr ojekte) /2- 16KL_2-16KH L/030 Sic her hei t/030-0010 Bes chil der ung des Geräts 2-16KL @ 54\mod_1513239139922_68.docx @ 541078 @ 2 @ 1 → Marking of the unit...
Refrigerated Centrifuge Sigma 2-16KL 3 Safety Pos : 35 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0020 Symbol- und Hi nweis erklär ung en @ 26\mod_1405319013955_68.docx @ 191947 @ 2 @ 1 → Explanation of the symbols and notes...
Refrigerated Centrifuge Sigma 2-16KL 3 Safety Pos : 37 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0030 Ver antw ortung des Betrei bers @ 26\mod_1405319015364_68.docx @ 191961 @ 2 @ 1 → Responsibility of the operator The operator is responsible for authorising only qualified personnel to work on the centrifuge (see chapter 3.4 - "Requirements concerning the...
Refrigerated Centrifuge Sigma 2-16KL 3 Safety Pos : 39 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0040 Pers onalanforderungen @ 26\mod_1405319017634_68.doc x @ 191989 @ 2 @ 1 → Requirements concerning the personnel Risk of injury if the personnel are not sufficiently qualified...
Refrigerated Centrifuge Sigma 2-16KL 3 Safety Pos : 41 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0050 Informelle Sic herheits hinw eise @ 26\m od_1405319019966_68.docx @ 192017 @ 2 @ 1 → Informal safety instructions • This operating manual is a part of the product.
Leerz eile @ 0\m od_1202116244500_0.doc x @ 114 @ @ 1 Pos : 46 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0020 M ec hanisc he Sic her heit (auß er 1-16/1-16K/1-7) @ 26\mod_1405319024314_68.docx @ 192059 @ 3 @ 1 →...
Leerz eile @ 0\m od_1202116244500_0.doc x @ 114 @ @ 1 Pos : 50 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0040 C hemische und bi ologisc he Sic her hei t @ 26\m od_1405319028141_68.docx @ 192101 @ 3 @ 1 →...
Refrigerated Centrifuge Sigma 2-16KL 3 Safety Pos : 52 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0050 Sicherheits hi nweis e z ur Z entrifug ation @ 26\mod_1405319029632_68.doc x @ 192115 @ 3 @ 1 → 3.6.5 Safety instructions for centrifugation For safe operation, observe the following before starting the centrifuge: •...
Refrigerated Centrifuge Sigma 2-16KL 3 Safety Pos : 56 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0063 Lebensdauer M asc hRL - Übersc hrift neutral @ 83\mod_1622527518082_68.docx @ 878477 @ 3 @ 1 3.6.7 Service life Pos : 57 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0064 Lebensdauer M asc hRL - T ext @ 26\mod_1405319032612_68.doc x @ 192143 @ @ 1 The rotors and accessories have a limited service life.
Refrigerated Centrifuge Sigma 2-16KL 3 Safety Pos : 74 /100 Sigma/100 BA Zentrifugen Sigma (Stan dar dmodule)/030 Sic her heit/030-0080 Ver halten bei Gefahren und Unfäll en @ 27\mod_1405319051188_68.docx @ 192325 @ 2 @ 1 → Measures in the event of hazards and accidents •...
→ Power supply Pos : 96 /100 Sigma/100 BA Zentrifugen Sig ma (Standar dmodule)/050 Aufstellung und Ans chl uss/050- 0020-0011 Ansc hl uss art (Kaltger ätesteck er+Sc hutzsc halter) @ 32\mod_1417600691402_68.docx @ 222498 @ 3 @ 1 5.2.1 Type of connection...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 111 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/060- 0020-0020-0021 Eins etzen eines Wi nk elrotors mit H ermetikdeck el 3 Nm @ 29 \mod_1405321531516_68.docx @ 197015 @ 4 @ 1 →...
Pos : 115 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Be trieb_Spi nc ontr ol L+S/060- 0020-0020-0030 Eins etzen v on Z ubehör 4plätzig 2-16P, 2-16KL, 3- 16L, 3- 16KL, 3-18KS @ 63\mod_1554803016350_68.doc...
Page 34
Refrigerated Centrifuge Sigma 2-16KL 6 Using the centrifuge Centrifugation with low capacity • Install the sample vessels in a rotationally symmetrical manner so that the buckets and their suspensions are loaded evenly. • It is not permissible to load angle rotors on only one axis.
6 Using the centrifuge Pos : 121 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol L (Z ent6) /060- 0030 Spincontr ol L Steuer ung---------------------------------------------- @ 27\m od_1405319249442_68.docx @ 192605 @ 2 @ 1 →...
Pos : 129 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol L (Z ent6) /060- 0030-0020- 0030 U nterbr ec hen eines Brems vorgangs @ 27\m od_1405319258018_68.docx @ 192689 @ 4 @ 1...
6 Using the centrifuge Pos : 133 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol L (Z ent6) /060- 0030-0020- 0050 Drehz ahl/RZB @ 27\m od_1405319260646_68.docx @ 192717 @ 4 @ 1 →...
Page 39
Refrigerated Centrifuge Sigma 2-16KL 6 Using the centrifuge Runtime as of the set speed If the runtime is to be counted as of the moment when the set speed is reached, the symbol (see the illustration) behind the set speed value must be activated: •...
Pos : 137 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol L (Z ent6) /060- 0030-0020- 0070 T em per atur - nur KL @ 27\m od_1405319266022_68.docx @ 192773 @ 4 @ 1...
Page 41
Refrigerated Centrifuge Sigma 2-16KL 6 Using the centrifuge Program "Rapid Temp" The centrifuge has a special "Rapid Temp" program that precools the centrifuge rapidly under defined conditions. • Select the program with the aid of the menu item "run Prog" (see chapter 6.3.3.2 - "Loading a program").
6 Using the centrifuge Pos : 145 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol L (Z ent6) /060- 0030-0020- 0110 Ak ustisc hes Signal @ 27\m od_1405319286360_68.docx @ 192885 @ 4 @ 1 →...
6 Using the centrifuge Pos : 149 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol L (Z ent6) /060- 0030-0020- 0130 Z yklenanz eige @ 27\mod_1405319298959_68.docx @ 192941 @ 4 @ 1 6.3.2.13 Cycle display ("Cycles") In order to activate the cycle display: •...
6 Using the centrifuge Pos : 156 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol L (Z ent6) /060- 0030-0030- 0010 Programm speic her n @ 27\m od_1405319309781_68.docx @ 193025 @ 4 @ 1 →...
Malfunctions and error correction Pos : 164 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/0 70 Störungen und F ehl ersuche/070-0015 Allgemei ne Stör ung en_Spi ncontr ol L @ 28\m od_1405320581367_68.docx @ 194733 @ 2 @ 1 →...
7 Malfunctions and error correction Pos : 166 /100 Sigma/110 BA Z entrifugen Sigma (Proj ekte)/2-16KL_2- 16KH L/070 Störungen und F ehl ers uche/070- 0010-0010 N otentriegel ung 2-16KL_KH L @ 29\mod_1405321449066_68.doc x @ 196777 @ 3 @ 1 7.1.1...
Refrigerated Centrifuge Sigma 2-16KL 7 Malfunctions and error correction Pos : 168 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/070 Störungen und F ehl ersuche/070-0020 T abell e der F ehlerm eldung en @ 28\mod_1405320582996_68.docx @ 194761 @ 2 @ 1 →...
Refrigerated Centrifuge Sigma 2-16KL 8 Maintenance and service Pos : 173 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Inst andhaltung/080 Wartung und Instandhaltung========================= @ 28\m od_1405320612731_68.docx @ 194789 @ 1 @ 1 → Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress.
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 192 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0020 Sterilisati on und Desinfek tion v on Rotork ammer und Zubehör @ 28\m od_1405320626090_68.docx @ 194929 @ 2 @ 1 →...
Refrigerated Centrifuge Sigma 2-16KL 8 Maintenance and service Pos : 194 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0020- 0010 Autoklavi eren @ 28\mod_1405320627440_68.doc x @ 194943 @ 3 @ 1 → 8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use.
Refrigerated Centrifuge Sigma 2-16KL 8 Maintenance and service Pos : 196 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0030 Ins tandhaltungs arbeiten @ 28\mod_1405320628810_68.docx @ 194957 @ 2 @ 1 → Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury.
Refrigerated Centrifuge Sigma 2-16KL 8 Maintenance and service Pos : 198 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0040 R ücks endung defekter T eile @ 28\mod_1405320630226_68.doc x @ 194971 @ 2 @ 1 →...
Page 61
NOTE The forms can be downloaded online from www.sigma-zentrifugen.de → [Service] → [Overhaul and repair]. Pos : 199 /010 Univ ers almodul e/ Abs chnittswec hs el @ 0\mod_1202124514062_0.docx @ 418 @ @ 1 Pos : 200 /010 Univ ers almodul e/ Seitenwechs el @ 0\mod_1202116244312_0.docx @ 105 @ @ 1...
Pos : 201 /100 Sigma/1 00 BA Z entrifugen Sigma (Standardm odul e)/090 Entsorgung/090 Ents orgung===================================== @ 28\m od_1405320658842_68.docx @ 194985 @ 1 @ 1 → Disposal Pos : 202 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/090 Entsorgung/090-0010 Entsorgung der Z entrifuge---------------------------------------------- @ 28\m od_1405320660029_68.docx @ 194999 @ 2 @ 1 → Disposal of the centrifuge In accordance with the directive 2012/19/EU, SIGMA centrifuges are marked with the symbol shown to the left.
10 Technical data Pos : 207 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/100 T ec hnisc he D aten/100 T ec hni sche D aten - Ü bersc hrift ============================= @ 7\mod_1306745782782_68.doc x @ 43932 @ 1 @ 1 →...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 212 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/100 T ec hnisc he D aten/100- 0020 Technisc he Dok um entation @ 28\m od_1405320681369_68.docx @ 195055 @ 2 @ 1 →...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 219 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0010- 0023 R adi en der R otoren (WR+AR+Mikrohämatokritteller) @ 28\mod_1405320703631_68.doc x @ 195125 @ 3 @ 1 11.1.1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below.
Page 66
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix Fig. 32: Microhaematocrit rotor Pos : 220 /010 Univ ers almodul e/ Seitenwechs el @ 0\mod_1202116244312_0.docx @ 105 @ @ 1 Version 08/2012, Rev. 1.24 of 28/04/2022 • sb 66 / 82 Translation of the original operating manual, part no. 0700702...
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix Pos : 223 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0030 Beschl eunigungs- und Bremsk urven Spinc ontrol L @ 28\mod_1405320707842_68.doc x @ 195167 @ 2 @ 1 → 11.3 Acceleration and deceleration curves Linear curves are numbered in the direction of increasing acceleration (from right to left).
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix Pos : 225 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0040 T abell e: Lebensdauer v on R otoren und Z ubehör @ 28\mod_1405320709911_68.docx @ 195195 @ 2 @ 1 → 11.4 Table of the service life of rotors and accessories •...
Page 70
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix Pos : 227 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0050 Beständigkeits tabelle @ 28\mod_1405320711439_68.doc x @ 195209 @ 2 @ 1 → Version 08/2012, Rev. 1.24 of 28/04/2022 • sb 70 / 82...
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix 11.5 Resistance data The data refer to resistance at 20°C. NOTE - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde Acetamide saturated Acetone Acrylonitrile Allyl alcohol...
Page 72
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid Copper sulphate CuSO x 5H Cyclohexanol Decane Diaminoethane —...
Page 73
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl Mercury Methyl acetate Methyl alcohol Methyl benzene Methyl ethyl ketone...
Page 74
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide NaOH Sodium hydroxide NaOH Sodium sulfate Spirits Styrene...
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix Pos : 229 /100 Sigma/110 BA Z entrifugen Sigma (Proj ekte)/2-16KL_2- 16KH L/110 Anhang/110-0060 Konformitäts erkl ärung 2- 16KL_KHL @ 29\m od_1405321475538_68.docx @ 196861 @ 2 @ 1 11.6 EC declaration of conformity Version 08/2012, Rev. 1.24 of 28/04/2022 • sb 75 / 82 Translation of the original operating manual, part no.
Page 76
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix Version 08/2012, Rev. 1.24 of 28/04/2022 • sb 76 / 82 Translation of the original operating manual, part no. 0700702...
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix Declaration of conformity – China RoHS 2 11.7 Version 08/2012, Rev. 1.24 of 28/04/2022 • sb 77 / 82 Translation of the original operating manual, part no. 0700702...
Page 78
Refrigerated Centrifuge Sigma 2-16KL 11 Appendix === Ende der Liste für T extm arke Inhalt === Version 08/2012, Rev. 1.24 of 28/04/2022 • sb 78 / 82 Translation of the original operating manual, part no. 0700702...
Refrigerated Centrifuge Sigma 2-16KL Index 12 Index Changes in colour ........58 Changes in structure ........58 Acceleration ..........43 Chemical and biological safety ....21 Acceleration curves ......43, 68 Chemical resistance of plastic ....55 Accessories, cleaning and care ....54 China RoHS 2 –...
Page 80
Refrigerated Centrifuge Sigma 2-16KL Index Dimensions ..........26 Imbalance monitoring system ....24 Direct hazard to the life and health ..... 16 Importance of the operating manual ..... 9 Directive 2002/96/EC ......... 62 Important information ......... 16 Disinfectants ..........57 Improper loading ........
Page 81
Refrigerated Centrifuge Sigma 2-16KL Index Maximum number of cycles ....... 45 Protection class ........28, 63 Maximum speed for tubes ......35 Measures in the event of hazards and Qualified electrician ........18 accidents ..........25 Quick stop ..........37 Mechanical safety ........
Page 82
Refrigerated Centrifuge Sigma 2-16KL Index Saving a program........47 System check ..........24 Scope of supply ......... 10 System error ........49, 51 Selection, display, and modification of data 37 Serial number ........ 12, 52, 59 Table of error codes ........51 Service ............
Need help?
Do you have a question about the 2-16KL and is the answer not in the manual?
Questions and answers