Related Manuals for Sigma 2-16KC
Summary of Contents for Sigma 2-16KC
- Page 1 Operating Manual Refrigerated Centrifuge 2-16KC from Serial No. 134226 Please retain for later use! Version 03/2010, Rev. 1.7 of 27/01/2012 Translation of the original operating manual...
- Page 3 2-16KC In case of inquiries please state the following number: Serial number: Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Phone +49 (0) 55 22 / 50 07-0 +49 (0) 55 22 / 50 07-12 E-mail: info@sigma-zentrifugen.de...
-
Page 5: Table Of Contents
2-16KC Table of Contents Table of Contents General Information Importance of the Operating Manual ................9 Intended Use......................9 Warranty and Liability ....................9 Copyright........................10 Standards and Regulations ..................10 Scope of Supply ....................... 10 Design of the Centrifuge Overview ........................ - Page 6 2-16KC Table of Contents Set-up and Connection Unpacking the Centrifuge ..................22 Transport Safety Device ................... 22 Installation Site ......................23 Power Supply ......................23 5.4.1 Connection....................23 5.4.2 Fuses......................23 Using the Centrifuge Initial Start-Up......................24 Switching the Centrifuge ON..................24 6.2.1...
- Page 7 2-16KC Table of Contents Maintenance and Service Maintenance......................46 8.1.1 Centrifuge ....................46 8.1.2 Accessories ....................47 8.1.2.1 Plastic Accessories ..............47 8.1.2.2 Aluminium Accessories............... 47 8.1.3 Rotors, Buckets and Multiple Carriers............47 8.1.4 Load-bearing Bolts..................48 8.1.5 Glass Breakage ..................48 8.1.6...
-
Page 9: General Information
They are solely intended for this purpose. Any other use beyond this area of application is regarded as improper use. SIGMA Laborzentrifugen GmbH cannot be held liable for any damage resulting from such improper use. The intended use also includes... -
Page 10: Copyright
2-16KC General Information Copyright The copyright concerning the operating manual remains Sigma Laborzentrifugen GmbH. The operatin manual is solely intended for the operator and their personnel. It includes instructions and information that may not be duplicated, distributet, or communicated in any othe way neither in full nor in parts. -
Page 11: Design Of The Centrifuge Overview
2-16KC Design of the Centrifuge Design of the Centrifuge Overview Function knob Display Lid key Stop key Start key Mains power switch Fig. 2.1: Total view of the centrifuge Name plate (see chapter 2.2) Mains power input Equipotential bonding screw Fig. -
Page 12: Name Plate
2-16KC Design of the Centrifuge Name Plate Manufacturer and registered office Type name Serial number Max. speed Kinetic energy Max. density Nominal voltage Input fuse Symbol for special disposal (see chapter 9) CE mark in accordance with the directive 94/9/EC... -
Page 13: Safety
2-16KC Safety Safety Marking of the Unit International symbols used for SIGMA centrifuges: Gefährliche elektrische Spannung Dangerous voltage Courant haute tension Achtung, Betriebsanleitung lesen Attention, consult instruction manual Attention, consulter mode d'emploi Ein (Netzverbindung) On (Power) Marche (mise sous tension) -
Page 14: Explanation Of The Symbols And Notes
2-16KC Safety Explanation of the Symbols and Notes This operating manual uses the following names and symbols to indicate hazards: This symbol stands for a direct hazard to the life and health of persons. Non-observance of these symbols causes serious health problems up to life-endangering injuries. -
Page 15: Responsibility Of The Operator
2-16KC Safety Responsibility of the Operator The operator is responsible to authorise only qualified personnel to work on the machine (see chapter 3.4 "Operating Personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined. -
Page 16: Safety Instructions
Stop the centrifuge immediately in the event of a malfunction (see chapter 7 "Malfunctions and Error Correction") or contact the service of SIGMA Laborzentrifugen GmbH (see 7.3 "Service Contact"). Ensure that all repairs are performed only by authorized and specialized personnel (see 7.3 "Service Contact"). -
Page 17: Fire Prevention
2-16KC Safety Prior to any start-up, check the centrifuge, rotor, and accessories for signs of damage that can be discerned from the outside. Special attention must be paid to all of the rubber parts (e.g. motor cover, lid seal, adapter) in terms of visible structural changes. -
Page 18: Safety Instructions For Centrifugation
2-16KC Safety 3.6.5 Safety Instructions for Centrifugation For safe operation, observe the following before starting the centrifuge: Ensure that the centrifuge was set up properly (see chapter 5 "Set-up and Connection“). Maintain a safety distance of at least 30 cm (12 inches) around the centrifuge. -
Page 19: Safety Devices
2-16KC Safety If other data concerning the service life are engraved on the rotor or bucket, these data shall apply accordingly. For example, a bucket with the engraving “max cycles = 10,000” has a service life of 10,000 cycles, and a rotor with the engraving “Exp.Date 02/15”... -
Page 20: Ground Wire Check
2-16KC Safety 3.7.4 Ground Wire Check For the ground wire check, there is an equipotential bonding screw on the rear panel of the centrifuge. A ground wire check can be carried out by authorized and specialized personnel using a suitable measuring instrument. -
Page 21: Storage And Transport Dimensions And Weight
2-16KC Storage and Transport Storage and Transport Dimensions and Weight Height: 320 mm Height with opened lid: 700 mm Width: 550 mm Depth: 570 mm Weight: 60 kg Fig. 4.1: Dimensions and weight Storage Conditions The centrifuge can be stored for up to a year without any problems. -
Page 22: Set-Up And Connection Unpacking The Centrifuge
2-16KC Set-up and Connection Set-up and Connection Unpacking the Centrifuge The centrifuge is packaged in a slip-lid box. Remove the upper part of the box. Take out the box containing the accessories. Remove the upper foam cushion. Remove the slip-lid box. -
Page 23: Installation Site
2-16KC Set-up and Connection Installation Site Operate the centrifuge only in closed and dry rooms. All the energy supplied to the centrifuge is converted into heat and emitted to ambient air. Ensure sufficient ventilation. Keep a safety distance of at least 30 cm around the centrifuge so that the vents in the centrifuge remain fully effective. -
Page 24: Using The Centrifuge Initial Start-Up
2-16KC Using the Centrifuge Using the Centrifuge Initial Start-Up Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 "Set-up and Connection"). Switching the Centrifuge ON Press the mains power switch on the right side of the front (see Fig. -
Page 25: Installation Of Angle Rotors With A Hermetically Sealed Lid
2-16KC Using the Centrifuge 6.2.2.2 Installation of Angle Rotors with a Hermetically Sealed Lid Screw the rotor cover onto the rotor and tighten it. Lower the rotor with the cover onto the motor shaft . Insert the rotor tie-down screw into the motor shaft. Tighten the rotor tie-down screw with 5 Nm using the supplied rotor wrench so that the spring washer assembly is compressed tightly. -
Page 26: Adapters
2-16KC Using the Centrifuge Fig. 6.2: Permissible loading of the swing-out rotor with different tube sizes Fig. 6.3: Permissible loading of the swing-out rotor 6.2.2.4 Adapters In order to ensure easy handling, even if vessels of various sizes are used, carrier systems were developed. -
Page 27: Spincontrol Comfort
2-16KC Using the Centrifuge Spincontrol Comfort 6.3.1 Operating Panel 1 Centrifuge display 2 Function key 3 Start key 4 Stop key 5 Lid key Fig. 6.4: Operating Panel The centrifuge is started directly via the operating panel. When the centrifuge is switched on, all of the operating keys and displays will be illuminated for a short time. -
Page 28: Manual Mode
2-16KC Using the Centrifuge 6.3.3 Manual Mode 6.3.3.1 Starting a Centrifugation Run The centrifuge is ready for operation when the start key is illuminated. Press the start key in order to start a centrifugation run. 6.3.3.2 Interrupting a Centrifugation Run Press the stop key in order to interrupt a centrifugation run. -
Page 29: Standard Menu
2-16KC Using the Centrifuge 6.3.3.5 Standard Menu If this area is inverted, the alteration mode is active (here in combination with the speed). Fig. 6.6: "Set" function Speed In the upper section of the field, the set speed of the centrifuge is displayed. - Page 30 2-16KC Using the Centrifuge The set value is indicated in hours, minutes, and seconds. The actual value has the same units as the set value and is displayed in hours:minutes, or in minutes:seconds if the value is below 10 minutes. Values greater than 59 will be automatically converted to the next higher unit.
- Page 31 2-16KC Using the Centrifuge Program In this field, the number of the actual program is displayed. Select the double arrow ( ) next to the program list and confirm the entry. An overview of all stored programs will be displayed (see 6.3.4 "Program Mode").
-
Page 32: Parameter Menu
2-16KC Using the Centrifuge Identifying and adapting incorrectly set rotors The centrifuge automatically identifies the rotor that is being currently used. If the system identifies a different rotor than the one that is set, if there are no different buckets for this rotor, and if the speed has not been adapted manually, the rotor input will be adapted automatically. - Page 33 2-16KC Using the Centrifuge Density If the density of the liquid is higher than 1.2 g/cm , the value must be adapted manually. This, in turn, reduces the maximum final speed (see 11.4.2 “Density“). A downward facing arrow ( ) will be displayed in the speed field.
- Page 34 2-16KC Using the Centrifuge An entry will interrupt the rapid cooling program. There will be no message when the set temperature is reached. If no new runtime is entered, the centrifuge will continue to run infinitely (continuous run). Start Delay If the start delay is activated, the centrifuge will not start until the preset time has elapsed.
- Page 35 2-16KC Using the Centrifuge Unblocking a function Select the button "Delete code". Enter the code and confirm the entry. The function is now unblocked. Changing the code Select the button "Change code". Enter the old code and confirm the entry.
-
Page 36: Curves Menu
2-16KC Using the Centrifuge Cycles For each rotor/bucket combination, the number of cycles and runtime are stored. Buzzer In this menu, a sound signal can be selected for the end of a centrifugation run an imbalance message an error message. - Page 37 2-16KC Using the Centrifuge Creation of the curves for variable accelerations and decelerations In the configuration mode, select the field "Curves" and confirm the selection. The curve overview will be displayed. Select the time display and confirm the selection. Enter the desired time for interval no. 1 and confirm the entry. The display for internal no.
-
Page 38: Modification Of The Contrast
2-16KC Using the Centrifuge Cut: The curve speed in an interval will automatically be limited to the set speed in the operating mode. Admit: The curve speed in an interval will be allowed. Cancel: A stop occurs. Starting is impossible without a change of the curve speed in an interval. -
Page 39: Modification Of Parameter Values During The Centrifugation Run
2-16KC Using the Centrifuge 6.3.3.11 Modification of Parameter Values during the Centrifugation Run (see 6.3.3.4 “Selection, Display, and Modification of Data"): Modification possible Modification (De-)Activation impossible possible Speed Rotor Continuous run Program Standstill cooling Runtime Radius Start delay Temperature Density... -
Page 40: Loading A Program
2-16KC Using the Centrifuge 6.3.4.1 Loading a Program 1. Loading a program by entering a program number Select the program field ("PROG-") and confirm the selection. Turn the knob. As a result, all of the programs that have already been saved and the current ("--") setting will be displayed one after the other. -
Page 41: Deleting A Program
2-16KC Using the Centrifuge 6.3.4.3 Deleting a program Select the double arrow ( ) next to the program selection list and confirm the selection. An overview with all of the programs that have already been saved will be displayed. Select the program that is to be deleted and confirm the selection. -
Page 42: Malfunctions And Error Correction General Malfunctions
2-16KC Malfunctions ans Error Correction Malfunctions and Error Correction General Malfunctions Malfunctions are indicated by a dialogue box. If the sound signal is activated, it sounds when the error message is displayed. Eliminate the source of the problem (see tables in fig. 7.1 and 7.3). -
Page 43: Emergency Lid Release
2-16KC Malfunctions and Error Correction 7.1.1 Emergency Lid Release In the event of a power failure, it is possible to manually open the centrifuge lid. Switch off the mains power switch and disconnect the power cord from the socket. Remove the plug (1) at the right side of the centrifuge, e.g. with a screw driver (see fig. -
Page 44: Error Codes
2-16KC Malfunctions ans Error Correction Error Codes Error no. Kind of error Measure Note System error All these errors stop the centrifuge allow to slow down or cause it to decelerate brakeless power off/on 10-19 Speedometer allow to slow down... -
Page 45: Service Contact
[Service Area] or contact SIGMA Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel. +49 (0) 55 22 / 50 07-84 25 Fax +49 (0) 55 22 / 50 07-94 25 E-mail: service@sigma-zentrifugen.de... -
Page 46: Maintenance And Service
2-16KC Maintenance and Service Maintenance and Service The centrifuge, rotor, and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure. If corrosion or other damage occurs due to improper care, the manufacturer cannot be held liable or subject to any warranty claims. -
Page 47: Accessories
2-16KC Maintenance and Service 8.1.2 Accessories For the care of the accessories, special safety measures must be considered as these are measures that will ensure operational safety at the same time. Immediately rinse off the rotor, buckets, or accessories under running water if they have come into contact with any liquids that may cause corrosion. -
Page 48: Load-Bearing Bolts
2-16KC Maintenance and Service Check the material regularly (at least once a month) for - cracks - visible damage of the surface - pressure marks - signs of corrosion - other changes. Check the bores of the rotors and multiple carriers. -
Page 49: Condenser
2-16KC Maintenance and Service 8.1.6 Condenser In order to cool the refrigerant that is compressed by the refrigeration unit, a lamellar condenser is used. It is cooled by air. Dust and dirt obstruct the cooling flow of air. Dust on condenser pipes and lamellas reduces the heat exchange and thus the performance of the refrigeration unit. -
Page 50: Service
2-16KC Maintenance and Service Accessories max. temp. min. time max. time max. (°C) cycles Glass tubes 134-138 Polycarbonate tubes 115-118 Polypropylene copolymer tubes 115-121 Teflon tubes 134-138 Aluminium rotors 134-138 Polycarbonate/Polyallomer lids for angle rotors 115-118 Polysulfone lids for angle rotors... -
Page 51: Return Of Defective Parts
[Service Area] or contact SIGMA Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel. +49 (0) 55 22 / 50 07-84 25 Fax +49 (0) 55 22 / 50 07-94 25 E-mail: service@sigma-zentrifugen.de... -
Page 52: Disposal
This symbol means that it is not permissible to dispose of the unit among household trash. You can return these centrifuges free of cost to SIGMA Laborzentrifugen GmbH. Ensure that the unit is decontaminated. Fill in a declaration of decontamination. -
Page 53: Technical Data
2-16KC Technical Data Technical Data Manufacturer: S I G M A Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Type: 2-16KC Electr. Connection: see name plate Protection class: IP Code: Connected load (kVA): 1,05 Power consumption (kW): 0,72 Max. -
Page 54: Appendix
2-16KC Appendix Appendix 11.1 Suitable Accessories Part no. Description Max. speed Max. (rpm) gravitational field (x g) 11124 Swing-out rotor 24 x 1.5 / 2.0 ml, incl. 3 sets buckets no. 14 000 16 215 13124, for e.g. reaction vials no. 15008, 15040, incl. - Page 55 2-16KC Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 12073 Angle rotor 30 x 15 ml complete, incl. 15 sets buckets no. 13011, for tubes Ø 17 x up to 120 mm, e.g. no. 15015, 15020, 15023, 15024, Monovettes and culture tubes 15 ml no.
- Page 56 2-16KC Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 17950 Adapter for 1 tube 50 ml no. 15049, 15050, 15056, 1 set = 2 pcs. 13041 Round bucket for round carriers Ø 43.5 mm, no. 14029, 14030, 14031, 14032, 14033, 14034, 14035, incl.
- Page 57 2-16KC Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 11123 Swing-out rotor for microtiter plates, incl. 1 set carriers no. 13223 radius corner 11.9 cm, 4 000 2 129 radius max. 10 cm, 1 789 radius min. 6.5 cm 1 163 max.
-
Page 58: Maximum Speed For Tubes
2-16KC Appendix Part no. Description 15054 Polycarbonate tube 42 ml, Ø 28.8 x 107 mm, incl. screw cap, suitable for 13079 15151 Culture tube Nunc, 50 ml, pointed bottom, polypropylene, incl. screw cap, suitable for 12151, 13150 15102 Polypropylene tube 120 ml, Ø 45 x 100 mm, suitable for 13097 15103 Polycarbonate tube 110 ml, Ø... -
Page 59: Grafical Representation Of The Rotors
2-16KC Appendix 11.3 Grafical Representation of the Rotors The graphical representation of the rotors shows the maximum and minimum radii of the accessories used. If necessary, the values must be manually calculated (see 11.4.1 "RCF“, page 62). Fig 11.1: Minimum and maximum radius of a swing-out rotor Fig. -
Page 60: Acceleration And Deceleration Curves
2-16KC Appendix 11.4 Acceleration and Deceleration Curves 11.3.1 Linear Curves The slope of the fixed acceleration curves defines the time that is required to accelerate the rotor by 1,000 rpm. Linear as well as quadratic curves are numbered in the direction of increasing acceleration (from right to left). -
Page 61: Quadratic Curves
2-16KC Appendix Linear curve no. Slope 4 [rpm / sec] 6 [rpm / sec] 8 [rpm / sec] 17 [rpm / sec] 25 [rpm / sec] 33 [rpm / sec] 50 [rpm / sec] 100 [rpm / sec] 200 [rpm / sec] 1000 [rpm / sec] Fig. -
Page 62: Formulae - Mathematical Relations
2-16KC Appendix Quadratic curve no. Time until 1,000 rpm Slope as of 1,000 rpm 500 sec 4 [rpm / sec] 333 sec 6 [rpm / sec] 250 sec 8 [rpm / sec] 118 sec 17 [rpm / sec] 80 sec... - Page 63 2-16KC Appendix Fig. 11.7: Speed-Gravitational-Field Diagram Version 03/2010, Rev. 1.7 of 27/01/2012 Translation of the original operating manual...
-
Page 64: Table Of Rotors And Accessories With A Different Service Life
2-16KC Appendix Table of rotors and accessories with a different service life Rotors and accessories with a different service life If other no data concerning the service life are engraved on the rotor or accessory, rotors and buckets must be checked by the manufacturer after 10,000 cycles. After 50,000 cycles, rotors must be scrapped for safety reasons. -
Page 65: Resistance Data
2-16KC Appendix Resistance Data Resistance Data Resistant at +20 °C - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde Acetamide saturated Acetone Acrylonitrile Allyl alcohol Aluminum chloride AlCl saturated Aluminum sulfate Ammonium chloride... - Page 66 2-16KC Appendix Resistant at +20 °C - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Dioxane Dipropylene glycol (mono)methyl ether Ethyl acetate Ethylene chloride Ferrous chloride FeCl saturated Formaldehyde solution Formic acid Furfural...
- Page 67 2-16KC Appendix Resistant at +20 °C - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Potassium hydrogen carbonate CHKO saturated Potassium hydroxide Potassium hydroxide Potassium nitrate Potassium permanganate KMnO Pyridine Resorcinol Silver nitrate...
-
Page 69: Ec-Declaration Of Conformity
EC-Declaration of Conformity... -
Page 71: Index
2-16KC Index Index Acceleration........................29, 32 Acceleration curve ..................... 32, 36, 39, 60 Accident prevention ......................15 Acid ........................18, 46, 65, 67 Adapters ........................26, 48, 58 Alkaline solutions ......................18, 47 Aluminium accessories ......................47 Ambient conditions....................... 53 Ambient temperature ...................... - Page 72 2-16KC Index Cost estimate........................52 Cracks ........................18, 47, 48 Creation of the curves for variable accelerations and decelerations ........37 Curves ..........................36 Curves menu ........................36 Cycles........................18, 24, 36 Damage of the surface......................48 Dangerous goods......................... 18 Dangerous materials......................
- Page 73 2-16KC Index Hazard warnings......................9, 24, 25 Heater..........................30, 53 Identifying and adapting incorrectly set rotors ..............32 Imbalance ......................18, 25, 26, 48 Imbalance dialog box ......................38 Imbalance error........................44 Imbalance message......................36 Imbalance monitoring..................... 20, 38 Importance of the operating manual..................9 Important information ......................
- Page 74 2-16KC Index Maximum speed for glass tubes..................26, 58 Maximum speed for tubes....................58 Measures in the event of hazards and accidents..............20 Mechanical safety ........................ 16 Modification of existing curves ..................... 38 Modification of parameter values during the centrifugation run..........39 Modification of the contrast ....................
- Page 75 2-16KC Index Removal of the transport safety device ................22 Remove glass particles and metal dust from the rotor chamber ........... 48 Reset ........................... 36 Resistance data ....................... 18, 47, 65 Resistance of plastics ......................18 Responsibility of the operator....................15 Return of centrifuges, spare parts, or accessories ...............
- Page 76 2-16KC Index Speed field........................27, 33 Speed-Gravitational-Field-Diagram..................62 Speedometer error....................... 44 Spincontrol Comfort ......................27 Spincontrol Professional ...................... 53 Standard menu ........................29 Standards and regulations ....................10 Standstill cooling......................33, 39 Standstill monitoring......................19 Start delay........................34, 39 Start of the centrifuge......................29 Start the continuous run.......................
- Page 77 2-16KC Short Operating Instructions Pay attention to the detailed Operating Manual, especially to the safety instructions in chapter 3! Function knob Display Lid key Stop key Start key Mains power switch Total view of the centrifuge Name plate Mains power input...
- Page 78 2-16KC Short Operating Instructions Enter run parameters: Manual Mode • Select a parameter by pressing or turning the function knob. The selected field will be inverted. • Press the function knob in order to activate the modification mode. "SET" and the selected area will be inverted.