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Summary of Contents for Sigma 2-16P
- Page 1 Laboratory Centrifuge 2-16P Operating Manual Please retain for later use!
- Page 3 Dear Customer, Congratulations on your purchase of a SIGMA laboratory centrifuge. You have selected a device that combines many advantages. The SIGMA 2-16P is a microprocessor-controlled universal laboratory centrifuge. The electronic operation control enables a trouble-free use of the centrifuge. With its quiet 3- phase maintenance-free drive, operation without any carbon dust pollution is guaranteed.
- Page 5 EN 61000-3-2:2006; EN 61000-3-3:1995, A2:2005 Normes : EN 61326-1:2006 Sigma Laborzentrifugen An der Unteren Söse 50 D-37520 Osterode 23.11.2007 Geschäftsführer Managing Director Directeur Gérant ................ Fabr. Nr. Serial No. Numéro de série 2-16 P 20071123.DOC Operating Manual SIGMA 2-16P page 5 of 75 04/2007...
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Page 7: Table Of Contents
Dimensions and Weight Notes on Transport Notes on Storage Set-up and Connection Unpacking the Centrifuge 4.1.1 Transport Safety Device Installation 4.2.1 Installation Site 4.2.2 Connection 4.2.3 Fuses/Emergency Circuit Breaker on Site Operating Manual SIGMA 2-16P page 7 of 75 04/2007... - Page 8 5.2.7.1 Interrupting a Deceleration Process 5.2.7.2 Softstart and Softstop Function Display / Program Options 5.3.1 Time 5.3.1.1 Changing the Time Increments 5.3.1.2 Short Run 5.3.1.3 Continuous Run 5.3.2 Speed 5.3.2.1 Changing the Speed Increments Operating Manual SIGMA 2-16P page 8 of 75 04/2007...
- Page 9 7.1.3 Rotor, Buckets, and Multiple Carriers 7.1.3.1 Microhematocrite Rotor 7.1.4 Load-bearing Bolts 7.1.5 Glass Breakage Sterilization and Disinfection of the Rotor Chamber and Accessories 7.2.1 Autoclaving Disposal Disposal of the Centrifuge Disposal of the Packaging Operating Manual SIGMA 2-16P page 9 of 75 04/2007...
- Page 10 10.1 Graphical Representation of the Rotors 11 Appendix 11.1 Formulae – Mathematical Relations 11.1.1 Relative Centrifugal Force (RCF) 11.1.2 Density 11.1.3 Speed-Gravitational-Field-Diagram 11.2 Declaration of Decontamination/Return Declaration 11.3 Resistance Data 12 Index Operating Manual SIGMA 2-16P page 10 of 75 04/2007...
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Page 11: General Information
(see BGR 500, chapter 2.11, part 3). They are solely intended for this purpose. Any other use beyond this area of application is regarded as improper use. SIGMA Laborzentrifugen GmbH cannot be held liable for any damage resulting from such improper use. -
Page 13: Technical Data
220-240 V +/- 5%. • Allowable ambient temperature +4°C to +40°C. • Max. humidity 80%. • Lowest storage and transport temperature –20°C (see chapter 3 "Transport and Storage", page 22). Operating Manual SIGMA 2-16P page 13 of 75 04/2007... -
Page 15: Scope Of Supply
• Equipment Decontamination Certificate (page 67) Accessories according to your order, our order confirmation, and our delivery note. Standards and Regulations Please refer to the enclosed EU Declaration of Conformity (page 5). Operating Manual SIGMA 2-16P page 15 of 75 04/2007... -
Page 16: Safety Instructions And Hazard Warnings
Hot surface Surface chaude Nicht mit dem Hausmüll entsorgen Do not dispose as part of domestic waste Ne pas jeter avec les déchets ménager Fig. 2.1: Symbols used for SIGMA centrifuges Operating Manual SIGMA 2-16P page 16 of 75 04/2007... -
Page 17: Symbols Used In The Operating Manual
The operating personnel must have read and understood the operating manual prior to operation. • Please also comply with the general and company-specific rules and regulations for the prevention of accidents. Operating Manual SIGMA 2-16P page 17 of 75 04/2007... -
Page 18: Safety Instructions For Centrifugation
When not using the centrifuge, open the lid so that all liquids can evaporate. • Stop the centrifuge immediately in the event of a malfunction. Eliminate the problem (see 6.2, page 44 ff) or inform the SIGMA Laborzentrifugen GmbH service team (see 6.2.7 "Service Contact", page 46). Operating Manual SIGMA 2-16P... -
Page 19: Resistance Of Plastics
Only use the centrifuge with rotors and accessories that have been approved by the manufacturer. We explicitly warn against the use of equipment of poor quality. Breaking glass or bursting vessels can cause dangerous imbalances at high speeds. Operating Manual SIGMA 2-16P page 19 of 75 04/2007... -
Page 20: Special Hazards
Do not use the centrifuge within hazardous locations. • Do not spin explosive or inflammable substances. • Materials that chemically react with each other with a high level of energy are prohibited. Operating Manual SIGMA 2-16P page 20 of 75 04/2007... -
Page 21: Checks By The Operator
Use the centrifuge only for the purpose that it was originally intended for (see 1.2, page 11). • Use the centrifuge only if it is in a perfect running state. • Immediately eliminate any problems that can affect safety. Operating Manual SIGMA 2-16P page 21 of 75 04/2007... -
Page 22: Transport And Storage
The storage temperature must not exceed –20°C. • If you would like to store it for more than one year, or if you intend to ship it overseas, please contact the manufacturer. Operating Manual SIGMA 2-16P page 22 of 75 04/2007... -
Page 23: Set-Up And Connection
Please retain the packaging for any possible future transport of the centrifuge. 4.1.1 Transport Safety Device The transport safety device of the SIGMA 2-16K centrifuge consists of a foamed plastic ring in the centrifuge chamber. It must be removed prior to start-up. Procedure: •... -
Page 24: Installation
The operating voltage on the name plate must correspond to the local supply voltage! SIGMA laboratory centrifuges are units of safety class I in accordance with DIN VDE 0700 and have a three-wire power cord (2.5 m) and a shockproof right- angle plug. -
Page 25: Using The Centrifuge
Display Start key Left rotary knob Right rotary knob Fig. 5.1: Total view of the centrifuge Name plate Equipotential bonding screw Mains power input Fig. 5.2: Rear view of the centrifuge Operating Manual SIGMA 2-16P page 25 of 75 04/2007... -
Page 26: Operating Panel
Nominal voltage Input fuse Symbol for special disposal (see chapter 8, page 51) CE mark in accordance with the directive 94/9/EC Part number Year of manufacture Power consumption Fig. 5.4: Name plate Operating Manual SIGMA 2-16P page 26 of 75 04/2007... -
Page 27: Construction And Constructive Safety Measures
Relative centrifugal force RCF in steps of 1 or 10 x g • Time between 10 sec an 11h 59min max. • Continuous run • Short run • Rotor preselection • Acceleration and deceleration curves • Storage and call-up of programs Operating Manual SIGMA 2-16P page 27 of 75 04/2007... -
Page 28: Safety Devices
"Imbalance" and the error number 46 for "Imbalance“ will then flash on the display. The lid opens automatically after the rotor has come to a standstill. In both cases, loading has to be checked and balanced. Operating Manual SIGMA 2-16P page 28 of 75 04/2007... -
Page 29: Initial Start-Up
To close the lid, press on the left and right side of the lid to ensure that both of the lid locks lock into place. Attention! A flashing lid key indicates that only one lid lock has locked. Operating Manual SIGMA 2-16P page 29 of 75 04/2007... -
Page 30: Installation Of Rotors
Prior to installing the lid, check for the tight fit (5 Nm) of the rotor tie-down screw. Attention! • Please follow the safety instructions and hazard warnings in chapter 2, page 16 ff! Operating Manual SIGMA 2-16P page 30 of 75 04/2007... -
Page 31: Installation Of Angle Rotors With A Hermetically Sealed Lid
Please follow the safety instructions and hazard warnings in chapter 2, page 16 ff! 1 Rotor tie-down screw 2 Lid 3 Lid seals Fig. 5.5: Angle rotor with a hermetically sealed lid Operating Manual SIGMA 2-16P page 31 of 75 04/2007... -
Page 32: Installation Of The Microhematocrite Rotor
• Remove the capillary tubes. Some of the tubes can also be evaluated with the reader outside of the rotor (see the instructions for use on the back of the reader). Operating Manual SIGMA 2-16P page 32 of 75 04/2007... -
Page 33: Installation Of Accessories
Another example is the angle rotor 24 x 2.2 ml: The tubes must be installed symmetrically so that the rotor is loaded evenly. Fig. 5.8: Permissible loading of the angle rotor Operating Manual SIGMA 2-16P page 33 of 75 04/2007... -
Page 34: Carrier Systems
After 50,000 cycles, the rotor must be scrapped for reasons of safety. • If other data concerning the service life are engraved on the rotor or bucket, these data shall apply accordingly! Operating Manual SIGMA 2-16P page 34 of 75 04/2007... -
Page 35: Starting The Centrifuge
• Press the key five times to activate the “brakeless deceleration” function alone (the “soft stop” display flashes). • Press the key once more to return to the standard operating mode. Operating Manual SIGMA 2-16P page 35 of 75 04/2007... -
Page 36: Display / Program Options
If the centrifugation time is changed during the run, the centrifuge will run for the entire new time and will disregard the previous runtime that has already elapsed. Fig. 5.10: Setting the time Operating Manual SIGMA 2-16P page 36 of 75 04/2007... -
Page 37: Changing The Time Increments
Starting with the time setting 11:59, turn the knob clockwise to the next setting. The message “cont” and the symbol "∞" will be displayed (Fig. 5.12). Fig. 5.12: Continuous run display Operating Manual SIGMA 2-16P page 37 of 75 04/2007... -
Page 38: Speed
In order to change the speed in steps of 1 rpm (instead of in steps of 100 rpm): • Keep the stop key pressed while setting the desired speed with the right rotary knob. Operating Manual SIGMA 2-16P page 38 of 75 04/2007... -
Page 39: Relative Centrifugal Force (Rcf)
In order to change the RCF value in Stepps of 1 x g (instead of in Stepps of 10 x • Keep the stop key pressed while setting the desired RCF value with the right rotary knob. Operating Manual SIGMA 2-16P page 39 of 75 04/2007... -
Page 40: Rotor Preselection
5.3.4 Rotor Preselection The centrifuge SIGMA 2-16P is equipped with an automatic rotor identification system. After the installation of the rotor, the rotor ID will be displayed. It is also possible to manually preselect the rotor. This is only possible at a standstill. -
Page 41: Program
Turn the right rotary knob until the desired program number is displayed. • Press the right rotary knob to confirm the input. The saved program settings will be adopted. Fig. 5.17: Calling up stored programs; here program number 8 Operating Manual SIGMA 2-16P page 41 of 75 04/2007... -
Page 42: Lockdown
Proceed in the same way in order to deactivate the function. The current status remains active even if the centrifuge is switched off with the help of the mains power switch. Fig. 5.18: Padlock symbol Operating Manual SIGMA 2-16P page 42 of 75 04/2007... -
Page 43: Activating/Deactivating The Automatic Lid Opening Function
Proceed in the same way in order to deactivate the function. After every change, the current status of the setting is displayed in the form of a message running in the display ("Buzzer on" or "off"). Operating Manual SIGMA 2-16P page 43 of 75 04/2007... -
Page 44: Malfunctions And Error Correction
61 • Power off/on (see 6.2.6 "Error Codes", − Centrifuge displays an error from page 46). If the error occurs again, call 1 to 11 after powering on. the service. Operating Manual SIGMA 2-16P page 44 of 75 04/2007... -
Page 45: Lid Cannot Be Opened
The lid may only be unlocked and opened when the rotor is at a standstill. If the lid is opened by way of the emergency lid release during operation, the centrifuge will be switched off immediately and decelerate brakeless. Operating Manual SIGMA 2-16P page 45 of 75 04/2007... -
Page 46: Error Codes
Please contact your supplier for support or in the event of malfunctions and the supply of spare parts: SIGMA Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode Service +49 55 22 50 07-8425 service@sigma-zentrifugen.de Zentrale +49 55 22 50 07-0 info@sigma-zentrifugen.de Operating Manual SIGMA 2-16P page 46 of 75 04/2007... -
Page 47: Care And Maintenance
Grease the motor shaft slightly after cleaning (grease for load-bearing bolts part no. 70284). • Open the centrifuge when it is not in use so moisture can evaporate. This prevents the increased wear of the motor bearings. Operating Manual SIGMA 2-16P page 47 of 75 04/2007... -
Page 48: Accessories
(see 7.1 "Cleaning and Care", page 47). • Grease aluminum parts at least once a week with slushing oil (part no. 70104). This essentially increases their service life and reduces susceptibility to corrosion. Operating Manual SIGMA 2-16P page 48 of 75 04/2007... -
Page 49: Rotor, Buckets, And Multiple Carriers
Remove the microhematrocrite rotor for cleaning. • Wipe the centrifuge chamber clean. • Replace the rubber ring in the event of wear or glass breakage (part no. 16001 for rotor 11409). Operating Manual SIGMA 2-16P page 49 of 75 04/2007... -
Page 50: Load-Bearing Bolts
2,000 rpm). The glass and metal particles will now collect at the greased part and can easily be removed with a cloth together with the grease. • Remove the grease with glass and metal particles with a cloth. • If necessary, repeat this procedure. Operating Manual SIGMA 2-16P page 50 of 75 04/2007... -
Page 51: Sterilization And Disinfection Of The Rotor Chamber And Accessories
(see 7.2.1 “Autoclaving“, page 51). Please contact us if you have any queries (see 6.2.7 "Service Contact", page 46). If dangerous materials (e.g. infectious and pathogenic substances) are used, the centrifuge and accessories must be disinfected. Operating Manual SIGMA 2-16P page 51 of 75 04/2007... -
Page 52: Autoclaving
115-118 Round carriers made of 115-118 polypropylene ditto, made of polyallomer 115-118 and polycarbonate Rectangular carriers made of 115-118 polypropylene ditto, made of polyallomer 115-118 and polycarbonate Fig. 7.1: Autoclaving table Operating Manual SIGMA 2-16P page 52 of 75 04/2007... -
Page 53: Disposal
Disposal Disposal of the Centrifuge In accordance with the directive 2002/96/EC, SIGMA centrifuges are marked with the symbol shown to the left. This symbol means that it is not permissible to dispose of the unit among household trash. • You can return these centrifuges free of cost to SIGMA Laborzentrifugen GmbH. -
Page 54: Suitable Accessories
∅ 17 x 120 mm, e.g. 15015, 15020, 15023, 15024, Monovettes, culture tubes 15 ml 15115, max. radius 13.9 cm, 4 000 2 486 min. radius 8 cm, angle 33° Operating Manual SIGMA 2-16P page 54 of 75 04/2007... - Page 55 16015, max. radius 13.4 cm, min. radius 5.1 cm 4 500 3 034 11192 Swing-out rotor for 4 buckets or multiple carriers 13009, 13012, 13041, 13042, 13097, 13150, 13152 4 500 3 056/ 3 034 Operating Manual SIGMA 2-16P page 55 of 75 04/2007...
- Page 56 15015, 15024, 15039, polyethylene, suitable for 13041 Round carrier for 1 glass tube 25 ml, max. ∅ 14031 25/30 x 70 - 105 mm, e.g. 15025, 15026, 15033, polypropylene, suitable for 13041 Operating Manual SIGMA 2-16P page 56 of 75 04/2007...
- Page 57 Adapter for reaction vials 0.25-0.4 ml 15014, suitable for 11124, 12133, 12148, polyallomer 13002 Adapter for reaction vials 0.5-0.75 ml 15005, Ø 7.9/10 x 28/31 mm, suitable for 11124, 12133, 12148, polyallomer Operating Manual SIGMA 2-16P page 57 of 75 04/2007...
- Page 58 Polycarbonate tube 50 ml, graduated 0 - 50 ml 15049 in steps of 1 ml, ∅ 34 x 100 mm, suitable for 17950 Teflon tube with screw cap 42 ml, ∅ 28.5 x 107 15051 mm, suitable for 13079 Operating Manual SIGMA 2-16P page 58 of 75 04/2007...
- Page 59 Centrifuge glass tube 100 ml, ∅ 44 x 100 mm, 15100 suitable for 11190, 13097 15106 ditto, graduated, 1 - 100 ml in steps of 1 ml Further accessories available upon request. Operating Manual SIGMA 2-16P page 59 of 75 04/2007...
- Page 60 • Always fill the tubes up to their useful volume (= the volume that is stated for the tube). • Follow the manufacturer’s recommendations. Operating Manual SIGMA 2-16P page 60 of 75 04/2007...
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Page 61: Graphical Representation Of The Rotors
If necessary, the values must be manually calculated (see 11.1.1 “RCF”, page 62). Fig. 10.1: Minimum and maximum radius of a swing-out rotor Fig. 10.2: Minimum and maximum radius of an angle rotor Fig. 10.3: Microhematocrite rotor Operating Manual SIGMA 2-16P page 61 of 75 04/2007... -
Page 62: Appendix
. 1 ( n = n Rho = density in g/cm 11.1.3 Speed-Gravitational-Field-Diagram Additional help can be found in the enclosed speed-gravitational-field-diagram (page 63). Operating Manual SIGMA 2-16P page 62 of 75 04/2007... - Page 63 Fig. 11.1: Speed-gravitational-field-diagram Operating Manual SIGMA 2-16P page 63 of 75 04/2007...
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Page 64: Declaration Of Decontamination/Return Declaration
Fill out the forms and attach them when returning centrifuges, accessories, and spare parts. Please understand that we cannot carry out any work before we have the declarations. We recommend several copies of this page to be made. Operating Manual SIGMA 2-16P page 64 of 75 04/2007... - Page 65 !!! Note – this form must be glued onto the outside of the box !!! Return Declaration Decontamination declaration inside: Unit / component contaminated: Unit / component unused: Thank you very much for a brief problem description. This may reduce the repair costs. Please make some copies before removing this page! Fig.
- Page 67 Declaration of Decontamination by the Operator Concerning the contamination of centrifuges, accessories, and spare parts. This declaration may only be filled out and signed by authorized staff. Repair order no. Date Type of unit Serial no. Year Type of unit Serial no.
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Page 69: Resistance Data
Chromic acid Chromic potassium sulfate KCr(SO x 12H saturated Citric acid Citric acid Copper sulfate CuSO x 5H Cyclohexanol Decane Diaminoethane Diesel fuel — Dimethyl formamide (DMF) Dimethyl sulfoxide (DMSO) Dimethylaniline Dioxane Operating Manual SIGMA 2-16P page 69 of 75 04/2007... - Page 70 Mineral oil — Nitric acid Nitric acid Nitrobenzene Oleic acid Oxalic acid x 2H Ozone Petroleum — Phenol Phenol Phosphoric acid Phosphorus pentachloride Potassium hydrogen carbonate CHKO saturated Potassium hydroxide Potassium hydroxide Operating Manual SIGMA 2-16P page 70 of 75 04/2007...
- Page 71 Sulfuric acid Sulfuric acid fuming Tallow — Tetrahydrofuran (THF) Tetrahydronaphthalene Thionyl chloride Transformer oil — Trichloroethane Urea Urine — Vinegar Vinegar — Wines — Xylene Zinc chloride SnCl Fig. 11.4: Resistance data Operating Manual SIGMA 2-16P page 71 of 75 04/2007...
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Page 73: Index
Emergency circuit breaker......................24 Equipotential bonding screw ....................28 Error messages ........................28, 44 Errors..........................28, 46 Fire-fighting measures......................21 Fuse ..........................24, 44 General Conditions.........................53 Glass particles........................50 Gravitational field....................13, 32, 49, 62 Grease for load-bearing bolts..................15, 47, 49 Operating Manual SIGMA 2-16P page 73 of 75 04/2007... - Page 74 Rotor tie-down screw....................30, 31, 32, 49 Rotor wrench ......................15, 30, 31, 32 Rotors for microtiter plate formats ..................30 Rubber cushions ....................48, 49, 50, 52 Safety class ..........................24 Safety distance.........................19, 24 Safety instructions ......................11, 16, 18 Operating Manual SIGMA 2-16P page 74 of 75 04/2007...
- Page 75 Spare parts........................46, 64 Storage temperature ......................13, 22 Stress-corrosion ........................49 Supply voltage........................24 Swing-out rotor ........................33 Toxic substances....................18, 20, 47, 48 Transport safety device ....................22, 23 Trunnion pins..........................21 UV radiation........................24, 47 Ventilation.........................24, 46 Operating Manual SIGMA 2-16P page 75 of 75 04/2007...