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Sigma 2-5 Operating Instruction

Benchtop centrifue
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  • Page 3 Dear customer, Congratulations for purchasing a SIGMA laboratory centrifuge. You have selected a device which combines many advantages. The electronic operation control allows a trouble-free use of the centrifuge. With its 3-phase drive, maintenance-free quiet operation without any carbon dust pollution is guaranteed.
  • Page 5 EN 61000-3-2 ; EN 61000-3-3 Normes : EN 61326 Sigma Laborzentrifugen An der Unteren Söse 50 D-37520 Osterode 01.02.2002 Geschäftsführer Managing Director Directeur Gérant ................ Fabr. Nr. Serial No. Numéro de fabrique Operating Manual SIGMA 2-5, page 5 of 55 03/06...

  • Page 7: General Information

    Switching on of the centrifuge 3.4.2 Opening lid 3.4.3 Installation of an angle rotor and a swing-out rotor Operating Elements Operating panel 4.1.1 Start-key 4.1.2 Stop-key with soft and softstop function 4.1.3 Lid-key 4.1.4 Knobs Operating Manual SIGMA 2-5, page 7 of 55 03/06...

  • Page 8: Care And Maintenance

    Centrifuge decelerates during operation 7.2.3 Lid cannot be opened/closed 7.2.4 Emergency lid release 7.2.5 Problems with the centrifuge? Error mode 7.3.1 Error codes Diagram speed/gravitational field Declaration of decontamination/Return declaration Leaflet Operating Manual SIGMA 2-5, page 8 of 55 03/06...

  • Page 9: Technical Data

    The figures are valid for an ambient temperature of 23 ° C +/- 2 ° C and nominal voltage 220 – 240 volt +/- 5 %. (Allowable ambient temperature +4 ° C - +40 ° C; max. humidity 80 %.) Subject to technical alterations. Operating Manual SIGMA 2-5, page 9 of 55 03/06...
  • Page 11 5-15 ml, max. ∅ ∅ ∅ ∅ 17 x 80 - 110 mm, e.g. 15000, 15010, 15015, 15020, 15022, 15023, 15024, 15039, Monovettes, suitable for 11030, 11031, 11032, 11035, 11036, polyallomer Operating Manual SIGMA 2-5, page 11 of 55 03/06...
  • Page 12 Round carrier for 1 round bottom tube approx. 25 ml, max. ∅ ∅ ∅ ∅ 25,3/30 x 70 – 105 mm, e.g. 15025, 15026, 15029, 15030, 15032, 15033, polypropylene, suitable for 13034 Operating Manual SIGMA 2-5, page 12 of 55 03/06...
  • Page 13 Carrier, aluminium, for 2 culture tubes 15 ml (Nunc, Corning, Falcon, Greiner) e.g. 15115, suitable for 11030, 11031, 11032, 11035, 11036, max. radius 14.3 cm, min. radius 5.1 cm 4 000 2 558 Operating Manual SIGMA 2-5, page 13 of 55 03/06...
  • Page 14 120 mm, e.g. 15015, 15020, 15023, 15024, Monovettes and culture tubes 15 ml (Nunc, Corning, Falcon, Greiner) e.g. 15115, max. radius 13.9 cm, min. radius 7.8 cm, 4 000 2 486 angle 33° Operating Manual SIGMA 2-5, page 14 of 55 03/06...
  • Page 15 Polystyrene tube 15 ml, ∅ ∅ ∅ ∅ 17 x 100 mm, 15020 suitable for 11031, 11035, 12011, 12012, 12061, 12062, 13020, 13035, 14034, 17215 15021 Polypropylene stopper for 15020, 15023 Operating Manual SIGMA 2-5, page 15 of 55 03/06...
  • Page 16 Nylon bucket for 1 tube 15 ml, suitable for 12011, 12012, 12061, 12062 17130 Round sealing cap, polysulfone, clear for 13034 17151 Round sealing cap, polysulfone, clear for 13031 Further accessories available on request. Operating Manual SIGMA 2-5, page 16 of 55 03/06...
  • Page 17 We recommend to always fill up the tubes and to follow the recommendations of the manufacturer. Operating Manual SIGMA 2-5, page 17 of 55 03/06...
  • Page 18 Operating Manual SIGMA 2-5, page 18 of 55 03/06...

  • Page 19: Scope Of Supply

    Rotor Part No. Rotor No..............................................................Standards and Regulations Please refer to the enclosed EU-Statement of Conformity. Operating Manual SIGMA 2-5, page 19 of 55 03/06...

  • Page 20: Safety Instructions

    9. Attention! Defective lid relieving devices could cause the centrifuge lid to fall down (contact Service). Risk of bruising! Operating Manual SIGMA 2-5, page 20 of 55 03/06...

  • Page 21: Symbol Table

    Tirer la fiche de prise Vorsicht Quetschgefahr Caution! Risk of bruising Attention! Danger de blessure Drehrichtungspfeil Arrow direction of rotation → Flèche sens de rotation Heiße Oberfläche Hot surface Surface chaude Operating Manual SIGMA 2-5, page 21 of 55 03/06...

  • Page 22: Construction And Constructive Safety Measures

    General Outlay The new generation of SIGMA laboratory centrifuges is equipped with newest state-of-the-art electronics and is driven by brushless, silent and long-life asyn- chronous motors. The problem of carbon brush change is no longer existent and as there is no carbon dust pollution, operation in clean rooms is possible if the appropriate accessories are used.
  • Page 23 “Imbalance“ and the sequence of bars are flashing. The lid opens automatically after the rotor has come to a standstill. In both cases the loading has to be checked and balanced. Operating Manual SIGMA 2-5, page 23 of 55 03/06...

  • Page 24: Unpacking Of The Centrifuge

    Attention: The instrument is heavy! Please keep case for possible transport of centrifuge later. 3.1.1 Transport Safety Device The SIGMA 2-5 has a transport safety device which must be removed before start-up. Procedure: Remove the foamed plastic ring from the centrifuge chamber.

  • Page 25: Installation

    The operating voltage on the name plate must correspond to the local supply voltage! SIGMA laboratory centrifuges are units of safety class I, DIN VDE 0700, and in- clude a three wire power cord 2,5 m long with shockproof right angle plug.

  • Page 26: Installation Of Rotors And Accessories

    Before installation of the lid, the correct fastening of the rotor fixing screw must always be checked using a wrench. Operating Manual SIGMA 2-5, page 26 of 55 03/06...
  • Page 27 Operating Manual SIGMA 2-5, page 27 of 55 03/06...

  • Page 28: Initial Start-Up

    Put a rotor onto the shaft and fasten it by screwing the rotor tie-down screw clockwise onto the drive shaft. Please use the supplied rotor wrench and hold the rotor with one hand (refer to chapter 3.3 "Insertion of rotor and accessories"). Operating Manual SIGMA 2-5, page 28 of 55 03/06...

  • Page 29: Operating Panel

    The centrifuge is operated via the operating panel. Keys can be pressed when their LED is on. When power is applied, all LEDs and the display are illuminated for a short time. Operating Manual SIGMA 2-5, page 29 of 55 03/06...
  • Page 30 A flashing bar appears in the time and/or speed panel. Cancellation of this function occurs in the same way. The centrifuge can be started when • the lid is closed, • the Start-key is illuminated. Operating Manual SIGMA 2-5, page 30 of 55 03/06...
  • Page 31 A flashing Lid-key indicates that only on lid lock has locked. Press down left and right side of the lid to lock both lid locks. The lid opens automatically after standstill of the rotor! Operating Manual SIGMA 2-5, page 31 of 55 03/06...
  • Page 32 The left area is the time display, the right one the speed/RCF display. 4.2.1 Speed/RCF (Relative Centrifugal Force) Rotor dimensions and speed determine the RCF value. Entry of one value automatically determines the other one. Operating Manual SIGMA 2-5, page 32 of 55 03/06...
  • Page 33 If during the run the speed is set below the actual speed, the new set speed is indicated by a flashing bar and the drive is decelerating to the new value. Operating Manual SIGMA 2-5, page 33 of 55 03/06...

  • Page 34: Relative Centrifugal Force (Rcf)

    Please refer to chapter 1.2 “Suitable accessories“ for the maximum values for the used rotor combination. For this example rotor combinations 11035 and 11032 have been chosen. Operating Manual SIGMA 2-5, page 34 of 55 03/06...
  • Page 35 The set time is indicated by one single bar. The centrifuge run can be early terminated by pressing the Stop-key. The set time and the remaining run time remain on the display. Operating Manual SIGMA 2-5, page 35 of 55 03/06...

  • Page 36: Short-Time Operation

    The elapsed time is displayed in minutes. The continuous run mode can be left by selecting a set value as well. 4.2.3.2 Short-Time Operation There are two options: Operating Manual SIGMA 2-5, page 36 of 55 03/06...
  • Page 37 A sequence of bars is indicating the elapsed run time. One single bar at "short run" indicates that short-time operation had been selected. A flashing bar indicates the maximum speed. After termination of the short-run the elapsed run time in seconds remains on the display. Operating Manual SIGMA 2-5, page 37 of 55 03/06...
  • Page 38 The short-run can be early terminated by pressing the Stop-key. The centrifuge decelerates to standstill. The elapsed run time in seconds remains on the display. The "short run“ can be left by selecting a set value as well. Operating Manual SIGMA 2-5, page 38 of 55 03/06...
  • Page 39 (refer to chapter 7.1.2 "Density"). 17. Grease the bolts of the rotor and the nutes of the buckets slightely with grease for load bearing bolds. Operating Manual SIGMA 2-5, page 39 of 55 03/06...
  • Page 40 Never attempt to override the lid interlock system while the rotor is spinning. 16. Such materials are prohibited which chemically interact vigorously. 17. Do not spin explosive or inflammable materials. Operating Manual SIGMA 2-5, page 40 of 55 03/06...
  • Page 41 A rotor must always be loaded completely, empty places are not allowed! Opposite buckets or carriers may nevertheless be empty. Mixed loading is allowed, if opposite places are loaded with same buckets and carriers of same weight. Operating Manual SIGMA 2-5, page 41 of 55 03/06...
  • Page 42 In order to avoid corrosion, rotor incl. tie-down screw and cover seal and adapters must be cleaned and greased regularly with the supplied slushing oil (Sigma part no.: 70104 for 20 ml slushing oil). Before using detergents or decontamination agents which had not been recommended by us, the user has to contact us to make sure that such procedure would not damage the centrifuge.

  • Page 43: Service Notes

    Rotor Trunnion Pins The trunnion pins of the rotor should always be greased as only this ensures evenly swinging of buckets and thus quiet run of the centrifuge (part no. 70284 Grease). Operating Manual SIGMA 2-5, page 43 of 55 03/06...

  • Page 44: Glass Breakage

    Principally we want to point out that for centrifuging of e.g. infectious material certified and hermetically sealed accessories have to be used in order to avoid that the centrifuge is contaminated. Operating Manual SIGMA 2-5, page 44 of 55 03/06...
  • Page 45 Rubber cushions 115-118 Round carriers for 13104/ 115-118 13117, Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Round carriers for 13350/ 115-118 13550, Polypropylene Rectangular carriers, 115-118 Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Operating Manual SIGMA 2-5, page 45 of 55 03/06...
  • Page 46 50.000 cycles. Any changes like e.g. corrosion, cracks, material abrasion etc. require special attention. 5. Screw connections have to be tight. Furthermore, the earth wire must be checked regularly. Operating Manual SIGMA 2-5, page 46 of 55 03/06...
  • Page 47 If the density of the liquid is higher than 1.2 g/cm , the allowed maximum speed of the centrifuge is calculated according to the following formula: n = n , 1 ( Rho = density in g/cm Operating Manual SIGMA 2-5, page 47 of 55 03/06...

  • Page 48: Error Correction

    − When first trying to open the lid the locks are not released. The Lid-key LED is flashing. Open and close lid again. − Check/clean the lid seal. Apply talcum powder to the seal to avoid renewed sticking. Operating Manual SIGMA 2-5, page 48 of 55 03/06...
  • Page 49 • Power off/on 2, 3, 5 - 11 Internal fault Should it not be possible to repair the failure, please contact Service! Speed-RCF-Diagram An additional help is the enclosed Speed-RCF-Diagram. Operating Manual SIGMA 2-5, page 49 of 55 03/06...

  • Page 50: Declaration Of Decontamination / Return Declaration

    Fill in the forms and attach them when returning centrifuges, accessories and spare parts. Please understand that we cannot carry out any work before we have the declarations. (We recommend to make several copies of this page.) Operating Manual SIGMA 2-5, page 50 of 55 03/06...
  • Page 51 Unit / component contaminated : Unit / component unused (new) : !!! Attention – This form must be glued on outside of the box !!! ---------------------------------------------------------------------------------------------------------------------- Please make some copies before removing this page. Operating Manual SIGMA 2-5, page 51 of 55 03/06...
  • Page 53 I / we hereby declare that the information on this declaration are correct and complete. Company / Institute : Street : Postcode, City : Tel. : FAX : Name : Date : Stamp : Signature : ---------------------------------------------------------------------------------------------------------------------- Please make some copies before removing this page. Operating Manual SIGMA 2-5, page 53 of 55 03/06...
  • Page 55 Operating Manual SIGMA 2-5, page 55 of 55 03/06...

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