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Sigma 3-16K Operating Manual

Refrigerated centrifuge
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Refrigerated Centrifuge
3-16K
Operating Manual

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  • Page 1 ——————————————— Refrigerated Centrifuge 3-16K Operating Manual...
  • Page 3 We are wishing you a successful use of the centrifuge. SIGMA Laborzentrifugen GmbH P.O. Box 1713 - 37507 Osterode/Germany Phone 05522/5007-0 - Fax 05522/500712 Internet: www.sigma-zentrifugen.de eMail: info@sigma-zentrifugen.de SIGMA Service-Tel. 05522/5007-25 Operating Manual SIGMA 3-16K, page 3 of 69 03/06...
  • Page 5 Normes : EN 61326 Sigma Laborzentrifugen An der Unteren Söse 50 D-37520 Osterode 19.08.2002 Geschäftsführer Managing Director Directeur Gérant ................Fabr. Nr. Serial No. Numéro de fabrique Konformitätserklärung dreisprachig 3-16K 20020819.DOC Operating Manual SIGMA 3-16K, page 5 of 69 03/06...

  • Page 7: General Information

    3.4.3 Installation of a rotor Operating Elements Operating panel 4.1.1 Start-key / Saving a program 4.1.2 Stop-key with preselection of the soft stop and/or soft start function 4.1.3 Lid-key 4.1.4 Knobs Operating Manual SIGMA 3-16K, page 7 of 69 03/06...

  • Page 8: Care And Maintenance

    Centrifuge decelerates during operation 7.2.3 Lid cannot be opened/closed 7.2.4 Emergency lid release 7.2.5 Problems with the centrifuge? Error mode 7.3.1 Error codes Diagram speed/gravitational field Declaration of decontamination/Return declaration Leaflet Operating Manual SIGMA 3-16K, page 8 of 69 03/06...

  • Page 9: Technical Data

    %. The minimum temperatures during run are < +4 ° C a nd depend on type of rotor, speed and ambient temperature. (Allowable ambient temperature +10 ° C - +35 ° C; max . humidity 80 %.) Subject technical alterations. Operating Manual SIGMA 3-16K, page 9 of 69 03/06...
  • Page 11 15060, polyallomer 18007 Rectangular carrier for 20 glass tubes 7 ml, max. ∅ ∅ ∅ ∅ 12.3 x 80 - 105 mm, flat and round bottom tubes, e.g. 15007, 15027, polyallomer Operating Manual SIGMA 3-16K, page 11 of 69 03/06...
  • Page 12 110 mm, e.g. 15029, 15030, 15032, polypropylene 18050 Rectangular carrier for 2 tubes 50 ml, max. ∅ ∅ ∅ ∅ 35/38 x 85 - 110 mm, e.g. 15049, 15050, 15056, polyallomer Operating Manual SIGMA 3-16K, page 12 of 69 03/06...
  • Page 13 125 ml, max. ∅ ∅ ∅ ∅ 51 x 90 - 115 mm, e.g. 15125, polypropylene 18200 Rectangular carrier for 1 bottle with screw cap 200 ml, max. ∅ ∅ ∅ ∅ 57 x 90 - 115 mm, e.g. 15202, 15203, polypropylene Operating Manual SIGMA 3-16K, page 13 of 69 03/06...
  • Page 14 10 - 12 ml, max. ∅ ∅ ∅ ∅ 16.2/19 x 65 - 90 mm, e.g. 15000, 15010, 15039, polypropylene 17356 Round carrier for 16 Vacutainer/hemolyse tubes/RIA-tubes 5 - 6 ml, max. ∅ ∅ ∅ ∅ 13.5/17.5 x 70 - 90 mm, e.g. 15060, polypropylene Operating Manual SIGMA 3-16K, page 14 of 69 03/06...
  • Page 15 125 ml, max. ∅ ∅ ∅ ∅ 51 x 90 - 115 mm, e.g. 15125, polypropylene 17400 Round carrier for 1 bottle with screw cap 200 ml, max. ∅ ∅ ∅ ∅ 57 x 90 - 115 mm, e.g. 15202, 15203, polypropylene Operating Manual SIGMA 3-16K, page 15 of 69 03/06...
  • Page 16 90 - 110 mm long, polyallomer Round carrier for 12 tubes 5 ml, max. ∅ ∅ ∅ ∅ 17006 12.5 x 55 - 75 mm, e.g. hemolyse tubes or RIA-tubes 15060, polyallomer Operating Manual SIGMA 3-16K, page 16 of 69 03/06...
  • Page 17 27 - 30 ml, max. ∅ ∅ ∅ ∅ 25.4/27.5 x 85 - 100 mm, e.g. 15029, 15030, 15032, polypropylene 17049 Round carrier for 1 culture tube 50 ml 15151, polypropylene Operating Manual SIGMA 3-16K, page 17 of 69 03/06...
  • Page 18 Attention: The vessels could be damaged at speeds exceeding 13 000 rpm 14 500 16 454 14000 Casset for 20 reaction vials 0.25 - 0.4 ml 15014, suitable for 11136, polyallomer Operating Manual SIGMA 3-16K, page 18 of 69 03/06...
  • Page 19 Angle rotor 20 x 10 - 12 ml for sealed tubes 15000, 15010, 15039, incl. hermetic aluminium lid, max. radius 9.8 cm, min. radius 5.9 cm, angle 25° 10 000 10 956 Operating Manual SIGMA 3-16K, page 19 of 69 03/06...
  • Page 20 Angle rotor 6 x 78 - 85 ml for sealed tubes 13085 plus 17185, 15074, 15075, 15076, 15080, incl. hermetic aluminium lid, max. radius 9.8 cm, min. radius 2.8 cm, angle 25° 8 000 7 012 Operating Manual SIGMA 3-16K, page 20 of 69 03/06...
  • Page 21 Reaction vials 0.5 ml, ∅ ∅ ∅ ∅ 7.9/10 x 28/31 mm, 15005 1 pack contains 100 pcs., suitable for 13002, 17350 15008 Reaction vials 1.5 ml, 1 pack contains 100 pcs., suitable for 12110, 12131, 12154, 12348, 14002, 17008, 17353, 18002 Operating Manual SIGMA 3-16K, page 21 of 69 03/06...
  • Page 22 13060, 13081, 17019, 17345, 18016, 19777 Teflon tube with screw cap 28 ml, ∅ ∅ ∅ ∅ 25.3 x 15029 96 mm, suitable for 12158, 13083, 17030, 17370, 18030 Operating Manual SIGMA 3-16K, page 22 of 69 03/06...
  • Page 23 Stainless steel tube 85 ml, sealable with sealing cap 17185, ∅ ∅ ∅ ∅ 38/40 x 100/107 mm, suitable for 12155, 12159, 17085, 17385, 18085 17185 Stainless steel sealing cap for 13085 Operating Manual SIGMA 3-16K, page 23 of 69 03/06...
  • Page 24 17256, Ø 61.4 x 125 mm, suitable for 17347 17256 Stainless steel sealing cap for 13255 15247 Teflon bottle with screw cap 250 ml, Ø 61.4 x 122 mm, suitable for 17347 Operating Manual SIGMA 3-16K, page 24 of 69 03/06...
  • Page 25 Glass tube 50 ml, ∅ ∅ ∅ ∅ 34 x 100 ml, suitable for 15050 17050, 17375, 18050 15056 ditto, graduated Glass tube 100 ml, ∅ ∅ ∅ ∅ 44 x 100 mm, suitable 15100 for 17100, 17101, 17390, 18100 15106 ditto, graduated Operating Manual SIGMA 3-16K, page 25 of 69 03/06...
  • Page 26 2 doors and space for accessories, movable on lockable castors, dimensions: w 490, d 635, h 490 mm 17913 Fasteners for table 17919 Further accessories are available on request. Operating Manual SIGMA 3-16K, page 26 of 69 03/06...

  • Page 27: Maximum Speed For Tubes

    We recommend to always fill up the tubes and to follow the recommendations of the manufacturer. Operating Manual SIGMA 3-16K, page 27 of 69 03/06...
  • Page 28 Operating Manual SIGMA 3-16K, page 28 of 69 03/06...

  • Page 29: Scope Of Supply

    Rotor Part No. Rotor No..............................................................Standards and Regulations Please refer to the enclosed EU-Statement of Conformity. Operating Manual SIGMA 3-16K, page 29 of 69 03/06...

  • Page 30: Safety Instructions

    Attention! Defective lid relieving devices could cause the centrifuge lid to fall down (contact Service). Risk of bruising! Operating Manual SIGMA 3-16K, page 30 of 69 03/06...

  • Page 31: Symbol Table

    Tirer la fiche de prise Vorsicht Quetschgefahr Caution! Risk of bruising Attention! Danger de blessure Drehrichtungspfeil Arrow direction of rotation → Flèche sens de rotation Heiße Oberfläche Hot surface Surface chaude Operating Manual SIGMA 3-16K, page 31 of 69 03/06...

  • Page 32: Construction And Constructive Safety Measures

    General Outlay The new generation of SIGMA laboratory centrifuges is equipped with newest state-of-the-art electronics and is driven by brushless, silent and long-life asyn- chronous motors. The problem of carbon brush change is no longer existent and as there is no carbon dust pollution, operation in clean rooms is possible if the appropriate accessories are used.
  • Page 33 “Imbalance“ is flashing and “ERROR“ is displayed. The centrifuge has to be opened with the lid key. In both cases the loading has to be checked and balanced. Operating Manual SIGMA 3-16K, page 33 of 69 03/06...

  • Page 34: Unpacking Of The Centrifuge

    Please keep case for possible transport of centrifuge later. 3.1.1 Transport Safety Device (made of foamed material) The SIGMA 3-16K has a transport safety device between the rotor chamber and the motor. It is accessible after opening of the centrifuge lid (refer to chapter 3.3 up to 3.4.2 or 7.2.4“...

  • Page 35: Installation

    The operating voltage on the name plate must correspond to the local supply voltage! SIGMA laboratory centrifuges are units of safety class I, DIN VDE 0700, and in- clude a three wire power cord 2,5 m long with shockproof right angle plug. The instrument has thermal fuses.

  • Page 36: Installation Of Rotors And Accessories

    Should the centrifuge be operated with very uneven load, the imbalance monitoring will switch off the drive. An imbalance warning would be displayed (refer to 4.1). Operating Manual SIGMA 3-16K, page 36 of 69 03/06...
  • Page 37 To increase life of rotors and seals the rotors must be cleaned with slushing oil and the seals and thread areas with vaseline or grease after cleaning. Attention! Please follow the special comments of chapter 1.5. Operating Manual SIGMA 3-16K, page 37 of 69 03/06...
  • Page 38 Operating Manual SIGMA 3-16K, page 38 of 69 03/06...

  • Page 39: Initial Start-Up

    Put a rotor onto the shaft and fasten it by screwing the rotor tie-down screw clockwise onto the drive shaft. Please use the supplied rotor wrench (refer to chapter 3.3 "Installation of rotors and accessories"). Operating Manual SIGMA 3-16K, page 39 of 69 03/06...

  • Page 40: Operating Panel

    The centrifuge is operated via the operating panel. Keys can be pressed when their LED is on. When power is applied, all LEDs and the display are illuminated for a short time. Operating Manual SIGMA 3-16K, page 40 of 69 03/06...
  • Page 41 Activation or deactivation of “soft stop“ and/or “soft start“ by pressing the stop-key. the centrifuge is running, stop-key illuminated: Press the stop-key. “soft stop“ and/or “soft start“ can be activated or deactivated during deceleration by pressing the stop-key again. Operating Manual SIGMA 3-16K, page 41 of 69 03/06...
  • Page 42 Remark: “set“ is automatically deactivated after 60 seconds, if no further changes are made, the lock symbol appears. Thereafter, changes are impossible. Right knob: Preselection of the desired set value after activation of “set“. Operating Manual SIGMA 3-16K, page 42 of 69 03/06...
  • Page 43 Symbol lock Temperature Speed Rotor, Imbalance, run mode 4.2.1 Speed/RCF (Relative Centrifugal Force) Rotor dimensions and speed determine the RCF value. Entry of one value automati- cally determines the other one. Operating Manual SIGMA 3-16K, page 43 of 69 03/06...

  • Page 44: Relative Centrifugal Force (Rcf)

    Preselection of 1 x g by pressing and holding (2 – 3 seconds) the stop key. Please refer to chapter 1.2 “Suitable accessories“ for the maximum values for the used rotor combination. Operating Manual SIGMA 3-16K, page 44 of 69 03/06...
  • Page 45 The time value as well as different time modes can be set by turning the right knob. If the time is changed during centrifugation, the total newly entered period is run, time elapsed before is not considered. Operating Manual SIGMA 3-16K, page 45 of 69 03/06...
  • Page 46 The set time is indicated by one single bar. The centrifuge run can be early terminated by pressing the stop-key. The set time and the remaining run time remain on the display. Operating Manual SIGMA 3-16K, page 46 of 69 03/06...

  • Page 47: Continuous Operation Mode

    The elapsed time is displayed in minutes. The continuous run mode can be left by selecting a set value as well. 4.2.3.2 Short-Time Operation There are two options: Operating Manual SIGMA 3-16K, page 47 of 69 03/06...
  • Page 48 A sequence of bars is indicating the elapsed run time. One single bar at "short run" indicates that short-time operation had been selected. After termination of the short-run the elapsed run time in seconds remains on the display. Operating Manual SIGMA 3-16K, page 48 of 69 03/06...
  • Page 49 The short-run can be early terminated by pressing the stop-key. The centrifuge decelerates to standstill. The elapsed run time in seconds remains on the display. The "short run“ can be left by selecting a set value as well. Operating Manual SIGMA 3-16K, page 49 of 69 03/06...
  • Page 50 This would reduce life of the compressor. We recommend precooling at 5000 rpm. The obtainable temperatures depend on rotor type, speed and ambient temperature. (A change of the limit values might cause deviations.) Operating Manual SIGMA 3-16K, page 50 of 69 03/06...
  • Page 51 If the used rotor is not the same than the preselected one, the used rotor is displayed and the preselected speed is corrected. Preselected rotor 12158 Changed to rotor 12150 Operating Manual SIGMA 3-16K, page 51 of 69 03/06...
  • Page 52 The vessels should be placed symmetri- cally so that the rotor is loaded evenly. Loading e.g. only one position is not allowed. 9. Load all positions of swing-out rotors. 10. Load vessels outside the centrifuge. Operating Manual SIGMA 3-16K, page 52 of 69 03/06...
  • Page 53 17. When centrifuging substances with a density > 1,2 g/cm the allowable max. speed must be reduced (refer to chapter 7.1.2 "Density"). 18. Grease joints of buckets and rotor pins in swing-out rotors. Operating Manual SIGMA 3-16K, page 53 of 69 03/06...
  • Page 54 14. Lifting or moving of the centrifuge during operation. Leaning against or resting on the centrifuge is not allowed. 15. Do not place potential dangerous material - e.g. glass vessels containing liquids - near the centrifuge. Operating Manual SIGMA 3-16K, page 54 of 69 03/06...
  • Page 55 19. Substances which could damage the material of the centrifuge, the rotors or the adapters must not be centrifuged. Infectious, toxic, pathogene or radioactive substances must be centrifuged in certified rotors and vessels only and all necessary safety precautions are taken. Operating Manual SIGMA 3-16K, page 55 of 69 03/06...
  • Page 56 In order to avoid corrosion, rotor incl. tie-down screw and cover seal and adapters must be cleaned and greased regularly with the supplied slushing oil (Sigma part no.: 70104 for 20 ml slushing oil). Before using detergents or decontamination agents which had not been recommended by us, the user has to contact us to make sure that such procedure would not damage the centrifuge.

  • Page 57: Glass Breakage

    The condenser should therefore be regularly checked for dirt and cleaned if necessary. Generally blowing of the lamella with compressed air from inside and outside is sufficient (or a simple hoovering with a vacuum cleaner). Operating Manual SIGMA 3-16K, page 57 of 69 03/06...
  • Page 58 Principally we want to point out that for centrifuging of e.g. infectious material certified and hermetically sealed accessories have to be used in order to avoid that the centrifuge is contaminated. Operating Manual SIGMA 3-16K, page 58 of 69 03/06...
  • Page 59 Rubber cushions 115-118 Round carriers for 13104/ 115-118 13117, Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Round carriers for 13350/ 115-118 13550, Polypropylene Rectangular carriers, 115-118 Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Operating Manual SIGMA 3-16K, page 59 of 69 03/06...
  • Page 60 50.000 cycles. Any changes like e.g. corrosion, cracks, material abrasion etc. require special attention. 5. Screw connections have to be tight. Furthermore, the earth wire must be checked regularly. Operating Manual SIGMA 3-16K, page 60 of 69 03/06...
  • Page 61 If the density of the liquid is higher than 1.2 g/cm , the allowed maximum speed of the centrifuge is reduced according to the following formula: n = n , 1 ( Rho = density in g/cm Operating Manual SIGMA 3-16K, page 61 of 69 03/06...

  • Page 62: Error Correction

    (please refer to chapter 7.3.1 "Error codes"). 7.2.3 Lid cannot be Opened/Closed − When first trying to open the lid the lock is not released. The Lid-key LED is flashing. Open and close lid again. Operating Manual SIGMA 3-16K, page 62 of 69 03/06...
  • Page 63 Temperature sensor Excess temperature (>50° C) • Let centrifuge cool down Should it not be possible to repair the failure, please contact Service! Speed-RCF-Diagram An additional help is the enclosed Speed-RCF-Diagram. Operating Manual SIGMA 3-16K, page 63 of 69 03/06...

  • Page 64: Declaration Of Decontamination / Return Declaration

    Fill in the forms and attach them when returning centrifuges, accessories and spare parts. Please understand that we cannot carry out any work before we have the declarations. (We recommend to make several copies of this page.) Operating Manual SIGMA 3-16K, page 64 of 69 03/06...
  • Page 65 Unit / component contaminated : Unit / component unused (new) : !!! Attention – This form must be glued on outside of the box !!! ---------------------------------------------------------------------------------------------------------------------- Please make some copies before removing this page. Operating Manual SIGMA 3-16K, page 65 of 69 03/06...
  • Page 67 I / we hereby declare that the information on this declaration are correct and complete. Company / Institute : Street : Postcode, City : Tel. : FAX : Name : Date : Stamp : Signature : ---------------------------------------------------------------------------------------------------------------------- Please make some copies before removing this page. Operating Manual SIGMA 3-16K, page 67 of 69 03/06...
  • Page 69 Operating Manual SIGMA 3-16K, page 69 of 69 03/06...

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