Thermo Scientific Dionex ICS Series Operator's Manual page 62

Dionex PDA Operator's Manual
Negative Approximate Negative
Absorbance Absorbance at 210 nm
Level
0 –2200 mAU
1 (default) –2300 mAU
2 –2600 mAU
3 –2600 mAU
4 –2700 mAU*
5 –2700 mAU*
6 –2700 mAU*
* These signals are saturated and will not report a further negative value.
1. Open the Chromeleon 7 Command window (see
6.8 Commands dialog box (see
2. Select NegativeAbsorptionLevel, enter a new setting, and execute the
command.
3. Select Autozero and execute the command to run the autozero routine.
4. Select the LampIntensity command to check the intensity of the deuterium
lamp. The reading should be above 10 million counts for a standard or semi-
preparative cell or above 3 million counts for a semi-micro cell.
If the reading is lower than it should be, select UV_Calibration Intensity and
execute the command to calibrate the intensity of the deuterium lamp.
Afterward, check the lamp intensity again. If the reading is still too low, clean
the flow cell (see
5. You may continue increasing the negative absorbance level if the range is
required; however, be aware that baseline noise may increase as the negative
absorbance increases.
54
Approximate Negative
Absorbance at 240 nm
–50 mAU
–100 mAU
–200 mAU
–300 mAU
–400 mAU
–500 mAU
–600 mAU
page 32), and select UV.
Section 5.2) or replace the lamp (see
Approximate Negative
Absorbance at 254 nm
–300 mAU
–350 mAU
–450 mAU
–550 mAU
–630 mAU
–660 mAU
–760 mAU
page 31) or the Chromeleon
Section 5.7).
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