3.10.4 Tips For Dark-Field Microscopy - Nikon ECLIPSE Si Instructions Manual

3.10.4 Tips for Dark-field Microscopy

Notes on limits on objectives
Objectives that can be used for dark-field microscopy are limited to the following:
• For dark-field microscopy:
• For observation using a bright-field position: NA 1.25 or lower
Note on the bright-field position of the dark-field slider
Objectives that can be used in the bright-field position of the dark-field slider have different conditions than
for regular bright-field microscopy.
With objectives of NA 1.0 or higher, vignetting might occur in the periphery, causing the image to darken.
Note on eye protection during slider operation
Move your eyes away from the binoculars during position switching of dark-field sliders.
When you switch from a dark field to a bright field, bright light might reach the binoculars.
Chapter 3 Detailed Explanation
NA 0.65 or lower
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