Nikon ECLIPSE Si Instructions Manual page 72

6 Bring an objective into the optical path.
Rotate the revolving nosepiece to bring the objective
of the desired magnification into the optical path.
Do not directly switch from the 4x objective to
the 100x objective. Be sure to focus on the
specimen with the 10x objective before using
the objective of a higher magnification.
When using an oil-immersion objective
Apply immersion oil between the specimen and the
objective.
When light intensity management is on
When the light intensity management function is
turned on (LIM), bringing the objective into the
optical path loads the memorized brightness.
7 Focus on the specimen.
Rotate the coarse and then fine focus knobs to focus
on the specimen.
Adjust the brightness of diascopic illumination and
move the observation region by moving the stage as
required.
8 Adjust the field diaphragm.
Turn the field diaphragm dial to resize the field
diaphragm image so that it is circumscribed with the
field of view.
Adjustment of field diaphragm
Each time you change the objective, adjust the
field diaphragm.
9 Observe the specimen using the simple
polarizing microscopy method.
Turn the stage and focus knobs to observe the
specimen.
To observe the specimen with a different
magnification, repeat steps 6 to 8.
Chapter 2 Microscopy Techniques
70
Switch to the desired objective.
Focus on the specimen.
Adjust it so that the
image is
circumscribed with
the field of view
Adjust the field diaphragm.