Configuration For Phase Contrast Microscopy - Nikon ECLIPSE Si Instructions Manual

4.2.2

Configuration for Phase Contrast Microscopy

When performing phase contrast microscopy, assemble the microscope in the basic configuration for
bright-field microscopy, and then attach the following optical parts to the microscope:
(1) Phase contrast sliders
There are two kinds of phase contrast sliders: Slider for 10x-40x and slider for 100x). Objectives that
can be used are limited to the following:
Phase contrast slider
(2) Phase contrast objective (Ph objective)
Attach an objective with a PH code to the nosepiece. See (5) Installing the objective in "4.2.1 Basic
Configuration (for Bright-field Microscopy)."
Combination of optical parts
Confirm in advance that the slider is paired with a supported objective with a supported PH code.
(3) GIF filter (green interference filter)
When performing phase contrast microscopy, mounting a field lens improves the contrast of the phase
contrast image. See (8) Others in "4.2.1 Basic Configuration (for Bright-field Microscopy)."
(4) Centering telescope
Attach the binoculars when adjusting the annular diaphragm position. See (8) Others in "4.2.1 Basic
Configuration (for Bright-field Microscopy)."
Attaching a Phase Contrast Slider
Insert a slider for phase contrast microscopy into the slot on the side of the condenser.
1. Set the aperture diaphragm lever of the condenser
to the NA 1.25 side.
2. Pull the dummy slider out of the slot on the right
side of the condenser.
3. Remove the knob from the phase contrast slider.
The knob is in the form of a screw. Unscrew the knob
to remove it.
4. Insert the slider into the slot on the side of the
condenser.
Turn the slider so that the centering screw faces the
front of the microscope.
The slider has protrusions to prevent incorrect
insertion.
5. Attach the knob to the slider you attached.
Attach the knob to the front of the slider.
Chapter 4 Assembly
Supported objectives for phase contrast sliders
For 10x to 40x
For 100x
Objective
PH code Supported NA
PH1
PH2 0.55 to 0.85
PH3
Insert the phase contrast slider.
103
0.25 to 0.5
0.9 to 1.4