Nikon ECLIPSE Si Instructions Manual page 60

5 Bring the 10x objective into the optical path.
Rotate the revolving nosepiece to swing the 10x
objective into the optical path.
Turning the nosepiece
The objective will click into place when rotated into
position.
6 Place the specimen on the stage, and bring the
observation region into the optical path.
(1) Use the knob of the claw on the specimen holder
to open the claw.
(2) Place the specimen slide on the stage with the
coverglass facing upward, and put the claw back
to fix it.
(3) Turn the stage knobs to bring the observation
region of the specimen into the optical path.
Let illumination light go through the specimen sealed
under the cover glass.
Using the specimen holder
Two glass slides can be placed in two rows on the
specimen holder.
7 Turn on illumination and adjust the brightness.
The illumination on/off state and the brightness value
are shown on the status display.
(1) Press the illumination on/off button to turn on
illumination.
When illumination is turned on, the "on" symbol is
shown on the status display.
(2) Adjust the brightness by rotating the brightness
control knob.
The brightness is shown in a bar graph on the status
display.
When light intensity management is on
When the light intensity management function is
turned on (LIM), the brightness of the selected
objective is memorized. The memorized
brightness is automatically loaded the next time
the objective is selected.
Chapter 2 Microscopy Techniques
58
10x
Set the magnification of the objective to 10x.
Knob
Claw
Set the specimen.
Bring the observation region into the optical path.
Brightness
control knob
"On" symbol
Turn on illumination and adjust the brightness.
Specimen (Up to two
glass slides can be
placed.)
Illumination on/off
button
Brightness bar
graph