Nikon ECLIPSE Si Instructions Manual page 64

16 Perform focusing and centering of the condenser.
(1) Turn the field diaphragm dial to narrow down the
field diaphragm until the field diaphragm image
can be seen in the field of view.
(2) Turn the condenser vertical movement knob to
focus on the field diaphragm image.
Focus on the field diaphragm image so that it's
outline is clearly seen.
(3) Turn the condenser centering knob to perform
centering of the condenser.
Move the condenser so that the field diaphragm
image is in the center of the field of view.
(4) Turn the field diaphragm dial to resize the field
diaphragm image so that it is circumscribed with
the field of view.
Field diaphragm image
Viewfield
Focus on the field diaphragm image, move it to the center of the
field of view, and resize the image so that it fits the field of view.
17 Observe the specimen.
Turn the stage and focus knobs to observe the
specimen.
To observe the specimen with a different
magnification, repeat steps 13 to 17.
18 Turn off the microscope.
(1) Press the illumination on/off button to turn off
illumination.
The "on" symbol disappears from the status display.
(2) Unplug the power cord from the electrical outlet
Chapter 2 Microscopy Techniques
62
(1) Narrow down the field diaphragm until the field
diaphragm image can be seen.
(2) Focus on the field diaphragm image.
(3) Move the field diaphragm image to the center of the
field of view.
(4) Resize the field diaphragm image so that it is
circumscribed with the field of view.
Perform focusing and centering of the condenser.
Turn off illumination.
Narrow down the field
diaphragm until the
field diaphragm image
can be seen.
Illumination on/off
button