Nikon ECLIPSE Si Instructions Manual page 75

3 Bring the diascopic fluorescence objective into
the optical path.
Turn the nosepiece to bring the objective into the
optical path.
When light intensity management is on
When the light intensity management function is
turned on (LIM), bringing the objective into the
optical path loads the memorized brightness.
Note on using light intensity management
If diascopic fluorescence microscopy was
being performed last time the microscope was
used, beware that bright light might enter the
optical path.
4 Focus on the specimen.
Rotate the coarse and then fine focus knobs to focus
on the specimen.
Adjust the brightness of diascopic illumination and
move the observation region by moving the stage as
required.
5 Adjust the field diaphragm.
Turn the field diaphragm dial to resize the field
diaphragm image so that it is circumscribed with the
field of view.
Adjustment of field diaphragm
Each time you change the objective, adjust the
field diaphragm.
Chapter 2 Microscopy Techniques
73
Switch to the diascopic fluorescence objective.
Focus on the specimen.
Adjust the field diaphragm.
Adjust it so that the
image is
circumscribed with
the field of view