Nikon ECLIPSE Si Instructions Manual page 76

6 Bring the excitation filter and barrier filter into the
optical path, and adjust the illumination to set the
microscope to diascopic fluorescence
microscopy.
(1) From the right side, push the EX filter slider
inserted in the slot on the side of the condenser.
This sets the slider to the GFP position.
The excitation filter is brought into the optical path.
Move your eyes away the eyepieces during
position switching.
Fluorescence microscopy is performed with
diascopic illumination set to maximum.
Therefore, bright light might reach the
binoculars when the position of the
fluorescence microscopy slider is switched.
Move your eyes away from the binoculars
during position switching.
Push the slider into the click position. The aperture
diaphragm lever is automatically set to fully open (NA
1.25), and is no longer operable.
(2) Push the BA filter slider all the way into the front
slot of the nosepiece.
The barrier filter is brought into the optical path.
(3) Adjust the brightness of diascopic illumination.
Brighten the diascopic illumination so that the
specimen is excited and emits fluorescence.
Illumination during diascopic fluorescence
microscopy
To perform fluorescence microscopy, use
illumination close to the maximum illumination
intensity.
7 Observe the specimen using the diascopic
fluorescence microscopy method.
Turn the stage and focus knobs to observe the
specimen.
To observe the specimen with a different
magnification, repeat steps 6 to 8.
Chapter 2 Microscopy Techniques
74
(1) Set the EX filter slider to the GFP position.
(2) Push the BA filter slider in all the way.
Brightness
control knob
(3) Adjust the brightness of diascopic illumination.
Set the microscope to use diascopic fluorescence.
BA filter slider
EX filter slider