Nikon ECLIPSE Si Instructions Manual page 77

8 Switch to bright-field microscopy.
(1) Darken diascopic illumination, and then turn off
diascopic illumination.
Turn the brightness control knob to darken diascopic
illumination. Then, press the illumination on/off button
to turn off diascopic illumination.
(2) Pull the BA filter slider up to the click position.
The barrier filter is removed from the optical path.
Move your eyes away the eyepieces during
position switching.
Beware that if you remove the barrier filter
from the optical path while looking into the
eyepiece during diascopic fluorescence
microscopy, light might flash into your eye,
causing eye damage.
When setting the microscope back to
bright-field microscopy, either turn off
diascopic illumination or keep your eyes away
from the eyepiece.
(3) Insert the EX filter slider from the left side up to
the click position, and set it at the BF position (for
bright-field microscopy).
The empty position is brought into the optical path.
(4) To continue observation, turn on diascopic
illumination.
Press the illumination on/off button to turn on
diascopic illumination.
Chapter 2 Microscopy Techniques
75