Problems In Phase Contrast Microscopy - Nikon ECLIPSE Si Instructions Manual

Image not bright enough
Problems
Setting of the brightness control knob
Aperture diaphragm is closed too far.
Field diaphragm is closed too far.
Darkness at the periphery, no viewfield seen, or uneven viewfield brightness
Problems
Revolving nosepiece is not in click-stop position. (the
objective is not centered in the optical path).
Dirt or dust on the lens (condenser, objective, field
lens, eyepiece), specimen
Image dark on one side (One side of the viewfield (up, down, right, or left) is not in focus.)
Problems
Revolving nosepiece is not in click-stop position.
Specimen rises from stage surface.
The thickness of the sample (section) is not uniform
and has a tilt.
Image shifts during focus (Becomes asymmetrically defocused when moving the focal point.)
Problems
Revolving nosepiece is not in click-stop position.
Specimen rises from stage surface.
The thickness of the sample (section) is not uniform
and has a tilt.
5.1.2

Problems in Phase Contrast Microscopy

Incomplete field of view or no field of view seen
Problems
The phase contrast slider is not at the click position
(the annular diaphragm is not in the optical path).
No phase contrast image seen
Problems
You are not using a phase contrast slider.
You are not using a Ph objective.
Chapter 5 Troubleshooting
Check Items
Adjust the brightness by rotating the brightness control

knob.
Open properly.

Open properly.

Check Items
Revolve to click-stop position (swing the objective

correctly into the optical path).
Clean the lens.

Check Items
Revolve to click-stop position.

Stabilize it using the holder.

Prepare a sample (section) of uniform thickness.

Check Items
Revolve to click-stop position.

Stabilize it using the holder.

Prepare a sample (section) of uniform thickness.

Check Items
Move the slider until it clicks.

Check Items
Mount a phase contrast slider and bring the

appropriate position into the optical path.
Bring a Ph objective containing a phase plate into the

optical path.
112
Reference
3.2.1
3.8
3.9
Reference
3.4.1
6.1
3.3.1
Reference
3.4.1
―
―
Reference
3.4.1
―
―
Reference
2.2
4.2.2
Reference
2.2
4.2.2
2.2
3.11
4.2.2