Nikon ECLIPSE Si Instructions Manual page 67

3 Bring the phase contrast objective into the
optical path.
Turn the nosepiece to bring the phase contrast
objective (Ph objective) of the desired magnification
into the optical path.
The phase contrast ring inside the objective is
brought into the optical path.
Check the objective magnification and the PH code.
Do not directly switch from the 4x objective to
the 100x objective. Be sure to focus on the
specimen with the 10x objective before using
the objective of a higher magnification.
When using an oil-immersion objective
Apply immersion oil between the specimen and the
objective.
When light intensity management is on
When the light intensity management function is
turned on (LIM), bringing the objective into the
optical path loads the memorized brightness.
4 Focus on the specimen.
Rotate the coarse and then fine focus knobs to focus
on the specimen.
5 Insert the phase contrast slider and bring the
phase contrast position (PH) into the optical path.
(1) Set the aperture diaphragm lever at the fully open
position.
When inserting or removing sliders, always make
sure the aperture diaphragm lever is at the fully open
position.
(2) Pull the dummy slider out of the slot on the side
of the condenser.
The 100x phase contrast slider has an empty
position. Bright-field microscopy can be performed by
attaching the slider and bringing the empty position
into the optical path in advance.
(3) Insert the phase contrast slider so that the
centering screw of the annular diaphragm faces
the front of the microscope, and the operating
knob is on the left side.
Remove the slider knob, insert the slider facing the
appropriate direction, and re-attach the knob to the
slider.
Bring the position of the same PH code as the
objective into the optical path. The annular
diaphragm is brought into the optical path.
Chapter 2 Microscopy Techniques
65
Switch to the phase contrast objective.
Focus on the specimen.
Inserting the phase contrast slider
(1) Remove the knob from the slider.
The knob is in the form of a screw. Unscrew
the knob to remove it.
(2) Insert the slider into the slot on the side of
the condenser.
The slider has protrusions to prevent
incorrect insertion.
(3) Attach the knob to the slider you attached.
Attach the slider knob from the left side of the
condenser.
Insert the phase contrast slider.