Nikon ECLIPSE Si Instructions Manual page 79

4 Fully open the filed diaphragm and the aperture
diaphragm.
(1) Turn the field diaphragm dial clockwise until it
reaches the limit.
(2) Set the aperture diaphragm lever of the
condenser at the fully open position (leftmost).
Condenser position
Move the condenser to the position where the
image is clear under dark-field illumination.
The position slightly lower than the upper limit
would be suitable.
5 Bring an objective into the optical path.
Rotate the revolving nosepiece to bring the objective
of the desired magnification into the optical path.
Do not directly switch from the 4x objective to
the 100x objective. Be sure to focus on the
specimen with the 10x objective before using
the objective of a higher magnification.
When using an oil-immersion objective
Apply immersion oil between the specimen and the
objective.
When light light intensity management is on
When the light intensity management function is
turned on (LIM), bringing the objective into the
optical path loads the memorized brightness.
6 Adjust the brightness of diascopic illumination.
Adjust the brightness of diascopic illumination by
turning the brightness control knob.
In dark-field microscopy, the background darkens,
and the target is illuminated from an angle. Adjust the
brightness to observe the specimen as desired.
7 Focus on the specimen.
Rotate the coarse and then fine focus knobs to focus
on the specimen.
8 Observe the specimen using the dark-field
microscopy method.
Turn the stage and focus knobs to observe the
specimen.
To observe the specimen with a different
magnification, repeat steps 5 to 7.
Chapter 2 Microscopy Techniques
77
Fully open the field diaphragm and
the aperture diaphragm.
Switch to the suitable objective.
Brightness
control knob
Adjust the brightness of diascopic illumination.
Focus on the specimen.
At the fully open
position
Turn it clockwise
until it reaches
the limit.