Configuration For Diascopic Fluorescence Microscopy - Nikon ECLIPSE Si Instructions Manual

4.2.4

Configuration for Diascopic Fluorescence Microscopy

When performing diascopic fluorescence microscopy, assemble the microscope in the basic configuration for
bright-field microscopy, and then attach the following optical parts to the microscope.
(1) E2-F-FL Dia-FL Set GFP-B
The E2-F-FL Dia-FL Set GFP-B is composed of the following two parts:
• EX filter slider
The EX filter slider is installed in the slot on the side of the condenser. This slider is for switching the
empty position for bright-field microscopy and the excitation filter for diascopic fluorescence.
• BA filter slider
The BA filter slider is installed in the slot on the front of the nosepiece. When you push this slider in
all the way, the barrier filter is brought into the optical path. When you pull this slider out until the
click position, the escape position for bright-field microscopy is brought into the optical path.
• Shielding plate
Attach the shielding plate to the tip of the arm using two fixing screws. This will prevent strong light
including UV light from specimen through the condenser from flashing into your eyes.
(2) Objective supporting diascopic fluorescence microscopy
The following three types of objectives can be used for diascopic fluorescence microscopy:
• CFI E Plan 4x
• CFI E Plan 10x
• CFI E Plan 40x
Restriction on using objectives
60x and 100x objectives cannot be used for diascopic fluorescence microscopy.
Chapter 4 Assembly
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