B
Appendix B Troubleshooting
Initialization fails
Observation
Reagent Check fails
Bottle leak check fails
44
Possible cause
A wash failure was detected.
• Bottle seal was not tight.
• Bottle was damaged or
defective.
Ion GeneStudio S5 Instrument User Guide
Recommended action
1. Perform a manual
cleaning of the
sequencer, see page 16.
2. Replace the Reagents
cartridge, Wash Solution
bottle, and Cleaning
Solution bottle, then
repeat initialization of
the sequencer.
3. If the initialization
completes without
failure, press Home,
then continue with your
sequencing run.
4. If the Reagent Check
continues to fail, contact
Technical Support.
Ensure that you install the Ion
S5
Wash Solution bottle with
™
the instrument door fully
open. If you install the bottle
impeded by a partially closed
door, the port can be
obstructed, and a faulty
installation and wash failure
can result.
1. Finger-tighten the
bottles.
2. If the bottle continues to
leak, replace the bottle.
3. If leak check continues
to fail, contact Technical
Support.