Test The Minifuge - Thermo Scientific Ion GeneStudio S5 User Manual

C
Appendix C Supplemental procedures
Set up and test the Ion Chip

Test the minifuge

50
Minifuge
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4. Install the two buckets. Position the buckets with the larger semi-circular cut-outs
facing out, and ensure that the buckets hang freely.
1
Correct orientation
1
Incorrect orientation
2
1. Prepare two previously-used chips:
a. Inject 100 µL of isopropanol two times into the loading port of each chip.
After each injection, remove the expelled liquid from the opposite port.
Note: Use 50 µL volume of isopropanol if testing Ion PGM
chips.
b. Aspirate the remaining isopropanol from the flow cells for 5–10 seconds.
Confirm that the chips are dry.
Note: To aspirate the isopropanol, attach a P200 pipette tip to a vacuum
line, then place the pipette tip in the chip loading port.
2
sequencing
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Ion GeneStudio S5 Instrument User Guide