Solid-State Buffer Leak; Pulse Clean The Sequencer - Thermo Scientific Ion GeneStudio S5 User Manual

B
Appendix B Troubleshooting

Solid-state buffer leak

Solid-state buffer leak
Observation
Two or more of the following
observed
• Excessive read trimming
or the number of short
reads is significantly
higher than expected.
• Percentage of low quality
reads is significantly
higher than expected.
• Poor sequencing
performance or no Test
Fragments.
Pulse clean the
sequencer
46
Possible cause
A solid-state buffer leak
occurred.
You should only pulse clean your Ion GeneStudio S5 Sequencer if directed by
Technical Support, or your Field Service Engineer (FSE), to recover instrument
performance from a diagnosed solid-state buffer leak.
Note: You must contact Technical Support, or your FSE, to diagnose whether a solid-
state buffer leak has occurred and obtain the required materials to perform the pulse
cleaning.
1. Ensure that the sequencer has completed a normal post-run cleaning. In the main
menu:
Display text
Run Instrument requires cleaning before a pulse cleaning can be
performed, see "Perform the sequencer cleaning manually" on
page 16.
Clean Instrument requires cleaning before a pulse cleaning can be
performed, see "Perform the sequencer cleaning manually" on
page 16.
Initialize Instrument is ready to be pulse cleaned. Proceed to step 2.
2. Press
(Settings)4System Tools4Pulse clean.
Recommended action
Contact Technical Support.
Perform the pulse cleaning protocol if directed
to do so by Technical Support or your Field
Service Engineer (FSE).
Description
Ion GeneStudio S5 Instrument User Guide