Thermo Scientific Ion GeneStudio S5 User Manual page 51

Ion GeneStudio S5 Instrument User Guide
2. Place the two chips prepared in step 1 in the centrifuge buckets, with the chip
notch pointing out. Add 55 µL of nuclease-free water to each chip loading well
(do not inject into the chip loading port).
Note: Use 35 µL volume of nuclease-free water if testing Ion PGM
chips.
1
Chip notch
1
3. Centrifuge for 5–10 seconds, then examine each chip.
The flow cell in each chip should be completely filled with liquid with no air
bubbles. A small volume of liquid will remain in the loading well; this is normal.
Result
The chips are NOT completely filled...
The chips ARE completely filled...
The chips have air bubbles after the
additional 10 minute centrifugation...
The chips remain completely filled...
Appendix C Supplemental procedures
Set up and test the Ion Chip
1
Action
Contact Technical Support.
Centrifuge the chips for an additional
10 minutes, then check the chips again for
air bubbles, especially near the inlet and
outlet ports.
Contact Technical Support.
The centrifuge is ready to use for chip
loading.
C
Minifuge
™
sequencing
™
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