Thermo Scientific Multidrop Combi nL User Manual page 62

Routine Operation
Instrument options
62
Thermo Scientific Multidrop Combi nL User Manual
Press .
OK
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Take the first strip (column 1) of a 96 well 1x8 strip plate (Thermo
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Scientific Microtiter 96 1x8 Strip Plate, Cat. no. 95029350, or
equivalent) and use an analytical balance (four digits) to set the tare
weight to zero.
Note You can also measure the mass of the empty strip and calculate
the difference after weighing the dispensed liquid to obtain the net
value. However, the easiest way is to set the tare weight of the empty
strip to zero so that the net mass of the dispensed liquid can be obtained
from the balance without any calculations.
Place the plate onto the plate carrier of the Multidrop Combi nL.
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Press on the keyboard to start dispensing (or
START
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at the liquid, speed and volume set in Main > Volume and Liquid.
The liquid is dispensed into the empty strip a default number of
times, depending on the set volume, to obtain a measurable
amount of mass.
The expected volume necessary to be dispensed is shown above the
line, for example, 179200 nl, in the screenshot above.
Measure the mass of the strip immediately to avoid evaporation.
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Expected volume to be dispensed
Parameters selected in the Main
menu
s
to escape)
STOP
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