Thermo Scientific Multidrop Combi nL User Manual page 49

Thermo Fisher Scientific
Use the Up and Down arrow keys to set the dispensing direction,
row wise or column wise. Note that the whole action is only effective
when 384 or 1536-well plate types are selected.
Press .
OK
The Dispensing direction function is useful in assays where reaction
times are critical.
With the 96-well plate selection, the columns with 8 wells are filled
starting from column 1 according to the selected columns, after which
the plate carrier returns to the home position and the dispensing valve
lifting mechanism to the up position. Row wise and column wise
dispensing show no difference in 96-well plate dispensing.
With the 384-well plate selection, the columns with 16 wells are filled
in two phases: first every other row (A, C, E, G, ... O) starting from the
columns selected and then the dispensing valve head carrier shifts
sideways so that the remaining rows (B, D, F, H, ... P) are filled in the
opposite order. If column wise dispensing is used, it shifts the
dispensing valve head carrier first sideways filling 16 wells and then the
plate carrier moves dispensing over the next column.
With the 1536-well plate selection, the columns with 32 wells are filled
in four phases: first every fourth row (A, E, I, M ... AC) and then the
dispensing valve head carrier shifts sideways above the rows (B, F, J, N
... AD), which are then filled in the opposite order. After three shifts
the remaining rows (D, H, L, P ... AF) are filled. Column wise
dispensing shifts the dispensing valve head carrier sideways three times
filling 32 wells and then the plate carrier moves the dispensing valve
head over the next column.
Thermo Scientific Multidrop Combi nL User Manual
Routine Operation
Protocol settings
49