Calibration; Table 4-1. Calibration Point Vs. Dispensing Volume And Dispensing Times - Thermo Scientific Multidrop Combi nL User Manual

Calibration

Thermo Fisher Scientific
The dispensing valves are suitable for high-precision and high-speed
dispensing of liquids. The valve nozzle bore geometry is designed to
achieve an optimum droplet forming when the dispensed liquid volume
is discharged from the valve. The wetted components are made from
chemically resistant materials. The wetting materials of the
microsolenoid dispensing valves, Cat. no. N07494, consist of: stainless
steel, polyetheretherketone (PEEK), sapphire, and ruby.
The functional principle of the valve is:
Not activated: Closed and Activated: Open
Depending on the loading pressure at the reagent reservoir, the
duration of the opening pulse determines the dispensing volume.
A calibration defines the valve open times for different volumes at a
certain air pressure. Calibrations are created, calculated and transferred
with the Multidrop Combi nL PC software.
Calibration is a combination of a liquid to be dispensed and a speed.
Calibration will ensure accurate dispensing with the instrument.
A calibration contains a series of five calibration points, of which each
consists of a volume/time pair. Each calibration point has a fixed valve
open time. The approximate dispensing volumes change according to
the liquid properties and dispensing speeds. Refer to the example with
water, Speed 3, in Table 4–1.
Table 4–1. Calibration point vs. dispensing volume and dispensing times
Calibration point
1
2
3
4
5
The dispensed liquids may have different properties, which affect the
valve open time needed to achieve the required volume. Thus, several
different calibrations are needed to achieve accurate dispensing results.
For more information, refer to the FILLit Software for Multidrop Combi
nL User Manual (Cat. no. N17562).
Dispensing volume
60 nl
100 nl
350 nl
3.5 µl
70 µl
Thermo Scientific Multidrop Combi nL User Manual
Routine Operation
Dispensing valves
Number of dispensings
300
150
45
18
2
27