Intended Use; Principle Of Operation; Pressure/Vacuum System; Dispensing Applications - Thermo Scientific Multidrop Combi nL User Manual

Intended use

Principle of
operation
Pressure/vacuum
Dispensing
applications
Thermo Fisher Scientific
The Multidrop Combi nL is intended for professional research use by
trained personnel. It is intended for automated nanoliter to microliter
volume dispensing of reagent into a variety of microplates and strips in
96, 384 or 1536-well plate format. Use for self-testing is excluded.
The Multidrop Combi nL (Figure 1–1) can be:
1. Used as a standalone instrument with internal software using the
keyboard and display,
2. Controlled more flexibly with a PC, with FILLit™ Software, via an
RS-232 or a USB serial port, or
3. Used as part of an automation system.
The Multidrop Combi nL has a pressure/vacuum based dispensing
system that the instrument controls. The liquid is pushed through a
system
microsolenoid dispensing valve by means of compressed air into a well.
The reagent can be dispensed into rows, columns or wells.
The pressure system can also produce vacuum, which is used for the
backflush of reagents (Empty function).
Reliable low-volume dispensing can be carried out with the Multidrop
Combi nL.
With a volume range of 50 nl to 50 µl for 96, 384 and 1536-well plates
with dispensing heights from 5 to 55 mm, the Multidrop Combi nL
offers outstanding flexibility for a wide range of applications.
Multidrop Combi nL can be used, for example, in cell and bead-based
assays and to a greater extent in drug discovery screening processes for
dispensing protein solutions (BSA), detergents, dyes and different kinds
of solutions. Compounds of interest are often solubilized in DMSO
that is ready calibrated and in store in the instrument.
The viscosity of the solutions varies considerably. Viscosity describes a
fluid's internal resistance to flow and can be thought of as a measure of
fluid friction. Thus, water is "thin", having a lower viscosity, while
glycerol is "thick", having a higher viscosity. The instrument can
calibrate the valve open times and dispense with great accuracy liquids
with different viscosities. Enzyme or antibody solutions, which often
contain glycerol, can be reliably dispensed with the Multidrop Combi
nL.
Introduction to the Multidrop Combi nL
Thermo Scientific Multidrop Combi nL User Manual
Intended use
13