Waters 2410 Operator's Manual page 82

Remote Mode
The 2410 refractometer operates in remote mode when it is under active control by a
system controller through the IEEE-488 interface. You can configure remote control of the
2410 refractometer with Waters systems such as the:
• Millennium Chromatography Manager (see
Data System Using the IEEE-488
• 600E Multisolvent Delivery System (see
PowerLine System Controller)
• 745/745B/746 Data Module (see
745/745B/746 Data Module)
• 845/860 Data Control System (see
ExpertEase System)
• 2690 Separations Module (see
Separations Module).
Changing Solvents
Caution: To avoid chemical hazards, always observe safe laboratory practices when you
are operating your system. Refer to the Material Safety Data Sheets shipped with solvents
for handling information.
When you change solvents, be aware that:
• Changes involving two miscible solvents may be made directly. Changes involving
two solvents that are not totally miscible (for example, from chloroform to water),
require an intermediate solvent (such as isopropanol).
• Temperature affects solvent miscibility. If you are running a high-temperature
application, consider the effect of the higher temperature on solvent solubility.
• Buffers dissolved in water may precipitate when mixed with organic solvents.
When you switch from a strong buffer to an organic solvent, flush the buffer out of the
system with distilled water before you add the organic solvent.
To change solvents:
1. Make sure the 2410 refractometer Purge Out line goes to waste.
2. To prevent backpressure in the column, replace the column with a union.
3. Set the pump or solvent delivery system flow rate to
4. Press 2nd Func, then Purge.
5. Let the 2410 refractometer purge for a minimum of 5 minutes.
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Section 3.2.1, Connecting to a Waters
Bus)
Section 3.2.2, Connecting to a Waters
Section 3.3.2, Connecting to the Waters
Section 3.3.4, Connecting to the Waters 845/860
Section 3.3.1, Connecting to a Stand-Alone 2690
5 mL/min.
Starting Up the 2410 Refractometer
5
81