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ionKey/MS
System Guide
715004028/Revision B
Copyright © Waters Corporation 2014
All rights reserved

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Summary of Contents for Waters ionKey/MS

  • Page 1 System Guide 715004028/Revision B Copyright © Waters Corporation 2014 All rights reserved...
  • Page 2 December 8, 2014, 715004028 Rev. B...

  • Page 3: Copyright Notice

    Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com).

  • Page 4: Customer Comments

    We seriously consider every customer comment we receive. You can reach us at tech_comm@waters.com. Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information: Contacting medium...

  • Page 5: Safety Considerations

    United States and HAR-type (or better) in Europe. The main power cord must only be replaced with one of adequate rating. For information regarding what cord to use in other countries, contact your local Waters distributor. December 8, 2014, 715004028 Rev. B...
  • Page 6 Solvent leakage hazard The source exhaust system is designed to be robust and leak-tight. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% LC eluate. Warning: • To confirm the integrity of the source exhaust system, renew the source O-rings at intervals not exceeding one year.
  • Page 7 The need to decontaminate other vacuum areas of the instrument depends on the kinds of samples the instrument analyzed and their levels of concentration. Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible.

  • Page 8: Fcc Radiation Emissions Notice

    Bottle placement prohibition To avoid injury from electric shock or fire, and to prevent Prohibited: damaging the workstation and ancillary equipment, do not place objects filled with liquid—such as solvent bottles—on these items, or expose them to dripping or splashing liquids. FCC radiation emissions notice Changes or modifications not expressly approved by the party responsible for compliance, could void the users authority to operate the equipment.

  • Page 9: Operating The Ionkey/Ms System

    Operating the ionKey/MS system ™ When operating the ionKey/MS system, follow standard quality-control (QC) procedures and the guidelines presented in this section. Applicable symbols Symbol Definition Manufacturer Date of manufacture Authorized representative of the European Community Confirms that a manufactured product complies...
  • Page 10 For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. Serial number Part number catalog number...

  • Page 11: Audience And Purpose

    Audience and purpose This guide is intended for personnel who install, operate, and maintain the ionKey/MS system source module. It presents an overview of the source’s technology and operation. Intended use of the ionKey/MS system Waters designed the ionKey/MS system to perform chromatographic separations and mass spectrometry analysis.

  • Page 12: Emc Considerations

    EMC considerations Canada spectrum management emissions notice This class A digital product apparatus complies with Canadian ICES-001. Cet appareil numérique de la classe A est conforme à la norme NMB-001. ISM classification ISM Classification: ISM Group 1 Class B This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical (ISM) instruments requirements.

  • Page 13: Ec Authorized Representative

    EC authorized representative Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX United Kingdom Telephone: +44-161-946-2400 Fax: +44-161-946-2480 Contact: Quality manager December 8, 2014, 715004028 Rev. B xiii...
  • Page 14 December 8, 2014, 715004028 Rev. B...

  • Page 15: Table Of Contents

    Safety advisories ....................viii Operating the ionKey/MS system ..............ix Applicable symbols ..................... ix Audience and purpose..................xi Intended use of the ionKey/MS system ............. xi Calibrating ......................xi Quality control ....................xi EMC considerations ................... xii Canada spectrum management emissions notice ........... xii ISM classification ....................
  • Page 16 ionKey source ..................... 23 iKey separation device ..................24 iKey separation device types ................25 2 Optimizing Performance ............... 29 General guidelines for a microscale system ..........30 Solvent recommendations ................. 31 Sample volume ....................32 Spray optimization..................... 32 Plumbing connections and retention-time delay ........33 Carryover ......................
  • Page 17 Warning symbols ....................68 Specific warnings ....................69 Notices ........................72 Warnings that apply to all Waters instruments and devices ....73 Warnings that address the replacing of fuses ..........78 Electrical and handling symbols ..............80 Electrical symbols ....................80 Handling symbols ....................
  • Page 18 xviii December 8, 2014, 715004028 Rev. B...

  • Page 19: The Ionkey/Ms System

    The ionKey/MS System ™ ® The ionKey/MS system performs the UPLC separation inside the source of the mass spectrometer. In this integrated system, the product of a chromatographic separation is introduced directly into the mass spectrometer's ionization source. Contents: Topic Page System components................

  • Page 20: System Components

    ™ • iKey separation device • Mass spectrometer ® • MassLynx mass spectrometry software The ionKey/MS system configurations can include these modules: • Binary solvent manager • Auxiliary solvent manager • Fixed-loop Sample manager • Trap-valve manager • Sample organizer •...
  • Page 21 System components ionKey/MS system: ionKey-compatible mass spectrometer ACQUITY UPLC M-Class ® (Xevo TQ-S shown) ionKey source (example shown does not include a reference probe) Solvent tray µSample iKey manager separation device µBinary solvent manager December 8, 2014, 715004028 Rev. B...

  • Page 22: For Additional Information

    1 The ionKey/MS System For additional information On the ionKey/MS System Documentation CD, you can find this additional information about the ionKey/MS system modules: • ACQUITY UPLC M-Class System Guide • ACQUITY UPLC M-Class Driver Pack Installation and Configuration Guide •...

  • Page 23: Ionkey Source

    ionKey source ionKey source The ionKey source precisely positions the iKey separation device and its integral emitter in the mass spectrometer. All fluid, electronic connections (heater and electrospray high voltage), and gas connections (sheath gas) are made inside the source. Mass spectrometers with a time-of-flight (ToF) mass analyzer use a variant of the ionKey source that includes a reference probe.

  • Page 24: Ikey Separation Device

    1 The ionKey/MS System iKey separation device To avoid damaging the iKey separation device’s fragile Notice: components, handle it with care. The iKey Separation Device Care and Use Manual, which contains See also: additional information and user recommendations. The iKey separation device eliminates plumbing connections and minimizes the potential for extra-column dispersion.

  • Page 25: Ikey Separation Device Types

    iKey separation device Alignment feature: Alignment feature Label band iKey separation device types The following iKey separation devices are available. iKey separation device Label band color Label text color function Analytical Silver Black Post-column addition Silver White Infusion Dark gray Black Diagnostic Light blue...
  • Page 26 1 The ionKey/MS System Analytical iKey separation device Analytical iKey separation devices incorporate a 150 µm 5 cm (or 10 cm) × analytical column packed with sub-2.0-µm UPLC chromatography particles. Several different analytical iKey separation devices with particles suitable for various applications are available.
  • Page 27 iKey separation device Infusion iKey separation device Infusion iKey separation devices, which are not packed with chromatography particles, are available for optimizing operation of the iKey separation device in the source. The infusion iKey separation device can also be used when you change the system solvent.
  • Page 28 1 The ionKey/MS System Flow injection iKey separation device Flow injection iKey separation devices, which have an 85-µm flow channel that is not packed with particles, are available as a fluid conduit, for direct elution. Flow injection flow path: Incoming flow from µSample...

  • Page 29: Optimizing Performance

    Optimizing Performance The guidelines that follow help ensure optimum system performance. Contents: Topic Page General guidelines for a microscale system ........30 Plumbing connections and retention-time delay......33 Carryover..................34 Contamination.................. 35 Reproducibility ................. 35 Preventing leaks ................37 Sample preparation ................. 37 December 8, 2014, 715004028 Rev.

  • Page 30: General Guidelines For A Microscale System

    2 Optimizing Performance General guidelines for a microscale system The ionKey/MS system guidelines differ from those governing standard LC practices, in part because the chromatography is performed on a microscale UPLC system. Such systems operate on a much smaller scale than standard 2.1-mm (I.D.) UPLC systems.

  • Page 31: Solvent Recommendations

    General guidelines for a microscale system Solvent recommendations To avoid increased chemical noise, or loss of sensitivity, observe Important: these solvent requirements when performing a microscale UPLC analysis that includes mass spectrometry: • Use high-quality, ultra-pure solvents, buffers, and additives (LC/MS grade, prefiltered).

  • Page 32: Sample Volume

    2 Optimizing Performance Sample volume When you perform chromatography on a micro scale, the volume of an injected sample is large compared with the volume of the column. The relatively large sample volume adversely affects samples that contain a high organic content and those that contain hydrophilic analytes.

  • Page 33: Plumbing Connections And Retention-Time Delay

    Plumbing connections and retention-time delay Plumbing connections and retention-time delay The volume of a typical iKey separation device is less than 400 nL, compared with 174 µL for a typical 2.1 × 50 mm UPLC column. By integrating microscale LC components as part of the mass spectrometer, the ionKey system avoids the leaks associated with capillary connections and emitter installation.

  • Page 34: Carryover

    2 Optimizing Performance Carryover Carryover occurs when a previously injected analyte contributes to peaks in the chromatogram of subsequent samples. In LC/MS systems, carryover occurs when a small amount of analyte remains in the system after a sample is injected. You can measure carryover by observing analyte peaks that appear when you run a blank sample immediately after an analytical sample.

  • Page 35: Contamination

    Contamination • Follow the gradient with a high-organic rinse sufficient to remove hydrophobic compounds, for example, 95% B for 5 min. Contamination To minimize contamination, ensure cleanliness during sample and solvent preparation, (for example, confirm that sample-preparation tools and bottles are cleaned thoroughly and properly).
  • Page 36 2 Optimizing Performance Compared to standard-scale separations, microscale UPLC systems Note: exhibit a significant delay between the end of a gradient profile and a return to the initial conditions (see the following figure). To ensure re-equilibration is complete, observe the system-pressure trace, to ensure the pressure returns to the initial conditions.

  • Page 37: Preventing Leaks

    Leaks can occur at any tubing connection, gasket, or seal. They occur most frequently, however, at tubing connections. To prevent leaks, follow Waters’ recommendations for the proper tightening of system fittings. Note specifically that different techniques apply to retightening fittings versus installing them for the first time.
  • Page 38 2 Optimizing Performance December 8, 2014, 715004028 Rev. B...

  • Page 39: Preparing The System

    Preparing the System You must first prime the ACQUITY UPLC M-Class system, and then prepare the ionKey source for analyzing samples. Certain ionKey/MS configurations can use a nanoACQUITY Note: UPLC. The ACQUITY UPLC M-Class System Guide. See also: Contents: Topic Page Priming the system ................

  • Page 40: Priming The System

    3 Preparing the System Priming the system For optimal system performance, prime the seal wash, the µBinary solvent manager, and the µSample manager for operation. Recommendations: • Prime the µBinary solvent manager for one minute for each solvent when the system has been idle for four or more hours, and you intend to use the solvents already in the system.

  • Page 41: Preparing The Ionkey Source

    Preparing the ionKey source Preparing the ionKey source General workflow and precautions The diagram below illustrates a general workflow when you use the ionKey/MS system: Install the infusion iKey separation device Optimize/calibrate/verify Set up reference probe (ToF instruments only) Flush/stop the infusion...
  • Page 42 3 Preparing the System To avoid damaging the iKey separation device, observe these Notice: precautions: • Handle the device with care; the component parts are fragile. • Do not subject the device to pressures that exceed 69,000 kPa (690 bar, 10,000 psi). •...

  • Page 43: Inserting The Ikey Separation Device

    The x, y, and z controls each offer a micrometer scale that helps you precisely position the sprayer tip. Visit www.waters.com to watch a video showing the insertion and See also: removal of an iKey separation device.
  • Page 44 3 Preparing the System Upon initial installation, flush the inlet line until liquid is visible in the top port. Top port To avoid puncture wounds caused by the sharp emitter Warning: tip, use care when handling the iKey separation device. Remove the iKey separation device from its protective sheath.
  • Page 45 Preparing the ionKey source Insert the iKey separation device fully in the docking port of the ionKey source. The status light begins to blink green. Result: The iKey separation device fits in the docking port only one way, Tip: with the emitter end toward the mass spectrometer and the four ports on the left-hand side.
  • Page 46 3 Preparing the System To avoid damaging the iKey separation device, if the green Notice: status light is not blinking, do not rotate the lever. Rotate the lever counterclockwise 180 degrees until the lock indicator is in the 12 o’clock position. Lock indicator in 12 o’clock position Lever in vertical position Doing so locks the iKey separation device in place and engages the...

  • Page 47: Removing The Ikey Separation Device

    Preparing the ionKey source Removing the iKey separation device To avoid damaging the iKey separation device, decompress it Notice: before you remove it from the source. To remove the iKey separation device from the ionKey source: Stop the solvent flow, and decompress the iKey separation device by one of these methods: •...

  • Page 48: Specifying Temperatures

    3 Preparing the System To avoid puncture wounds caused by the sharp, Warning: electrospray, emitter tip, use care when handling the iKey separation device. To avoid burn injuries, avoid touching the metal rings on Warning: the sides of the iKey separation device. Pull the iKey separation device from the docking port.

  • Page 49: Optimizing The Source

    Optimizing the source Optimizing the source Optimizing the ionKey source involves adjusting it for normal operation using an infusion iKey separation device to optimize the spray position, nanoflow gas pressure, and the capillary voltage. (The device does not contain chromatography particles.) The online Help for the console software, for detailed instructions See also: explaining how to perform the optimization procedure.
  • Page 50 3 Preparing the System Rotate the camera’s focusing ring, shown below, to focus the camera on the sprayer tip. Focusing the camera: Reference probe (present only for ToF-variant ionKey sources, for use on instruments fitted with a time-of-flight (ToF) mass analyzer) Camera’s focusing ring December 8, 2014, 715004028 Rev.

  • Page 51: Adjusting The Position Of The Sprayer Tip

    Optimizing the source Adjusting the position of the sprayer tip Requirements: • Solvent composition affects the optimal capillary voltage. Infuse an optimization solution of the same solvent composition as the point in the gradient at which your analytes will elute. For example, if the majority of analytes elute at 25% organic solvent, use an optimization solution of 25% organic solvent.
  • Page 52 3 Preparing the System To adjust the sprayer tip position: Pull open the ionKey source door from the top of the source enclosure. Adjusting the sprayer tip position: X scale X control Y control Y scale Z scale Z control While viewing the camera control, adjust the x, y, and z thumbscrew controls on the adjuster assembly to move the sprayer tip relative to the sampling cone, and adjust for optimal signal.
  • Page 53 Optimizing the source Focusing on the sprayer tip: Sample cone Optimal position of sprayer tip Recommendations: • A suggested starting range for the capillary voltage is approximately 3.0 kV to 3.8 kV. Both position and flow rate affect the optimal voltage. The higher the flow rate, the greater the optimal voltage.
  • Page 54 3 Preparing the System Tips: • Fine tune the position of the sprayer while acquiring a spectrum of a standard compound. Small adjustments to the sprayer position can make large differences to the source sensitivity. • If spray instability occurs, adjust the sprayer position and capillary voltage.
  • Page 55 Optimizing the source source. If arcing is present, you must adjust the emitter position away from the grounding point. • Ensure that the baffle does not strike the emitter or the reference-probe TaperTip emitter in the Sample or LockSpray position. If necessary, readjust the position of the reference probe. Baffle in Sample position: Sample cone Reference-probe...
  • Page 56 3 Preparing the System Close the door of the ionKey source. 10. Flush the infusion iKey separation device with water. 11. Stop the flow, and then remove the infusion iKey separation device (follow the procedure on page 47). December 8, 2014, 715004028 Rev. B...

  • Page 57: Analyzing Samples

    • The console software’s online Help, for detailed information about installing samples in the µSample manager. • www.waters.com, for detailed information about vials and plates. Inserting an analytical iKey separation device “Inserting the iKey separation device” on page See also:...

  • Page 58: Preparing For Sample Analysis

    3 Preparing the System Preparing for sample analysis To prepare for sample analysis: Create an instrument method for the µBinary solvent manager, adopting a direct-injection method. For trapping mode, create a direct-injection method and a Requirement: trapping method. On the analytical tab, select the A and B solvents, specify the seal-wash duration and the steps in the gradient table.

  • Page 59: Typical Settings For Analysis

    The EEPROM in the iKey separation device stores information such as usage and the device’s history of sample sets. Typical settings for analysis For typical analytical settings, refer to the guide Waters Customer Familiarization Method or Installation Test Method supplied with your instrument.
  • Page 60 3 Preparing the System December 8, 2014, 715004028 Rev. B...

  • Page 61: Maintenance Procedures

    Maintenance Procedures The maintenance guidelines and procedures in the following guides are necessary to maintain the performance of the system. Keep to a maintenance schedule, and perform maintenance as required and described in these guides: • ACQUITY UPLC M-Class System Guide. •...

  • Page 62: Spare Parts

    4 Maintenance Procedures Spare parts To ensure that your system operates as designed, use only Waters Quality ® Parts . Visit www.waters.com/wqp for information about Waters Quality Parts, including how to order them. Safety and handling Bear in mind the following safety considerations when performing...
  • Page 63 Safety and handling To avoid damaging the iKey separation device, observe these Notice: precautions: • Handle the device with care; the component parts are fragile. • Do not subject the device to pressures that exceed 69,000 kPa (690 bar, 10,000 psi). •...

  • Page 64: Testing The Integrity Of The Solvent Flow Path

    4 Maintenance Procedures Testing the integrity of the solvent flow path You can assess the integrity of the solvent flow path, which extends through the various modules of the system. To do so, you perform tests using the diagnostic iKey separation device. To test flow through the system, for direct injection, perform the fluidic integrity test.

  • Page 65: Mass Spectrometer Maintenance Procedures

    Mass spectrometer maintenance procedures Mass spectrometer maintenance procedures The overview and maintenance guides of all mass spectrometers include a chapter named “Maintenance Procedures”, which provides maintenance guidelines and procedures necessary to maintain an instrument’s performance. These procedures relate particularly to the ionKey source: •...
  • Page 66 4 Maintenance Procedures December 8, 2014, 715004028 Rev. B...

  • Page 67: A Safety Advisories

    Topic Page Warning symbols................68 Notices ....................72 Warnings that apply to all Waters instruments and devices ..73 Warnings that address the replacing of fuses ........ 78 Electrical and handling symbols ............. 80 December 8, 2014, 715004028 Rev. B...

  • Page 68: Warning Symbols

    The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device, or a component of an instrument or device. When one of these symbols appear in a manual’s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid it.

  • Page 69: Specific Warnings

    Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. To avoid injury from bursting, nonmetallic tubing, heed these Warning:...
  • Page 70 A Safety Advisories Mass spectrometer shock hazard The following warning applies to all Waters mass spectrometers. To avoid electric shock, do not remove the mass spectrometer’s Warning: protective panels. The components they cover are not user-serviceable. The following warning applies to certain mass spectrometers when they are in Operate mode.
  • Page 71 Warning symbols Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans. To avoid infection with potentially infectious, human-sourced...

  • Page 72: Notices

    A Safety Advisories Notices Notices appear where an instrument or device can be subject to use or misuse that can damage it or compromise a sample’s integrity. The exclamation point symbol and its associated statement alert you to such risk. To avoid damaging the instrument’s case, do not clean it with Notice: abrasives or solvents.

  • Page 73: Warnings That Apply To All Waters Instruments And Devices

    Warnings that apply to all Waters instruments and devices Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Changes or modifications to this unit not expressly approved by the Attention: party responsible for compliance could void the user’s authority to operate the...
  • Page 74 A Safety Advisories Use caution when working with any polymer tubing under pressure: Warning: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. •...
  • Page 75 Warnings that apply to all Waters instruments and devices fare attenzione quando si utilizzano tubi in materiale polimerico Attenzione: sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante.
  • Page 76 A Safety Advisories 警告：当有压力的情况下使用管线时，小心注意以下几点： • 当接近有压力的聚合物管线时一定要戴防护眼镜。 • 熄灭附近所有的火焰。 • 不要使用已经被压瘪或严重弯曲的管线。 • 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。 • 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。 경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오. • 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오. • 근처의 화기를 모두 끄십시오. • 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오. •...
  • Page 77 Warnings that apply to all Waters instruments and devices The user shall be made aware that if the equipment is used in a Warning: manner not specified by the manufacturer, the protection provided by the equipment may be impaired. L’utilisateur doit être informé que si le matériel est utilisé d’une Attention: façon non spécifiée par le fabricant, la protection assurée par le matériel risque...

  • Page 78: Warnings That Address The Replacing Of Fuses

    A Safety Advisories Warnings that address the replacing of fuses The following warnings pertain to instruments equipped with user-replaceable fuses. If the fuse types and ratings appear on the instrument: To protect against fire, replace fuses with those of the type Warning: and rating printed on panels adjacent to instrument fuse covers.
  • Page 79 Warnings that address the replacing of fuses If the fuse types and ratings do not appear on the instrument: To protect against fire, replace fuses with those of the type Warning: and rating indicated in the “Replacing fuses” section of the Maintenance Procedures chapter.

  • Page 80: Electrical And Handling Symbols

    A Safety Advisories Electrical and handling symbols Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument’s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse...

  • Page 81: Handling Symbols

    Electrical and handling symbols Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped. Keep upright! Keep dry! Fragile! Use no hooks! December 8, 2014, 715004028 Rev. B...
  • Page 82 A Safety Advisories December 8, 2014, 715004028 Rev. B...

  • Page 83: B External Connections

    External Connections A Waters Technical Service representative unpacks and installs your ™ ionKey/MS system. ACQUITY UPLC M-Class Driver Pack Installation and See also: Configuration Guide Requirements: • Contact Waters Technical Service before moving the ACQUITY ® UPLC M-Class System. •...

  • Page 84: System Tubing Connections

    B External Connections System tubing connections The system’s external tubing connections for solvent flow and drainage are shown in the following diagram. December 8, 2014, 715004028 Rev. B...
  • Page 85 System tubing connections Solvent flow for ionKey/MS system: Solvent tray BSM solvents SM wash solvents Sample Manager - FL Sample syringe Needle To waste Sample Injector valve loop Wash port source Wash manifold Filter Flow sensor Waste Mixer Vent Flow...

  • Page 86: External Wiring Connections

    B External Connections External wiring connections ionKey/MS system external wiring connections The rear panel connections for ionKey/MS system modules are shown below: ionKey-compatible mass spectrometer ACQUITY UPLC M-Class ® (Xevo TQ-S shown) Solvent tray Trap valve manager (optional) Sample organizer...

  • Page 87: Ethernet Connections

    External wiring connections Ethernet connections The sample manager incorporates an internal 10/100/1000 megabit Ethernet switch that accommodates the PC (workstation) and as many as six system modules. Connect the shielded Ethernet cables from each module to the electronic connections on the rear panel of the sample manager. The sample manager is connected internally to the Ethernet switch.

  • Page 88: Signal Connections

    B External Connections Signal connections Making signal connections Refer to the signal connection location shown on the silk-screened label affixed to the rear panel of each system module. See also: • µBinary Solvent Manager Overview and Maintenance Guide • µSample Manager-Fixed Loop Overview and Maintenance Guide •...
  • Page 89 Signal connections Using the flat-blade screwdriver, attach the positive and negative leads of the signal cable to the connector. Screw Connector Signal cable Fit the grounding cable’s fork terminal on the rear panel grounding stud, and secure the terminal with the locking nut. Use the 9/32-inch nut driver to tighten the locking nut until the fork Tip: terminal does not move.
  • Page 90 B External Connections Binary solvent manager I/O signal connectors The rear panel of the binary solvent manager includes two removable connectors that hold the screw terminals for I/O signal cables. These connectors are keyed so that they can be inserted only one way. Binary solvent manager I/O signal connectors: Connector I Connector II...
  • Page 91 Signal connections Binary solvent manager analog-out/event-in connections: Signal connection Description Auxiliary 1 In Reserved for future use. Auxiliary 2 In Reserved for future use. Run Stopped Out Indicates (with a contact closure) the binary solvent manager has ceased operation because of an error condition or operator request.
  • Page 92 B External Connections Chart-out signal conditions: Parameter Setting at 0 Parameter Setting at Signal Volts (Minimum) 2.000 Volts (Maximum) Flow Rate 0.2 µL/min 100 µL/min System Pressure –345 kPa (–3.45 bar, 103,421 kPa (1034 bar, –50 psi) 15,000 psi) Composition 0.0% 100.0% Sample manager I/O signal connectors...
  • Page 93 Signal connections Sample manager event-out/event-in connections: Signal connections Description Inject Start Out Indicates (with a contact closure output) that an injection has started. Inject Hold In Delays the next injection when the sample manager receives a contact closure input (from another system module, for example).

  • Page 94: Connecting To The Electricity Source

    (or better) in Europe. The main power cord must be replaced only with one of adequate rating. For information regarding what cord to use in other countries, contact your local Waters distributor. • Power-off and unplug each system module before performing any maintenance operation on the module.
  • Page 95 Connecting to the electricity source FlexCart power connections: To circuit A AC line Network switch (if needed) Sample manager Binary solvent FlexCart manager power strips LCD/monitor Computer workstation Trap Valve Manager (optional) To circuit B AC line December 8, 2014, 715004028 Rev. B...
  • Page 96 B External Connections December 8, 2014, 715004028 Rev. B...

  • Page 97: C Materials Of Construction And Compatible Solvents

    Materials of Construction and Compatible Solvents This appendix describes the safety issues you must consider when parts of the system are exposed to solvents. To confirm the integrity of the source exhaust Warning: system, you must address any safety issues raised by the contents of this Appendix.

  • Page 98: Preventing Contamination

    C Materials of Construction and Compatible Solvents Preventing contamination For information about preventing contamination, refer to Controlling Contamination in UltraPerformance LC/MS and HPLC/MS Systems (part number 715001307), which you can download from the Waters Web site (www.waters.com). December 8, 2014, 715004028 Rev. B...

  • Page 99: Items Exposed To Solvent

    See the following section for details about the most common ingredients used to prepare mobile phases. Items exposed to solvent: Item Material ionKey/MS system Fused silica source, tubing, fittings, ™ PEEK solvent filters Alumina based ceramic with proprietary ™...
  • Page 100 C Materials of Construction and Compatible Solvents December 8, 2014, 715004028 Rev. B...

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