Page 2: General Information
This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com).
Page 3: Customer Comments
All other trademarks or registered trademarks are the sole property of their respective owners. Customer comments Waters’ Technical Communications organization invites you to report any errors that you encounter in this document or to suggest ideas for otherwise improving it. Help us better June 9, 2016, 715004990 Rev.
Page 4: Contacting Waters
We seriously consider every customer comment we receive. You can reach us at tech_comm@waters.com. Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information Contacting medium Information...
Page 5: Safety Considerations
For information regarding what cord to use in other countries, contact your local Waters distributor. Solvent leakage hazard The source exhaust system is designed to be robust and leak-tight. Waters recommends you perform a hazard analysis assuming a maximum leak into the laboratory atmosphere of 10% LC eluate.
Page 6 Prohibited: Do not place vessels containing liquid—such as solvent bottles—atop the workstation or ancillary equipment or otherwise expose those units to dripping or splashing liquids. Spilled solvents hazard Prohibited: To avoid equipment damage caused by spilled solvent, do not place reservoir bottles directly atop an instrument or device or on its front ledge.
Page 7 Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual June 9, 2016, 715004990 Rev.
Page 8: Electrical Power Safety Notice
contamination permissible. That authority must also prescribe the method of decontamination to be used and the appropriate protection for personnel undertaking the decontamination process. You must handle items such as syringes, fused silica lines, and borosilicate tips used to carry sample into the source area in accordance with laboratory procedures for contaminated vessels and sharps.
Page 9: Audience And Purpose
Intended use of the device Waters designed the Xevo TQ-XS for use as a research tool to accurately, reproducibly, and robustly quantify target compounds present at the lowest possible levels in highly complex sample matrices.
Page 10: Calibrating
When fitted with Waters options (APCI, APGC, APPI, ASAP, ESCi, NanoFlow ESI, TRIZAIC, UniSpray, UPLC, ionKey), or optional third-party sources (DART, DESI, or LDTD), the Xevo TQ- XS does not comply with the European Union In Vitro Diagnostic Device Directive 98/79/EC.
Page 11: Ism Classification: Ism Group 1 Class A
To avoid difficulties in ensuring electromagnetic compatibility, if the instrument's pump control cable is attached to the vacuum hose, ensure that the cable is grounded to the mass spectrometer. EC authorized representative Address Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone...
Page 12: Table Of Contents
1.1 IntelliStart technology........................20 1.2 ACQUITY UPLC/MS Xevo TQ-XS systems.................. 21 1.2.1 ACQUITY UPLC system...................... 21 1.2.2 Waters ACQUITY Xevo TQ-XS UPLC/MS system.............. 21 1.2.3 ACQUITY UPLC M-Class system..................22 1.2.4 Non-ACQUITY devices for use with the Xevo TQ-XS ............22 June 9, 2016, 715004990 Rev. A...
Page 13 1.2.5 Software and data system ....................23 1.3 Ionization techniques and source probes..................23 1.3.1 Electrospray ionization ......................24 1.3.2 ESCi............................. 24 1.3.3 APCI ............................ 24 1.3.4 Dual-mode APPI/APCI source..................... 24 1.3.5 UniSpray ..........................25 1.3.6 Low-flow ESI probe......................25 1.3.7 NanoFlow ESI source ......................25 1.3.8 Atmospheric solids analysis probe (ASAP) ................. 25 1.3.9 APGC...........................
Page 14 2.4.1 Power LED........................... 39 2.4.2 Operate LED........................40 2.5 Tuning and calibration information ....................40 2.6 Running the mass spectrometer at high flow rates ............... 40 2.7 Preparing the IntelliStart Fluidics system ..................41 2.7.1 Installing the reservoir bottles ....................41 2.7.2 Installing the low-volume vials ..................... 42 2.7.3 Adjusting the solvent delivery tube positions ...............
Page 15 3.5.1 Installing the NanoFlow source.................... 77 3.5.2 Fitting a borosilicate glass capillary (nanovial) ..............80 3.5.3 Positioning the borosilicate glass capillary tip..............83 3.5.4 Restarting a stalled borosilicate glass capillary electrospray..........83 3.6 ionKey source ..........................83 3.6.1 Installing the ionKey source....................84 3.6.2 Installing ionKey source software ..................88 3.6.3 Installing the camera in the ionKey source ................
Page 16 4.13.5 Fitting the sampling cone assembly to the source............110 4.14 Cleaning the ion block assembly....................111 4.14.1 Removing the ion block assembly from the source assembly ......... 111 4.14.2 Disassembling the source ion block assembly ..............114 4.14.3 Cleaning the ion block components................. 118 4.14.4 Assembling the source ion block assembly ..............
Page 17 A.1.1 Specific warnings ......................180 A.2 Notices............................182 A.3 Bottles Prohibited symbol ......................182 A.4 Required protection ........................182 A.5 Warnings that apply to all Waters instruments and devices ............183 A.6 Warnings that address the replacing of fuses................187 A.7 Electrical symbols ........................188 A.8 Handling symbols ........................189 B External connections.....................191...
Page 18 B.6 Connecting the nitrogen exhaust line ..................199 B.7 Connecting liquid waste lines ..................... 201 B.8 Connecting the workstation (systems with no ACQUITY LC)............. 204 B.8.1 Connecting to the workstation ................... 204 B.9 Connecting Ethernet cables (systems with ACQUITY LC) ............204 B.10 Input/output signal connectors....................205 B.11 Connecting to the electricity source..................
Page 19: Waters Xevo Tq-Xs Overview
Available source options can vary, depending on the software you use to operate the Xevo TQ- XS. Refer to the MassLynx or UNIFI online Help for more information about supported sources. For mass spectrometer specifications, see the Waters Xevo TQ-XS Site Preparation Guide (715005172).
Page 20: Intellistart Technology
IntelliStart technology. In Waters documents, the term “fluidics” refers to the IntelliStart Fluidics system, which is the instrument’s onboard system that delivers sample and solvent to the probe of the mass spectrometer. It can also denote plumbing components and fluid pathways within and between system modules.
Page 21: Acquity Uplc/Ms Xevo Tq-Xs Systems
1.2 ACQUITY UPLC/MS Xevo TQ-XS systems The Waters Xevo TQ-XS is compatible with the ACQUITY UPLC systems. If you are not using an ACQUITY UPLC system, refer to the documentation relevant to your LC system. 1.2.1 ACQUITY UPLC system The ACQUITY UPLC system includes a binary or quaternary solvent manager, sample manager, column heater or column manager, optional sample organizer, one or more detectors, a specialized ACQUITY UPLC column, and software to control the system.
Page 22: Acquity Uplc M-Class System
For further information, see the ACQUITY UPLC M-Class System Guide or Controlling Contamination in UltraPerformance LC/MS and HPLC/MS Systems (part number 715001307). You can find these documents on www.waters.com; click Services & Support > Support. 1.2.4 Non-ACQUITY devices for use with the Xevo TQ-XS The following non-ACQUITY LC devices are validated for use with the Xevo TQ-XS: •...
Page 23: Software And Data System
• Waters 2998 PDA detector • Waters 2487 UV detector • Waters 1525µ binary gradient pump + 2777 autosampler 1.2.5 Software and data system You can use MassLynx software v4.2 to control the mass spectrometer. The software enables these major operations: •...
Page 24: Electrospray Ionization
1.3.1 Electrospray ionization In electrospray ionization (ESI), a strong electrical charge is applied to the eluent as it emerges from a nebulizer. The droplets that compose the resultant aerosol undergo a reduction in size (solvent evaporation). As solvent continues to evaporate, the charge density increases until the droplet surfaces eject ions (ion evaporation).
Page 25: Unispray
1.3.5 UniSpray The UniSpray source enables the detection of a wide range of compounds in a single analysis. In contrast to Electrospray ionization, UniSpray uses a grounded capillary, and the resulting spray is directed at an impactor pin held at a voltage, creating smaller charged droplets, amenable to easy desolvation.
Page 26: Apgc
See the Atmospheric Solids Analysis Probe Operator's Guide Supplement for further details. 1.3.9 APGC The Waters APGC couples an Agilent GC with the Xevo TQ-XS. Doing so enables you to perform LC and GC analyses in the same system, without compromising performance. The APGC provides complementary information to the LC/MS instrument, enabling analysis of compounds of low molecular weight and low-to-intermediate polarity.
Page 27 For further information on the IntelliStart fluidics system, see IntelliStart Fluidics Plumbing and the diagram located on the inside of the fluidics access door (see Waters ACQUITY Xevo TQ-XS UPLC/MS system). Figure 1–3: IntelliStart fluidics system: Reservoir C...
Page 28: System Components
1.4.2 System components The onboard system incorporates a 7-port selector valve, a multi-position diverter valve, a pump, and three sample reservoirs. The sample reservoirs are mounted on the instrument’s front panel. When you select a solvent from the instrument console, an LED illuminates the appropriate reservoir. You can simultaneously illuminate all three reservoirs or extinguish the LEDs for light-sensitive samples.
Page 29: Ms Operating Modes
Quadrupole 1 (MS1) T-Wave/ScanWave collision cell Quadrupole 2 (MS2) Conversion dynode Detector assembly Photomultiplier tube Source sampling orifice Isolation valve Z-Spray ion source Sample inlet Sample cone Conjoined ion guide StepWave T-Wave ion guide 1.6 MS operating modes The following table shows the MS operating modes. June 9, 2016, 715004990 Rev.
Page 30: Ms/Ms Operating Modes
Table 1–1: MS operating modes: Operating mode Collision cell Pass all masses Resolving (scanning) Pass all masses Resolving (static) Resolving (scanning) Pass all masses In MS mode, the instrument can acquire data at scan speeds as high as 20,000 Da/s. Use this mode for instrument tuning and calibration before MS/MS analysis.
Page 31: Product (Daughter) Ion Mode
RADAR is an additional mode with which you simultaneously collect data from the MRM and full scan MS modes. RADAR mode can also acquire all detectable ions in both positive and negative full scan MS. 1.7.1 Product (daughter) ion mode Product ion mode is the most commonly used MS/MS operating mode.
Page 32: Mrm Mode
MS1 - Scanning Collision cell - Fragment precursor ions and pass all masses MS2 - Static (at product mass) 1.7.2.1 Typical application You typically use the precursor ion mode for structural elucidation—that is, to complement or confirm product scan data—by scanning for all the precursors of a common product ion. 1.7.3 MRM mode MRM mode is the highly selective MS/MS equivalent of SIR.
Page 33: Constant Neutral Loss Mode
1.7.4 Constant neutral loss mode Constant neutral loss mode detects the loss of a specific neutral fragment or functional group from an unspecified precursor or precursors. The scans of MS1 and MS2 are synchronized. When MS1 transmits a specific precursor ion, MS2 determines whether that precursor loses a fragment of a certain mass.
Page 34: Leak Sensors
1.5 mL of accumulated leaked liquid in its surrounding reservoir. At the same time, the software displays an error message alerting you that a leak has developed. Consult the Waters ACQUITY UPLC Leak Sensor Maintenance Instructions (part number 71500082506) for complete details.
Page 35: Rear Panel
The system monitors the turbomolecular pump speeds and continuously measures vacuum pressure with built-in Pirani and Penning gauges. The gauges also serve as switches, stopping operation when vacuum loss is detected. A vacuum isolation valve isolates the source sample cone from the mass analyzer, allowing you to perform routine maintenance without venting the system.
Page 36 Source vent Nitrogen inlet Pilot valve port Turbo vacuum Source vacuum Collision cell gas inlet June 9, 2016, 715004990 Rev. A Page 36...
Page 37: Preparing The Mass Spectrometer For Operation
Preparing the mass spectrometer for operation This chapter describes how to start and shut down the mass spectrometer. 2.1 Preparing to start the mass spectrometer This instrument is compatible with the ACQUITY UPLC system; if you are not using an ACQUITY UPLC system, refer to the documentation relevant to the system you are using (see Non- ACQUITY devices for use with the Xevo...
Page 38: Starting The Mass Spectrometer
Requirement: The collision gas is argon; it must be dry and of high purity (99.997%). Regulate the supply at 50 kPa (0.5 bar, 7 psi). 2.2 Starting the mass spectrometer Starting the mass spectrometer comprises powering-on the workstation, logging in, powering-on the mass spectrometer and all other instruments, and then starting the software.
Page 39: Verifying The Instrument's State Of Readiness
Tips: • In the Instrument Console, the System Ready indicator shows green when the instrument is fully pumped-down (evacuated). • Expect the Analyzer Penning gauge readback to show less than 1e-5 mbar vacuum. Warning: To prevent the ignition of flammable solvent vapors in the enclosed space of a mass spectrometer’s ion source, ensure that these conditions are met: •...
Page 40: Operate Led
2.4.2 Operate LED The Operate LED, located on the right-hand side of the power LED, indicates the operating condition. See the mass spectrometer’s online Help topic “Monitoring the mass spectrometer LEDs” for details on the Operate LED indications. 2.5 Tuning and calibration information You must tune and, if necessary, calibrate the instrument prior to use.
Page 41: Preparing The Intellistart Fluidics System
2.7 Preparing the IntelliStart Fluidics system For additional information, see Connecting liquid waste lines. Prohibited: To avoid equipment damage caused by spilled solvent, do not place reservoir bottles directly atop an instrument or device or on its front ledge. Instead, place the bottles in the bottle tray, which serves as secondary containment in the event of spills.
Page 42: Installing The Low-Volume Vials
2.7.2 Installing the low-volume vials Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. To install low-volume vials: If a standard reservoir bottle is fitted, remove it. Screw the low-volume adaptors into the manifold and finger-tighten them. Figure 2–2: Low-volume adaptor Low-volume vial...
Page 43: Purging The Fluidics
To adjust the position of a solvent delivery tube: Open the access door to the fluidics pump (see Waters ACQUITY Xevo TQ-XS UPLC/MS system). Loosen the finger-tight fitting for the solvent delivery tube you are adjusting. Finger-tight fitting Solvent delivery tube Move the solvent delivery tube so that its end is close to, but does not touch, the bottom of the reservoir bottle or low volume vial.
Page 44: Leaving The Mass Spectrometer Ready For Operation
• The software fails to establish communication or loses communication with the mass spectrometer. • You perform a software upgrade. To reboot the mass spectrometer: Ensure that the mass spectrometer software is closed. Open the mass spectrometer’s front, left-hand door. Insert a short length (7.5 cm) of PEEK tubing, or similar object, into the reset button aperture to operate the reset button.
Page 45: Emergency Shutdown Of The Mass Spectrometer
Notice: For ionKey operation, to protect the iKey when you leave the mass spectrometer in Operate mode with no flow, set the capillary voltage to zero. 2.11 Emergency shutdown of the mass spectrometer Warning: To avoid electric shock, observe the following procedure to isolate the instrument from the main power supply.
Page 46: Changing The Mode Of Operation
• UniSpray • NanoFlow ESI • ionKey source For details about other Waters and third-party source options, refer to the documentation supplied with the source. 3.2 ESI, ESCi, and APCI modes ESI, ESCi, and APCI modes are all configured using a standard source enclosure.
Page 47: Esci Mode
3.2.2 ESCi mode To operate in ESCi mode, you must fit an ESI probe adaptor and corona pin to the source enclosure. The system, with the ESI probe adaptor installed and corona discharge pin fitted, can alternate between ESI and APCI ionization modes, facilitating data acquisition in ESI and APCI modes in parallel.
Page 48 Figure 3–2: Probe adaptor types APCI probe adaptor APCI label APCI probe heater ESI probe tip ESI label ESI probe adaptor June 9, 2016, 715004990 Rev. A Page 48...
Page 49 Figure 3–3: Probe assembly Probe inlet PEEK fitting Identification label for part number Capillary Probe adaptor PEEK fitting For ESCi and APCI modes, you must also install a corona pin. Table 3–1: Configuration for ESI/ESCi/APCI modes Mode Probe adaptor Install corona pin ESCi APCI APCI...
Page 50: Installing The Probe Adaptor
3.2.5 Installing the probe adaptor Figure 3–4: Probe adaptor parts Probe adaptor cap removed from probe adaptor Probe adaptor cap tether Locking ring Probe adaptor identification label Probe adaptor cap release buttons Required materials • Chemical-resistant, powder-free gloves To install the probe adaptor: Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure.
Page 51 To prepare for installing a probe assembly, press together the probe-adaptor-cap release buttons, in the direction shown by the arrows in the following figure, and lift the probe adaptor cap off the probe adaptor. Figure 3–5: Probe adaptor cap release For ESI probe adaptors, remove the protective cap, if fitted, from the probe tip.
Page 52 Probe location dowel Figure 3–8: Locating the ESI probe adaptor Location hole for probe location dowel Probe adjuster assembly Figure 3–9: Locating the APCI probe adaptor Location hole for probe location dowel Probe adjuster assembly Rotate the probe adaptor locking ring clockwise to secure the probe adaptor in place. June 9, 2016, 715004990 Rev.
Page 53: Installing The Probe Assembly
Figure 3–10: Probe adaptor, mounted on the source enclosure: Probe adaptor cap tether Probe adaptor cap Source enclosure Probe adjuster assembly High voltage connector ESI probe adaptor cable (ESI probe adaptor only) Probe adaptor locking ring For ESI probe adaptors, connect the ESI probe adaptor’s cable to the high voltage connector.
Page 54 • Ensure that you use a probe assembly appropriate for your application. For example, using an ESI probe assembly with an APCI probe adaptor compromises instrument performance. You can use the part number on the identification label to verify the probe assembly type. •...
Page 55 Warning: To avoid electric shock, do not insert any item into the probe cap aperture when the probe cap is fitted to the instrument. Notice: To avoid damaging the probe assembly, take care when inserting the capillary into the probe adaptor. Do not use force. Warning: To avoid harmless, static-like electric shock, ensure the mass spectrometer is in Standby mode before you touch any of its external surfaces that are marked with...
Page 56 Figure 3–13: Probe assembly fitted to the probe adaptor Probe adaptor fitting Tip: The probe adaptor fitting on the UniSpray probe assembly is not compatible with the probe adaptor. If you cannot fit the probe adaptor cap, ensure that you are installing the correct probe assembly.
Page 57 Figure 3–15: Probe adaptor cap Probe adaptor cap tether Probe adaptor Probe assembly tubing Slide the probe adaptor cap along the probe assembly, over the probe adaptor PEEK fitting. Push the probe adaptor cap on to the probe adaptor until it clicks. Tips: •...
Page 58 Figure 3–16: Seating the probe adaptor cap Probe adaptor cap seated incorrectly; edge does not align with the edge of the probe adaptor Probe adaptor cap seated correctly; edge aligns with the edge of the probe adaptor If you are not immediately connecting the probe assembly to the fluidics, insert the probe inlet PEEK fitting in to the PEEK fitting holder.
Page 59 Probe assembly tubing Warning: To avoid electric shock or solvent ignition, when connecting ESI or source probes directly to non-Waters equipment, ensure that the liquid outlet connection is grounded. To connect the probe inlet PEEK fitting to the IntelliStart Fluidics system:...
Page 60: Removing The Probe Adaptor
Figure 3–18: Tubing connection between the diverter valve and the ESI probe Tip: The other plumbing connections are omitted for clarity. Probe adaptor cap PEEK fitting holder Leak tray Probe adaptor Probe inlet PEEK fitting connected to diverter valve Tubing Close the access door to the IntelliStart Fluidics system.
Page 61: Installing And Removing The Corona Pin
Warning: To avoid electric shock or solvent ignition, when connecting ESI or source probes directly to non-Waters equipment, ensure that the liquid outlet connection is grounded. If the probe assembly is fitted, open the access door to the IntelliStart Fluidics system (see...
Page 62 3.2.8.1 Installing the corona pin in the source Required materials Chemical-resistant, powder-free gloves To install the corona pin in the source: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Warning: To avoid harmless, static-like electric shock, ensure the mass spectrometer is in Standby mode before you touch any of its external surfaces that are marked with...
Page 63 Warning: To avoid puncture wounds, handle sharp parts and materials with care. Fit the corona pin to the corona pin mounting contact, ensuring that the corona pin is securely mounted and that its tip aligns with the sample cone orifice. Figure 3–20: Corona pin: Corona pin Close the source enclosure.
Page 64 Figure 3–21: Source enclosure Source window Vernier probe adjuster 3.2.8.2 Removing the corona pin from the source Required materials Chemical-resistant, powder-free gloves To remove the corona pin from the source: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure.
Page 65: Combined Appi/Apci Source
Prepare the instrument for working on the source (see Prepare the instrument for working on the source). Warning: To avoid burn injuries, take great care while performing this procedure. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if an ESI probe is fitted;...
Page 66: Apci Operation
Sample molecules APPI lamp drive assembly Repeller electrode UV lamp Photons from the UV lamp Sample cone Sample ions APCI probe The APCI probe introduces vaporized sample into the source where photons generated by an ultra-violet (UV) lamp (mounted in the APPI lamp drive assembly) produce sample ions. Direct photoionization of a sample molecule occurs when the photon energy exceeds the ionization potential of the sample molecule.
Page 67: Dual-Mode Operation
Sample cone APCI probe The APCI probe introduces vaporized sample into the source. The sample passes between the sample cone and the corona pin, which typically operates with a discharge current of 5 µA. The corona discharge generates ions that react with the mobile phase molecules to produce stable reagent ions.
Page 68: The Combined Appi/Apci Source Components
Figure 3–25: Dual operation in APPI mode: Repeller electrode with voltage applied Corona pin inactive Photons from the UV lamp Sample cone APCI probe 3.3.4 The combined APPI/APCI source components The combined APPI/APCI source comprises the APCI probe adaptor and a source enclosure with an APPI lamp drive incorporated.
Page 69: Installing The Combined Appi/Apci Source
APPI lamp drive assembly Notice: To prevent damage to the corona pin and lamp assembly, ensure that the lamp assembly does not touch the corona pin when the source enclosure door is closed. The UV lamp, which you ignite via a control in the MassLynx software Tune window, provides a constant photon output.
Page 70: Removing The Combined Appi/Apci Source Enclosure
Install the specially shaped corona pin (see Installing the corona pin in the source). Slide open the instrument’s source interface door (see the figure on Waters ACQUITY Xevo TQ-S UPLC/MS system). Connect the APPI drive cable to the instrument’s front panel connector.
Page 71: Unispray Source
Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Warning: To avoid electric shock, ensure that the instrument is prepared for working on the source before commencing this procedure. Warning: To avoid burn injuries, before performing maintenance operations that involve handling components inside the mass spectrometer's ion source, allow the source interior to cool.
Page 72 Figure 3–28: UniSpray source - front and rear view June 9, 2016, 715004990 Rev. A Page 72...
Page 73: Installing The Unispray Source
Probe inlet shaft Vertical probe adjuster Horizontal probe adjuster Impactor pin Capillary adjuster Source enclosure front panel See also: For information about maintaining the source components: • Replacing the UniSpray probe assembly • Maintaining the UniSpray impactor pin 3.4.1 Installing the UniSpray source Required materials •...
Page 74 Tip: If you must fit the probe assembly, do so by inserting the probe assembly into the probe inlet shaft atop the source, and screwing the probe fitting into the probe inlet. See Fitting the UniSpray probe assembly. Using two hands, fit the UniSpray source enclosure to the two supporting studs on the adaptor housing.
Page 75 Figure 3–30: UniSpray source connections Probe adjuster cable (yellow) High-voltage connector Slide open the instrument's source interface door. Figure 3–31: UniSpray Source connections to mass spectrometer June 9, 2016, 715004990 Rev. A Page 75...
Page 76: Removing The Unispray Source
Instrument source interface door High-voltage cable socket Probe adjuster cable socket (yellow) Connect the high-voltage cable to the high-voltage cable socket on the mass spectrometer. Connect the probe adjuster cable to the probe adjuster cable socket on the mass spectrometer. Close the instrument's source interface door.
Page 77: Nanoflow Esi Source
3.5 NanoFlow ESI source The NanoFlow source enclosure comprises the NanoFlow stage (for x-, y-, z-axis adjustment), the sprayer-enclosure, and a microscope camera. Figure 3–32: NanoFlow source, stage and microscope camera: Microscope camera Sprayer enclosure X, Y, Z stage A sprayer is mounted on an X, Y, Z stage (three-axis manipulator), which slides on a pair of guide rails that allow its withdrawal from the source enclosure for maintenance and changes.
Page 78 Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Warning: To avoid electric shock, ensure that the instrument is prepared for working on the source before commencing this procedure. Warning: To avoid burn injuries, before performing maintenance operations that involve handling components inside the mass spectrometer's ion source, allow the source interior to cool.
Page 79 Fitting a borosilicate glass capillary (nanovial) • Capillary Electrophoresis and Capillary Electrochromatography Sprayer Operator's Guide (part number 6666522) Open the instrument’s source interface door (see Waters ACQUITY Xevo TQ-XS UPLC/MS system). Connect the probe cable to the instrument’s PROBE connection.
Page 80: Fitting A Borosilicate Glass Capillary (Nanovial)
3.5.2 Fitting a borosilicate glass capillary (nanovial) Required materials: • Chemical-resistant, powder-free gloves • Needle-nose pliers • Borosilicate glass capillary • Fused silica syringe needle or GELoader tip • Fused silica tubing cutter To fit a borosilicate glass capillary (nanovial): Warning: To avoid lacerations, puncture injuries, and possible contamination with biohazardous and toxic samples, do not touch the sharp end of the capillary.
Page 81 Figure 3–35: Capillary Union Remove the existing capillary from the sprayer. Carefully remove the new borosilicate glass capillary from its case by lifting it vertically while pressing on the foam with two fingers. Figure 3–36: Capillary Foam Load sample into the capillary using a fused silica syringe needle or a GELoader tip, minimizing any bubbles between the capillary tip and the sample.
Page 82 11. Finger-tighten the nut so that five mm of glass capillary protrudes from its end. Tip: Measure the protrusion from the end of the nut to the shoulder of the glass capillary. Figure 3–37: Sprayer Assembly: PTFE tubing Ferrule Union Knurled nut Blue conductive elastomer Glass capillary...
Page 83: Positioning The Borosilicate Glass Capillary Tip
3.5.3 Positioning the borosilicate glass capillary tip Having obtained a signal, you must adjust the tip position to maximize it. Using the three-axis manipulator, you can adjust the tip position up and down, left and right, forward and backward. As a starting point, set the tip so that it is on the center line of the sampling cone and at a distance between two and three times the diameter of the cone aperture.
Page 84: Installing The Ionkey Source
3.6.1 Installing the ionKey source The ionKey source enclosure comprises the iKey docking port, the iKey locking handle, the sprayer-enclosure, and a microscope camera. Figure 3–39: ionKey source: Microscope camera Handle for locking and unlocking the iKey separation device Front cover Docking port for the iKey separation device Required materials: •...
Page 85 Warning: To avoid burn injuries, exercise care when handling the components of the source enclosure heated to high temperatures. Wait until the hot components have sufficiently cooled before you handle them. Remove the existing source enclosure (see Removing the source enclosure from the instrument).
Page 86 Connect the data/power cable to the PSPI connector on the rear of the µSample manager, and use a screwdriver to firmly tighten the connector screws. Figure 3–41: Source connections to mass spectrometer: Data/power cable to PSPI µSample manager High-voltage cable Options cable Connect the high-voltage cable (white) to the high-voltage supply outlet on the mass spectrometer.
Page 87 Figure 3–42: Fluid line aperture: Aperture closed Aperture open (spring-loaded) Fluid line aperture Figure 3–43: µSample manager injection valve: Fluid inlet line connected to injection valve port 6 Connect the fluid inlet line to port 6 on the injection valve of the µSample manager. 10.
Page 88: Installing Ionkey Source Software
3.6.2 Installing ionKey source software If you are installing an ionKey source on your Xevo TQ-XS for the first time, you must install the appropriate MassLynx software SCN and the ACQUITY UPLC M-Class driver pack. For further details, see the following documents: •...
Page 89 Alternative: If you are using the ionKey source with an ACQUITY UPLC M-Class system mounted on an M-Class cart fitted with an ionKey or universal source holder, you can secure the source enclosure to the holder. Doing so keeps the enclosure close to the Xevo TQ-XS, for when it is next needed.
Page 90 Swing open the ionKey source enclosure unit from the source mounting on the mass spectrometer. Disconnect the high-voltage cable (white) from the high-voltage supply outlet on the mass spectrometer. 10. Disconnect the reference probe power cable (green) from the reference probe power inlet on the mass spectrometer.
Page 91: Maintenance Procedures
Maintenance procedures This section provides the maintenance guidelines and procedures necessary to maintain the mass spectrometer's performance. Keep to a maintenance schedule, and perform maintenance as required and described in this section. 4.1 Maintenance schedule The following table lists periodic maintenance schedules that ensure optimum instrument performance.
Page 92 Replace the source Annually. Replacing the source assembly seals. assembly seals. Replace the roughing pump. Every 3 years. Contact Waters. Replace the air filters. Annually. Replacing the air filter inside the front door. Clean the APPI/APCI When the window becomes APPI/APCI source—...
Page 93: Spare Parts
4.2 Spare parts Waters recommends that you replace only the parts mentioned in this document. For spare parts details, see the Waters Quality Parts Locator on the Waters Web site’s Services & Support page (http://www.waters.com/waters/en_US/Spare-Parts/nav.htm?cid=511444). 4.3 Troubleshooting with Connections INSIGHT Connections INSIGHT is an intelligent device management (IDM) Web service that enables Waters to provide proactive service and support for the ACQUITY UPLC system.
Page 94: Safety And Handling
4.4 Safety and handling Bear in mind the following safety considerations when performing maintenance procedures: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials.
Page 95: Preparing The Instrument For Working On The Source
Notice: To avoid damaging the iKey: • Handle it with care. The component parts are fragile. • For recommendations regarding the maximum pressure to subject the device to, see the iKey Separation Device Care and Use Manual (part number 720004897EN). •...
Page 96: Removing And Refitting The Source Enclosure
Remove the probe adaptor from the source (Removing the probe adaptor). Slide open the instrument’s source interface door (see Waters ACQUITY Xevo TQ-S UPLC/MS system). June 9, 2016, 715004990 Rev. A Page 96...
Page 97 Disconnect the probe adjuster and options cables from the instrument’s connectors. Warning: To avoid puncture wounds, handle sharp parts and materials with care. Notice: To avoid damaging the sample inlet, when removing a NanoLockSpray source enclosure, you must slide the sprayer platform out of the source enclosure before you open the enclosure.
Page 98: Fitting The Source Enclosure To The Instrument
4.6.2 Fitting the source enclosure to the instrument Required materials Chemical-resistant, powder-free gloves To fit the source enclosure to the instrument: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Warning: To avoid puncture injuries, handle sample needles, syringes, fused silica lines, and borosilicate tips with extreme care.
Page 99: Closing The Source Isolation Valve
4.7.1 Closing the source isolation valve To close the source isolation valve before starting a maintenance procedure: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Prepare the instrument for working on the source (see Preparing the instrument for working on the source).
Page 100: Opening The Source Isolation Valve
4.7.2 Opening the source isolation valve To open the source isolation valve after completing a maintenance procedure: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Warning: To avoid puncture wounds, take great care working with the source enclosure open if one or both of these conditions apply: •...
Page 101: Cleaning The Instrument Case
Figure 4–4: O-ring removal kit: Tool 1 Tool 2 To remove an O-ring: Note: To avoid damaging the component when removing an O-ring or seal from it, ensure that you do not scratch the component with the removal tool. Use the tools as aids to pull the O-ring or seal from its groove. Tip: If the O-ring or seal will not be reused, you can use the forked end of tool 2 to impale the O- ring or seal, aiding its removal.
Page 102 Figure 4–5: Nitrogen exhaust trap bottle: To laboratory exhaust port From instrument pilot valve port Bottle support Nitrogen exhaust trap bottle From instrument exhaust connection One-way valve Required materials Chemical-resistant, powder-free gloves To empty the nitrogen exhaust trap bottle: In the instrument console, click Stop Flow Pull the source enclosure release (located at the bottom, right-hand side) outward, and swing open the enclosure.
Page 103: Maintaining The Roughing Pump
4.11 Maintaining the roughing pump There are no user maintenance procedures for the EBARA EV-SA30 pump. To replace the pump, contact Waters Technical Support. 4.12 Cleaning the source components Clean the sample cone and cone gas nozzle when these conditions apply: •...
Page 104: Removing The Sampling Cone Assembly From The Source
4.13.1 Removing the sampling cone assembly from the source Required materials Chemical-resistant, powder-free gloves To remove the sampling cone assembly from the source: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Warning: To avoid electric shock, ensure that the instrument is in Standby mode before commencing this procedure.
Page 105: Disassembling The Sampling Cone Assembly
Figure 4–7: Ion block assembly Notice: To avoid damaging the StepWave assembly, do not switch the instrument into Operate mode when the isolation valve is closed. Notice: To avoid damage, do not open the source isolation valve before fitting the sampling cone assembly to the ion block assembly 4.13.2 Disassembling the sampling cone assembly Required materials •...
Page 106 Figure 4–8: Cone extraction tool location Combined 2.5-mm hex wrench and cone extraction Slide the collar to the end of the tool. Figure 4–9: Cone extraction tool Collar Insert the collar in the sample cone. Figure 4–10: Inserting the cone extraction tool Insert the collar Notice: To avoid damaging the sampling cone, which is fragile, do not place it...
Page 107 Rotate and lift the tool and collar to remove the sample cone from the cone gas nozzle. Figure 4–11: Removing the sample cone Rotate the tool Remove the sample cone Remove the O-ring from the sample cone. Figure 4–12: O-ring removed from the sample cone Sample cone O-ring Cone gas nozzle handle...
Page 108: Cleaning The Sample Cone And Cone Gas Nozzle
Warning: To avoid spreading contamination, dispose of all waste materials according to local environmental regulations. If the O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. Unscrew and remove the PEEK cone gas nozzle handle from the cone gas nozzle. 4.13.3 Cleaning the sample cone and cone gas nozzle Required materials •...
Page 109: Assembling The Sampling Cone Assembly
Place the vessels in the ultrasonic bath for 30 minutes. If you used formic acid in the cleaning solution, do as follows: Rinse the components by immersing them in separate glass vessels containing water and then placing the vessels in the ultrasonic bath for 20 minutes. Remove any residual water from the components by immersing them in separate glass vessels containing methanol and then placing the vessels in the ultrasonic bath for 10 minutes.
Page 110: Fitting The Sampling Cone Assembly To The Source
Fit the cone gas nozzle handle to the cone gas nozzle and turn the handle clockwise to tighten. Figure 4–13: Cone gas nozzle handle Cone gas nozzle Sample cone O-ring Carefully fit the sample cone into the cone gas nozzle. Fit the O-ring (a new one, if you disposed of the old O-ring) into the groove created between the sample cone and cone gas nozzle.
Page 111: Cleaning The Ion Block Assembly
Ensure that the source isolation valve is in the closed position (see Closing the source isolation valve). Hold the sampling cone assembly so that the cone gas nozzle handle is oriented horizontally and at the top, and then slide the sampling cone assembly into the ion block assembly.
Page 112 To remove the ion block assembly: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Vent and shut down the mass spectrometer (see the mass spectrometer’s online Help for details). Warning: To avoid personal injury, as well as damage to the roughing pump and mass spectrometer, disconnect the power cords for the mass spectrometer...
Page 113 Figure 4–15: Ion block assembly securing screws Remove the ion block assembly from the PEEK ion block support. Figure 4–16: PEEK ion block support Ion block assembly June 9, 2016, 715004990 Rev. A Page 113...
Page 114: Disassembling The Source Ion Block Assembly
4.14.2 Disassembling the source ion block assembly Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm hex wrench and cone extraction tool • O-ring removal kit To disassemble the ion block assembly: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure.
Page 115 • do not remove the sampling cone assembly retaining blocks. • do not adjust the screws securing the sampling cone assembly retaining blocks. Figure 4–18: Source ion block cover plate Ion block cover plate securing screw Ion block cover plate Remove the ion block cover plate.
Page 116 Warning: To avoid spreading contamination, dispose of all waste materials according to local environmental regulations. If the isolation valve O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw.
Page 117 Figure 4–21: Removing the PEEK terminal block and ceramic heater mounting block PEEK terminal block Ceramic heater mounting block 11. Use the O-ring removal kit to carefully remove the cover seal from the ion block (see also Removing O-rings and seals).
Page 118: Cleaning The Ion Block Components
Warning: To avoid spreading contamination, dispose of all waste materials according to local environmental regulations. 13. If the cover seal or cone gas O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. 4.14.3 Cleaning the ion block components Required materials •...
Page 119: Assembling The Source Ion Block Assembly
Rinse the components by immersing them separately in glass vessels containing water and then placing the vessels in the ultrasonic bath for 20 minutes. Dry the components by immersing them in separate glass vessels containing methanol and then placing the vessels in the ultrasonic bath for 10 minutes. Notice: To avoid recontaminating the components, wear clean, chemical- resistant, powder-free gloves for the rest of this procedure.
Page 120: Fitting The Ion Block Assembly To The Source Assembly
Carefully fit the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, to the ion block. Use the combined 2.5-mm Allen wrench and cone extraction tool to tighten the captive PEEK terminal block securing screw. Ensure that the grooves for the cover seal, cone gas O-ring, and isolation valve O-ring are free from dirt and debris.
Page 121: Cleaning The Stepwave Ion Guide Assembly
Warning: To avoid puncture wounds, take great care working with the source enclosure open if one or both of these conditions apply: • An ESI probe is fitted (the probe’s tip is sharp). • A corona pin is fitted (the pin’s tip is sharp). Notice: To avoid recontaminating the components, wear clean, chemical-resistant, powder-free gloves for the rest of this procedure.
Page 122 To remove the ion block support from the source assembly: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Remove the source enclosure from the instrument (see Removing the source enclosure from the instrument).
Page 123: Removing The Stepwave Assembly From The Source Assembly
Remove the PEEK ion block support from the adaptor housing. Use the O-ring removal kit to carefully remove all the O-rings from the PEEK ion block support (see Removing o-rings and seals). Warning: To avoid spreading contamination, dispose of all waste materials according to local environmental regulations.
Page 124 Figure 4–24: Seal breaker and locator tool: Handle June 9, 2016, 715004990 Rev. A Page 124...
Page 125 Figure 4–25: Seal breaker and locator tool positioned on the adaptor housing: Adaptor housing Ion guide cap Seal breaker and locator tool Push firmly on the seal breaker and locator tool’s handle, to lever the StepWave assembly slightly out of the adaptor housing. Rationale: Moving the assembly in this manner releases it from a seal located inside the instrument.
Page 126 Figure 4–26: StepWave assembly removal and insertion tool Ion block support screw holes (2) Brown PEEK ion guide cap Slot Cutout Pins Notice: To avoid damage when removing the StepWave ion guide assembly from the adaptor housing, handle only the brown PEEK ion guide cap Inserting your thumbs through the slots in the StepWave removal and insertion tool, pull the StepWave ion guide assembly from the pumping block assembly and into the StepWave removal and insertion tool.
Page 127: Disassembling The Stepwave Ion Guide Assembly
Using both hands, fit the source enclosure to the two supporting studs on the source adaptor housing. Close the source enclosure. Rationale: Fitting and closing the source enclosure prevents debris entering the instrument while you are working on the StepWave ion guide assembly. 4.15.4 Disassembling the StepWave ion guide assembly Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive...
Page 128 Second ion guide assembly Securing screw First ion guide assembly Separate the first and second ion guide assemblies. Figure 4–28: StepWave ion guide assembly separated Second ion guide assembly First ion guide assembly Remove the brown PEEK gasket from the second ion guide assembly. Figure 4–29: StepWave second ion guide assembly June 9, 2016, 715004990 Rev.
Page 129 Second ion guide assembly Brown PEEK gasket Remove the O-ring from the differential pumping aperture on the second ion guide assembly (see Removing O-rings and seals). Figure 4–30: StepWave second ion guide assembly Differential pumping aperture O-ring Warning: To avoid spreading contamination, dispose of all waste materials according to local environmental regulations.
Page 130: Cleaning The Stepwave Ion Guide Assembly
• Two lengths of PEEK, PTFE, or stainless-steel tubing, appropriately sized for suspending the first ion guide and second ion guide assemblies in the glass vessels when cleaning. • HPLC-grade deionized water • Waters MS Cleaning Solution (186006846) • Waste container • HPLC-grade isopropyl alcohol •...
Page 131 First ion guide PCB assembly Hook Add Waters MS Cleaning Solution to the glass vessel until the first ion guide PCB assembly is immersed completely. Repeat step 1 through step 3 for the second ion guide PCB assembly, placing the hook through one of the support rod holes.
Page 132: Assembling The Stepwave Ion Guide Assembly
Carefully pour the cleaning solution from the vessel holding the first ion guide PCB assembly into the holding container, retaining the ion guide in the vessel. Tip: You can reuse the cleaning solution for one subsequent cleaning. Fill the vessel with deionized water, to rinse the first ion guide PCB assembly, and then discard the water.
Page 133 To assemble the StepWave ion guide assembly: Notice: To avoid damaging the StepWave ion guide assembly, handle it and its components carefully throughout the cleaning procedure. In particular, to avoid damaging the wiring on assemblies fitted with an externally wired printed circuit board (PCB), do not touch the wiring.
Page 134: Fitting The Stepwave Assembly To The Source Assembly
Figure 4–34: Second ion guide assembly Brown PEEK gasket Align the first ion guide assembly with the second ion guide assembly. Use the combined, 2.5-mm, Allen wrench and cone extraction tool to fit and tighten the two screws securing the first ion guide assembly to the second ion guide assembly. 4.15.7 Fitting the StepWave assembly to the source assembly Required materials •...
Page 135 Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open the enclosure. Using both hands, grasp the source enclosure, and lift it vertically off the two supporting studs on the source adaptor housing. Slide the first ion guide assembly end of the StepWave assembly into the StepWave removal and insertion tool.
Page 136 Inserting your thumbs through the slots in the StepWave removal and insertion tool, firmly push the StepWave ion guide assembly into the pumping block assembly. Tip: You will detect some resistance to motion when the StepWave assembly encounters the seal inside the instrument; continue pushing until you detect further resistance. Remove the StepWave removal and insertion tool.
Page 137: Fitting The Ion Block Support To The Source
4.15.8 Fitting the ion block support to the source Required materials • Chemical-resistant, powder-free gloves • 3-mm hex wrench • New seals and O-rings To fit the PEEK ion block support to the source: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure.
Page 138 Warning: To avoid electric shock or solvent ignition, when connecting ESI or UPC source probes directly to non-Waters equipment, ensure that the liquid outlet connection is grounded. If the probe assembly is connected to the fluidics, open the access door to the IntelliStart...
Page 139: Replacing The Esi Probe Tip And Gasket
PEEK fitting Remove the probe assembly. Warning: To avoid spreading contamination, dispose of all waste materials according to local environmental regulations. Dispose of the probe assembly in accordance with local environmental regulations. To install a new probe assembly, see Installing the probe assembly.
Page 140 To remove the ESI probe tip and gasket: Warning: To avoid personal contamination with biohazards, wear clean, chemical- resistant, powder-free gloves when performing this procedure. Warning: To avoid burn injuries, take great care while performing this procedure. Warning: To avoid puncture injuries, handle sample needles, syringes, fused silica lines, and borosilicate tips with extreme care.
Page 141: Fitting The Esi Probe Tip And Gasket
Figure 4–39: Metal gasket Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of all waste materials according to local environmental regulations. Dispose of the metal gasket in accordance with local environmental regulations. If the probe tip is damaged, dispose of it in accordance with local environmental regulations.
Page 142: Cleaning The Apci Probe Tip
Figure 4–40: Inserting the gasket Metal gasket ESI probe tip Stainless steel tube Fit the probe tip, and screw the tip onto the probe assembly. Use the 7-mm wrench in conjunction with the 10-mm wrench to tighten the probe tip. Important: To avoid gas leakage, fully tighten the probe tip.
Page 143: Replacing The Apci Probe Heater
4.19 Replacing the APCI probe heater Replace the APCI probe heater it fails to heat the probe. 4.19.1 Removing the APCI probe heater Required materials Chemical-resistant, powder-free gloves To remove the APCI probe heater: Warning: To avoid burn injuries, take great care while working with the probe and source;...
Page 144: Fitting The New Apci Probe Heater
Notice: To avoid damaging the probe heater's electrical connections, do not twist the heater when removing it from or refitting it to the probe adaptor. Warning: To avoid burn injuries, take great care while performing this procedure. Gripping the probe heater as shown, carefully pull it off the probe adaptor. Figure 4–42: Probe heater Warning:...
Page 145 Figure 4–43: Fitting the probe heater Capillary sleeve Probe heater connections Notice: To avoid damaging the probe heater's electrical connections, do not twist the heater when removing it from or refitting it to the probe adaptor. Fit the probe adaptor to the instrument (see Installing the probe adaptor).
Page 146: Cleaning Or Replacing The Corona Pin
4.20 Cleaning or replacing the corona pin Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers • HPLC-grade methanol • Lint-free tissue • Lapping film • Corona pin To clean or replace the corona pin: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure.
Page 147 Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers • Combined 2.5-mm hex wrench and cone extraction tool • New ion block source heater assembly To replace the ion block source heater: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure.
Page 148 Figure 4–45: Ion block cover plate securing screw Ion block cover plate Remove the ion block cover plate. Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw. Figure 4–46: PEEK terminal block securing screw Heater cartridge assembly wires Notice: To avoid damaging the heater cartridge assembly wires, do not bend or...
Page 149 Carefully remove the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, from the ion block. Tip: You can invert the ion block assembly to facilitate this process. Figure 4–47: Heater wire securing screws PEEK terminal block Ceramic heater mounting block Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the two screws securing the heater wires to the PEEK terminal block.
Page 150: Replacing The Source Assembly Seals
Heater cartridge assembly Ceramic heater mounting block Heat-shrink tubing 10. Dispose of the heater cartridge assembly. Notice: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when removing the assembly and ceramic heater mounting block from the ion block.
Page 151: Removing The Probe Adjuster Assembly Probe And Source Enclosure Seals
• Source enclosure seal • Nebulizer gas seal • Desolvation gas seal 4.22.1 Removing the probe adjuster assembly probe and source enclosure seals Required materials • Chemical-resistant, powder-free gloves • O-ring removal kit To remove the probe adjuster assembly probe and source enclosure seals: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure.
Page 152 Probe adjuster nebulizer gas seal Probe adjuster assembly probe seal Use the O-ring removal kit to carefully remove the following seals from the source enclosure: • Source enclosure seal • Nebulizer gas seal • Desolvation gas seal Figure 4–50: Source enclosure seals Desolvation gas seal Source enclosure seal Nebulizer gas seal...
Page 153: Fitting The New Source Enclosure And Probe Adjuster Assembly Probe Seals
4.22.2 Fitting the new source enclosure and probe adjuster assembly probe seals Required materials • Chemical-resistant, powder-free gloves • Wash bottle containing HPLC-grade (or better) 1:1 methanol/water • New seals • Lint-free cloth To fit the new source enclosure and probe adjuster assembly probe seals: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure.
Page 154: Replacing The Air Filter Inside The Front Door
• Needle-nose pliers • New filter To replace the air filter inside the front door: Open the access door to the fluidics pump (see the figure on Waters ACQUITY Xevo TQ-S UPLC/MS system). Unscrew the captive thumbscrew on the filter cover.
Page 155 Figure 4–53: Filter Filter cover Lift the filter, vertically, from its slot in the instrument. Tip: If necessary, use the needle-nose pliers to grasp the filter. Figure 4–54: Dispose of the filter. Fit the new filter into the instrument. Fit the filter cover to the instrument. Tighten the thumbscrew on the filter cover.
Page 156: Appi/Apci Source - Changing The Uv Lamp Bulb
4.24 APPI/APCI source - changing the UV lamp bulb Required materials: • Chemical-resistant, powder-free gloves • Combined 2.5-mm hex wrench and cone extraction tool • Phillips screwdriver • 20-cm (8-inch) length of 4-mm nylon tube Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure.
Page 157 Figure 4–55: Note: To avoid breaking the bulb, do not use a screwdriver to push the bulb forward in the lamp drive assembly. Insert the length of 4-mm nylon tube through the back of the lamp drive assembly, and push the bulb forward. Figure 4–56: APPI lamp bulb Nylon tube...
Page 158: Appi/Apci Source-Cleaning The Lamp Window
4.25 APPI/APCI source—cleaning the lamp window The transmission of the high-energy photons responsible for APPI relies on the cleanliness of the magnesium fluoride lamp window. Clean the window to keep the surface clear of contamination, and avoid reduced sensitivity. Required materials •...
Page 159: Removing The Appi Lamp Drive Assembly Seals
• UV lamp bulb sealing O-ring • Mounting shaft O-rings • UV lamp mounting flange O-ring Tip: An automatic pressure test is performed each time the source enclosure is closed and when the instrument starts. 4.26.1 Removing the APPI lamp drive assembly seals Required materials •...
Page 160 Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the two lamp- drive cover screws (located above the bulb-extraction plug-aperture). Note: To avoid damaging the source enclosure, take care to lay it on a smooth surface. Laying the source enclosure face-first on a hard object or other protrusion can smash the glass window.
Page 161 Figure 4–58: Mounting-flange screw Lamp mounting flange 12. Slide the lamp assembly, shaft, and flange out of the APPI source enclosure. Tip: The cables remain attached to the shaft, which you fully withdraw and lay on the bench beside the source enclosure. Figure 4–59: Repeller electrode Electrode screw...
Page 162 14. Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the two insulator screws. 15. Remove the PEEK insulator from the end of the mounting shaft. 16. Slide the shaft mounting flange off the shaft, and note the correct orientation, for its reassembly.
Page 163: Fitting The New Appi Lamp Drive Assembly O-Rings
Figure 4–62: Lamp mounting flange O-ring Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of all waste materials according to local environmental regulations. 20. Dispose of the O-rings in accordance with local environmental regulations. 4.26.2 Fitting the new APPI lamp drive assembly O-rings Required materials •...
Page 164 Note: To avoid damaging the APPI lamp drive assembly O-rings, take care when fitting them. Small nicks, tears, dirt, and dust can compromise their performance, leading to rapid deterioration in the assembly’s operation. Ensure that all the grooves for the O-rings are free from dirt and hairs. Tip: If contamination is present, use 1:1 methanol/water, applied to the lint-free cloth, to carefully clean the grooves.
Page 165: Replacing The Unispray Probe Assembly
11. Use the combined 2.5-mm Allen wrench and cone extraction tool to fit and tighten the two insulator retaining screws. 12. Fit the repeller electrode to the PEEK insulator. 13. Use the small Phillips screwdriver to fit and tighten the repeller electrode retaining screw. 14.
Page 166: Fitting The Unispray Probe Assembly
Figure 4–64: UniSpray probe assembly PEEK fitting Probe fitting Probe capillary Unscrew the probe fitting and pull the probe assembly out of the shaft inlet atop the source enclosure. Warning: To avoid lacerations, puncture injuries, and possible contamination with biohazardous and toxic samples, do not touch the sharp end of the capillary. Dispose of the tubes and fittings in accordance with local environmental regulations.
Page 167 To replace the UniSpray probe assembly: Prepare the instrument for working on the source (see Preparing the instrument for working on the source). Warning: To avoid burn injuries, exercise care when handling the components of the source enclosure heated to high temperatures. Wait until the hot components have sufficiently cooled before you handle them.
Page 168: Maintaining The Unispray Impactor Pin
4.28 Maintaining the UniSpray impactor pin UniSpray directs the spray at an impactor pin held at a voltage, creating smaller charged droplets, amenable to easy desolvation. This section provides details about removing, cleaning, and installing the UniSpray impactor pin. Figure 4–66: UniSpray Impactor pin Impactor pin mounting block Impactor pin Pin handle...
Page 169: Cleaning Or Replacing The Unispray Impactor Pin
Unscrew the pin handle and pull it to withdraw the pin from the mounting block. Dispose of the used pin in accordance with local environmental regulations, or clean the pin, according to the procedure in Cleaning the UniSpray impactor pin. Holding the handle of the pin you are installing, insert the pin through the inlet on the mounting block and screw the handle into the mounting block.
Page 170: Replacing The Fluidic Lines Of The Ionkey Source
Notice: Do not rotate the impactor pin when wiping it in the lapping film or when wiping the pin clean, because this can damage the pin. Install the impactor pin in the UniSpray source (see Removing and installing the UniSpray impactor pin).
Page 171: Removing A Fluidic Line
Tip: Part numbers are embossed on the shrink-wrap below the ferrule assembly. You must disconnect the line from the source to see the part number. 4.29.1 Removing a fluidic line Required Materials • Chemical-resistant, powder-free gloves • Flat-blade screwdriver • T10 TORX driver Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure.
Page 172 Figure 4–68: iKey surround Unlock and remove any iKey installed in the iKey clamp. Remove the gas line using a flat-blade screwdriver. June 9, 2016, 715004990 Rev. A Page 172...
Page 173 Figure 4–69: Gas line Disconnect the fluidic line being replaced from its source. Remove two T10 Torx screws securing the end plate bracket. June 9, 2016, 715004990 Rev. A Page 173...
Page 174 Figure 4–70: T10 Torx screw End plate bracket T10 Torx screw Slide the end plate, along with the capillary tubing, out of the iKey clamp. Gently pull the O-ring from around the tube’s fitting and slide the O-ring to the left. Pull enough of the line being replaced to the right and allow the capillary line to slide through its channel to remove it.
Page 175: Installing A Fluidic Line
10. Remove the O-ring from the tubing. 11. Dispose of the removed tubing in accordance with standard laboratory procedures for contaminated vessels and sharps. 4.29.2 Installing a fluidic line Required Materials • Chemical-resistant, powder-free gloves • Fluid line assembly Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure.
Page 176: Cleaning The Ionkey Source And Connectors
Secure the fitting with the O-ring. Reinstall the end plate, end plate bracket, and gas line. Route the open end of the fluidic line through the fluid line aperture on the left of the source. Reinstall the fitting, ferrule, and lock ring onto the end of the new fluidic line, then connect it to the ACQUITY UPLC M-Class system, or to the onboard IntelliStart Fluidics on the mass spectrometer (see the ionKey and TRIZAIC source...
Page 177: To Remove Buildup From Electronic Connectors
During normal operation, the ionKey source does not require cleaning. After repeated use, however, material can accumulate around the fluid-line connectors or electronic connectors in the iKey docking port. Figure 4–73: Electronic connectors Fluid line connectors To remove buildup from fluid-line connectors: Gently swab the fluid-line connectors and outer edges of the docking port with a lint-free tissue saturated with isopropanol or methanol.
Page 178: To Clean The Outside Surfaces Of The Ionkey Source
4.30.2 To clean the outside surfaces of the ionKey source To clean the outside surfaces of the ionKey source: Use a lint-free tissue, dampened with water. Clean the other system components according to the supplied documentation. June 9, 2016, 715004990 Rev. A Page 178...
Page 179: A Safety Advisories
Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices.
Page 180: Specific Warnings
(Risk of explosion.) A.1.1 Specific warnings A.1.1.1 Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized: •...
Page 181 LC solvent should the nitrogen supply fail. A.1.1.4 Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans.
Page 182: Notices
Warning: To avoid personal contamination with biohazards, toxic materials, or corrosive materials, you must understand the hazards associated with their handling. Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Management of Chemical Hazards.
Page 183: Warnings That Apply To All Waters Instruments And Devices
A.5 Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Warning: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
Page 184 Warning: Use caution when working with any polymer tubing under pressure: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. • Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids.
Page 185 Avvertenza: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante. • Non utilizzare tubi eccessivamente logorati o piegati. •...
Page 186 い。 • 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合があり、その場合、チューブは極めて低い圧力で破裂します。 This warning applies to Waters instruments fitted with nonmetallic tubing. This warning applies to instruments operated with flammable solvents. Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.
Page 187: Warnings That Address The Replacing Of Fuses
警告：使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用，那么该设备所提供的保护将被削弱。 경고: 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다. 警告: ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供している保証が無効になる可能性があることに注意して下さい。 A.6 Warnings that address the replacing of fuses The following warnings pertain to instruments and devices equipped with user-replaceable fuses. Information describing fuse types and ratings sometimes, but not always, appears on the instrument or device.
Page 188: Electrical Symbols
警告: 火災予防のために、ヒューズ交換では機器ヒューズカバー脇のパネルに記載されているタイプおよび定格のヒューズをご使用ください。 Finding fuse types and ratings when that information does not appear on the instrument or device: Warning: To protect against fire, replace fuses with those of the type and rating indicated in the “Replacing fuses” section of the Maintenance Procedures chapter. Avertissement : pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués dans la rubrique "Remplacement des...
Page 189: Handling Symbols
Symbol Description Electrical power off Standby Direct current Alternating current Alternating current (3 phase) Safety ground Frame, or chassis, terminal Fuse Functional ground Input Output A.8 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped.
Page 190 Symbol Description Use no hooks! Upper limit of temperature Lower limit of temperature Temperature limitation June 9, 2016, 715004990 Rev. A Page 190...
Page 191: B External Connections
To avoid damaging the mass spectrometer, observe the following precautions: • Contact Waters Technical Service before moving the instrument. • If you must transport the instrument, or remove it from service, contact Waters Technical Service for recommended cleaning, flushing, and packaging procedures.
Page 192: Connecting The Ebara Oil-Free Roughing Pump
Shielded Ethernet Video camera connection (for use with the optional NanoFlow ESI or IonKey source) Event inputs and outputs Power Roughing pump connectors Roughing pump grounding connnection Nitrogen exhaust Nitrogen inlet Pilot valve port Turbo vacuum Source vacuum Collision cell gas inlet B.2 Connecting the EBARA oil-free roughing pump To ensure proper ventilation, install the pump in a location that allows these clearance distances for each side:...
Page 193 Figure B–2: EBARA oil-free roughing pump Exhaust tubing Exhaust silencer Control signal connector Power connector Vacuum hose Required materials • Chemical-resistant, powder-free gloves • 7-mm nut driver • Sharp knife The following items are included in the installation kit: June 9, 2016, 715004990 Rev. A Page 193...
Page 194 • NW25 center rings • NW25 clamps • NW40 center rings • NW40 clamps • NW40 elbow • NW40 tee piece • NW40 to NW16 reducer • NW40 to NW25 reducer • 12.7-mm clear PVC exhaust tubing • PVC hose clamps •...
Page 195 Figure B–3: Roughing pump tubing connections to rear of the Xevo TQ-XS mass spectrometer Roughing pump electrical cable attached to vacuum hose Vacuum hose to roughing pump NW40 clamps NW25 clamps Flexible vacuum tubing NW40/NW25 reducer NW40 tee piece NW40 elbow June 9, 2016, 715004990 Rev.
Page 196 Figure B–4: Roughing pump tubing connections to the EBARA pump Exhaust tubing NW40/NW16 reducer Exhaust silencer NW40 clamps Vacuum hose from rear of the Xevo TQ-XS Make the electrical connections to the roughing pump (see Making connections to the Ebara oil-free roughing pump).
Page 197: Making The Electrical Connections To The Ebara Oil-Free Roughing Pump
Tape attaching electrical cables to the vacuum hose Grounding connector Backing pump control connector Electronics ON/OFF switch (for use by Waters engineers only) Pump override switch (for use by Waters engineers only) To make the electrical connections for the oil-free roughing pump: Connect the roughing pump power cord to the main power source.
Page 198: Connecting To The Nitrogen Gas Supply
Tip: Use only the upper backing pump control connector. Leave the lower connector marked Delayed backing pump control, disconnected. Connect the grounding cable to the grounding connection. B.4 Connecting to the nitrogen gas supply Required materials • Chemical-resistant, powder-free gloves •...
Page 199: Connecting To The Collision Cell Gas Supply
Source vacuum Collision cell gas inlet Attach a nitrogen regulator to the nitrogen supply. Requirement: The nitrogen must be dry and oil-free, with a purity of at least 95%. Connect the free end of the 6-mm PTFE tubing to the nitrogen regulator. Set the nitrogen regulator to 690 kPa (6.9bar, 100 psi).
Page 200 • Nitrogen exhaust trap bottle • 4-mm PTFE tubing and 12-mm PTFE tubing (included in the Xevo TQ-XS installation kit) To connect the nitrogen exhaust line: Warning: To prevent the nitrogen exhaust from carrying biologically hazardous, toxic, or corrosive LC solvents, you must use a nitrogen exhaust trap bottle and a laboratory exhaust system.
Page 201: Connecting Liquid Waste Lines
To laboratory exhaust port From instrument pilot valve port Bottle support Nitrogen exhaust trap bottle From instrument exhaust connection One-way valve Notice: To avoid gas leaks, use the sharp knife to cut the PVC exhaust tubing squarely (that is, perpendicular to its horizontal axis). Cut a length of 4-mm tubing long enough to connect the instrument to the nitrogen exhaust trap bottle.
Page 202 Required materials • Chemical-resistant, powder-free gloves • Waste container To connect the liquid waste line: Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves when performing this procedure. Place a suitable waste container below the mass spectrometer. Notice: To avoid distorting the drip tray or causing the drain cup to leak, restrain the drain cup when attaching or removing the waste line.
Page 203 Warning: To prevent leakage of biologically hazardous, toxic, or corrosive materials, • do not crimp or bend drain lines. A crimp or bend can impede flow to the waste container. • empty the waste container before the lower end of the drain tubes are covered by waste solvent.
Page 204: Connecting The Workstation (Systems With No Acquity Lc)
B.8 Connecting the workstation (systems with no ACQUITY Before connecting the workstation to the instrument, set up the workstation according to its accompanying instructions. Locate the workstation within three meters (ten feet) of the mass spectrometer. Requirement: Use shielded network cables with the mass spectrometer to ensure compliance with FCC limits.
Page 205: Input/Output Signal Connectors
B.10 Input/output signal connectors Warning: To avoid electric shock, all electrical connections to the rear panel must be separated from hazardous voltages by double or reinforced insulation. Circuits of this type are classified as safety extra low voltage (SELV). Examples of circuits that are typically SELV include contact closure inputs and outputs for auto-samplers, and UV, RI, and fluorescence detector signal outputs for LC/MS systems.
Page 206 Figure B–11: Input/output signal connector configuration: Table B–1: External connections 1: Function Rating Event In 1+, digital signal, optimum +3.3V max +5V +5 V Event In 1-, digital ground, 0V Not used Event In 2+, digital signal, optimum +3.3V max +5V +5 V Event In 2-, digital ground, 0V Not used...
Page 207: Connecting To The Electricity Source
HAR-type (or better) cord in Europe. The main power cord must be replaced only with one of adequate rating. For information regarding what cord to use in other countries, contact your local Waters distributor. Connect the female end of the power cord to the receptacle on the rear panel of the mass spectrometer.
Page 208: C Materials Of Construction And Compatible Solvents
For information on preventing contamination, refer to Controlling Contamination in UltraPerformance LC/MS and HPLC/MS Systems (part number 715001307). You can find this document on www.waters.com; click Services and Support > Support. C.2 Items exposed to solvent The items that appear in the following table can be exposed to solvent. You must evaluate the safety issues if the solvents used in your application differ from the solvents typically used with these items.
Page 209: Solvents Used To Prepare Mobile Phases
Table C–1: Items exposed to solvent: (continued) Item Material Solvent waste/leak management Tygon tubing 2375, polyurethane Source enclosure Aluminium Source enclosure view port Silica float glass Trap bottle Polypropylene Trap bottle push-in fittings Nitrile butadiene rubber, stainless steel, polybutylene terephthalate, and polyoxymethylene APPI lamp drive assembly APPI lamp drive mounting shaft Stainless steel...
Page 210 solvents are used as additives to the mobile phases at reduced concentration, or as sample diluents. June 9, 2016, 715004990 Rev. A Page 210...
Page 211: D Intellistart Fluidics System Plumbing
For information on preventing contamination, refer to Controlling Contamination in UltraPerformance LC/MS and HPLC/MS Systems (part number 715001307). You can find this document on www.waters.com; click Services and Support > Support. D.2 Plumbing schematic For further information, refer to the diagram on the inside of the fluidics valve access door (see Waters ACQUITY Xevo TQ-XS UPLC/MS system).
Page 212: Ionkey And Trizaic Source Plumbing
ACQUITY system Source Waste Wash Vial C Vial B Vial A Analyte pump Selector valve Diverter valve D.3 ionKey and TRIZAIC source plumbing Figure D–2: ionKey and TRIZAIC source plumbing June 9, 2016, 715004990 Rev. A Page 212...
Page 213: Tubing Specifications
TRIZAIC tile or iKey Syringe Emitter HTM trap valve (TRIZAIC only) Waste nanoACQUITY UPLC SM, AQUITY UPLC M-class µSM, or trap device ionKey or TRIZAIC source D.4 Tubing specifications The following table gives the internal diameter (ID), external diameter (ED), color, length, and quantity for the IntelliStart Fluidics tubing.

Waters Xevo TQ-XS Mass Overview And Maintenance Manual

General Information

1 Waters Xevo TQ-XS Overview

2 Preparing the Mass Spectrometer for Operation

3 Changing the Mode of Operation

4 Maintenance Procedures

A Safety Advisories

B External Connections

C Materials of Construction and Compatible Solvents

D Intellistart Fluidics System Plumbing

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