Zeiss Axioskop 2 plus Operating Manual page 93

Axioskop 2 plus
Axioskop 2 mot plus
• Swing in 10x objective (yellow ring, see p. 1-12)
in the nosepiece
specimen via drive (3-17/2).
• Close luminous-field diaphragm (3-17/4) until it
becomes visible (even if not in focus) in the field
of view (3-17/A).
• Use drive for height adjustment (3-18/2) to
lower condenser until the edge of the
luminous-field diaphragm appears in focus
(3-17/B).
• Use both centering screws (3-18/3) of the
universal condenser to center the luminous-field
diaphragm (3-17/C)
diaphragm until its edge just disappears from
the field of view (3-17/D).
• For aperture diaphragm setting (contrast),
remove one eyepiece from the tube and look
into the tube with your naked eye. Use sliding
knob (3-17/5) to set the aperture diaphragm to
approx. 2/3 ... 4/5 of the diameter of the
objective exit pupils
applications, this aperture diaphragm setting
provides optimum contrast at almost ideal
resolution, and
compromise for the human eye.
• Insert eyepiece in the tube again.
☞
Since field size and objective aperture change after every objective change, the setting of the
luminous-field diaphragm and the aperture diaphragm must be repeated to obtain optimum
results.
For < 10x objectives, the front lens of the condenser must be folded out and the aperture
diaphragm fully opened. In the case of such large fields, the luminous-field diaphragm can also
be used for better contrasting by reducing its opening until it becomes visible in the field of
view.
B 40-075 e 02/01
Illumination and contrasting techniques
(3-17/6) and focus on the
and then open
(3-17/E). In
is therefore the
OPERATION
the
most
best
Fig. 3-17
4 0
9 0
Microscope settings on the
Axioskop 2 plus in transmitted-light
brightfield
Carl Zeiss
3-25